Influence of iodoform on antimicrobial potential of calcium hydroxide

Detalhes bibliográficos
Autor(a) principal: Estrela, Carlos
Data de Publicação: 2006
Outros Autores: Estrela, Cyntia Rodrigues de Araújo, Hollanda, Augusto César Braz, Decurcio, Daniel de Almeida, Pécora, Jesus Djalma
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/3339
Resumo: The purpose of this research was to verify the influence of Iodoform on antimicrobial potential of calcium hydroxide. S. aureus, E. faecalis, P. aeruginosa, B. subtilis, C. albicans were the biological indicators. The substances tested were: calcium hydroxide + saline; calcium hydroxide + Iodoform + saline; Iodoform + saline. For the agar diffusion test, 18 Petri plates with 20 ml of BHI agar were inoculated with the microbial suspensions. Fifty-four cavities were made and filled with the substances tested. The diameters of microbial inhibition were then measured. In direct exposure test, 162 #50 sterile absorbent paper points were immersed in the experimental suspensions for 5 min, and covered with the pastes. At intervals of 24, 48 and 72 hours, the paper points were immersed in 10 ml of Letheen Broth, followed by incubation at 37°°C for 48h. Microbial growth was evaluated by turbidity of the culture medium. A 0.1 ml inoculum obtained from the Letheen Broth was transferred to 7 ml of BHI, and incubated at 37°°C for 48h. Bacterial growth was again evaluated by turbidity of the culture medium. The calcium hydroxide associated with the saline or the iodoform plus saline showed antimicrobial effectiveness in both experimental methods. The iodoform paste presented antimicrobial ineffectiveness for the agar diffusion test on all biological microorganisms and for the direct exposure test on B. subtilis and on the mixture.
id USP-17_238b405bcb819368d2e59177848a768c
oai_identifier_str oai:revistas.usp.br:article/3339
network_acronym_str USP-17
network_name_str Journal of applied oral science (Online)
repository_id_str
spelling Influence of iodoform on antimicrobial potential of calcium hydroxide Influência do iodofórmio no potencial antimicrobiano do hidróxido de cálcio Hidróxido de cálcioIodofórmioMedicação intracanalCalcium hydroxideIodoformIntracanal medicaments The purpose of this research was to verify the influence of Iodoform on antimicrobial potential of calcium hydroxide. S. aureus, E. faecalis, P. aeruginosa, B. subtilis, C. albicans were the biological indicators. The substances tested were: calcium hydroxide + saline; calcium hydroxide + Iodoform + saline; Iodoform + saline. For the agar diffusion test, 18 Petri plates with 20 ml of BHI agar were inoculated with the microbial suspensions. Fifty-four cavities were made and filled with the substances tested. The diameters of microbial inhibition were then measured. In direct exposure test, 162 #50 sterile absorbent paper points were immersed in the experimental suspensions for 5 min, and covered with the pastes. At intervals of 24, 48 and 72 hours, the paper points were immersed in 10 ml of Letheen Broth, followed by incubation at 37°°C for 48h. Microbial growth was evaluated by turbidity of the culture medium. A 0.1 ml inoculum obtained from the Letheen Broth was transferred to 7 ml of BHI, and incubated at 37°°C for 48h. Bacterial growth was again evaluated by turbidity of the culture medium. The calcium hydroxide associated with the saline or the iodoform plus saline showed antimicrobial effectiveness in both experimental methods. The iodoform paste presented antimicrobial ineffectiveness for the agar diffusion test on all biological microorganisms and for the direct exposure test on B. subtilis and on the mixture. Objetivo deste estudo foi verificar a influência do Iodofórmio no potencial antimicrobiano do hidróxido de cálcio. S. aureus, E. faecalis, P. aeruginosa, B. subtilis, C. albicans foram os indicadores biológicos. As substâncias testadas foram: hidróxido de cálcio + solução salina; hidróxido de cálcio + iodofórmio + solução salina; iodofórmio + solução salina. Para o teste de difusão em ágar, 18 placas de Petri contendo 20 ml de ágar BHI foram inoculadas com as suspensões microbianas. Cinqüenta e quatro cavidades foram feitas e preenchidas com as substâncias testadas. Os diâmetros da inibição microbiana foram então mensurados. No teste de exposição direta, 162 pontas de papel absorvente número 50 esterilizadas foram imersas nas suspensões experimentais por 5 minutos, e cobertas pelas pastas testadas. Em intervalos de 24, 48 e 72 horas, as pontas de papel foram imersas em 10 ml de Letheen Broth, seguido de incubação a 37°°C por 48 horas. O crescimento microbiano foi avaliado pela turvação do meio de cultura. Um inóculo de 0.1 ml obtido do Letheen Broth foi transferido para 7 ml de BHI, e incubado a 37°°C por 48h. o crescimento bacteriano foi novamente avaliado pela turvação do meio de cultura. As pastas contendo hidróxido de cálcio e solução salina, hidróxido de cálcio-iodofórmio e solução salina mostraram significativa atividade antimicrobiana nos métodos experimentais estudados. A pasta contendo iodofórmio e solução salina foi inefetiva pelo teste de difusão em agar e, também, por exposição direta, para o B. subtilis e a mistura. Universidade de São Paulo. Faculdade de Odontologia de Bauru2006-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/333910.1590/S1678-77572006000100007Journal of Applied Oral Science; Vol. 14 No. 1 (2006); 33-37 Journal of Applied Oral Science; Vol. 14 Núm. 1 (2006); 33-37 Journal of Applied Oral Science; v. 14 n. 1 (2006); 33-37 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3339/4029Copyright (c) 2006 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessEstrela, CarlosEstrela, Cyntia Rodrigues de AraújoHollanda, Augusto César BrazDecurcio, Daniel de AlmeidaPécora, Jesus Djalma2012-04-27T11:52:53Zoai:revistas.usp.br:article/3339Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T11:52:53Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Influence of iodoform on antimicrobial potential of calcium hydroxide
Influência do iodofórmio no potencial antimicrobiano do hidróxido de cálcio
title Influence of iodoform on antimicrobial potential of calcium hydroxide
spellingShingle Influence of iodoform on antimicrobial potential of calcium hydroxide
Estrela, Carlos
Hidróxido de cálcio
Iodofórmio
Medicação intracanal
Calcium hydroxide
Iodoform
Intracanal medicaments
title_short Influence of iodoform on antimicrobial potential of calcium hydroxide
title_full Influence of iodoform on antimicrobial potential of calcium hydroxide
title_fullStr Influence of iodoform on antimicrobial potential of calcium hydroxide
title_full_unstemmed Influence of iodoform on antimicrobial potential of calcium hydroxide
title_sort Influence of iodoform on antimicrobial potential of calcium hydroxide
author Estrela, Carlos
author_facet Estrela, Carlos
Estrela, Cyntia Rodrigues de Araújo
Hollanda, Augusto César Braz
Decurcio, Daniel de Almeida
Pécora, Jesus Djalma
author_role author
author2 Estrela, Cyntia Rodrigues de Araújo
Hollanda, Augusto César Braz
Decurcio, Daniel de Almeida
Pécora, Jesus Djalma
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Estrela, Carlos
Estrela, Cyntia Rodrigues de Araújo
Hollanda, Augusto César Braz
Decurcio, Daniel de Almeida
Pécora, Jesus Djalma
dc.subject.por.fl_str_mv Hidróxido de cálcio
Iodofórmio
Medicação intracanal
Calcium hydroxide
Iodoform
Intracanal medicaments
topic Hidróxido de cálcio
Iodofórmio
Medicação intracanal
Calcium hydroxide
Iodoform
Intracanal medicaments
description The purpose of this research was to verify the influence of Iodoform on antimicrobial potential of calcium hydroxide. S. aureus, E. faecalis, P. aeruginosa, B. subtilis, C. albicans were the biological indicators. The substances tested were: calcium hydroxide + saline; calcium hydroxide + Iodoform + saline; Iodoform + saline. For the agar diffusion test, 18 Petri plates with 20 ml of BHI agar were inoculated with the microbial suspensions. Fifty-four cavities were made and filled with the substances tested. The diameters of microbial inhibition were then measured. In direct exposure test, 162 #50 sterile absorbent paper points were immersed in the experimental suspensions for 5 min, and covered with the pastes. At intervals of 24, 48 and 72 hours, the paper points were immersed in 10 ml of Letheen Broth, followed by incubation at 37°°C for 48h. Microbial growth was evaluated by turbidity of the culture medium. A 0.1 ml inoculum obtained from the Letheen Broth was transferred to 7 ml of BHI, and incubated at 37°°C for 48h. Bacterial growth was again evaluated by turbidity of the culture medium. The calcium hydroxide associated with the saline or the iodoform plus saline showed antimicrobial effectiveness in both experimental methods. The iodoform paste presented antimicrobial ineffectiveness for the agar diffusion test on all biological microorganisms and for the direct exposure test on B. subtilis and on the mixture.
publishDate 2006
dc.date.none.fl_str_mv 2006-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/3339
10.1590/S1678-77572006000100007
url https://www.revistas.usp.br/jaos/article/view/3339
identifier_str_mv 10.1590/S1678-77572006000100007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/3339/4029
dc.rights.driver.fl_str_mv Copyright (c) 2006 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2006 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 14 No. 1 (2006); 33-37
Journal of Applied Oral Science; Vol. 14 Núm. 1 (2006); 33-37
Journal of Applied Oral Science; v. 14 n. 1 (2006); 33-37
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
_version_ 1800221672678096896