Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin

Detalhes bibliográficos
Autor(a) principal: CARVALHO, Carolina
Data de Publicação: 2016
Outros Autores: FERNANDES, Fernando Pelegrim, FREITAS, Valeria da Penha, FRANÇA, Fabiana Mantovani Gomes, BASTING, Roberta Tarkany, TURSSI, Cecilia Pedroso, AMARAL, Flávia Lucisano Botelho
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/117205
Resumo: Objective Green tea extract has been advocated as a matrix metalloproteinase (MMP) inhibitor; however, its effect on bond durability to caries-affected dentin has never been reported. Thus, the aim of this in vitro study was to evaluate the effect of two MMP inhibitors (2% chlorhexidine and 2% green tea extract), applied after acid etching, on bond durability of an etch-and-rinse adhesive system to caries-affected dentin. Material and Methods Occlusal enamel was removed from third molars to expose the dentin surface, and the molars were submitted to a caries induction protocol for 15 days. After removal of infected dentin, specimens were conditioned with 37% phosphoric acid (15 seconds) and randomly divided into three groups, according to the type of dentin pretreatment (n=10): NT: no treatment; GT: 2% green tea extract; CLX: 2% chlorhexidine. The etch-and-rinse adhesive system (Adper™ Single Bond 2, 3M ESPE, St. Paul, MN, USA) was applied according to the manufacturer's instructions, and composite resin restorations were built on the dentin. After 24 hours, at 37°C, the resin-tooth blocks were sectioned perpendicularly to the adhesive interface in the form of sticks (0.8 mm2 of adhesive area) and randomly subdivided into two groups according to when they were to be submitted to microtensile bond strength (μTBS) testing: immediately or 6 months after storage in distilled water. Data were reported in MPa and submitted to two-way ANOVA for completely randomized blocks, followed by Tukey’s test (α=0.05). Results After 24 hours, there was no significant difference in the μTBS of the groups. After 6 months, the GT group had significantly higher μTBS values. Conclusion It was concluded that the application of 2% green tea extract was able to increase bond durability of the etch-and-rinse system to dentin. Neither the application of chlorhexidine nor non-treatment (NT - control) had any effect on bond strength after water storage.
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spelling Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin Objective Green tea extract has been advocated as a matrix metalloproteinase (MMP) inhibitor; however, its effect on bond durability to caries-affected dentin has never been reported. Thus, the aim of this in vitro study was to evaluate the effect of two MMP inhibitors (2% chlorhexidine and 2% green tea extract), applied after acid etching, on bond durability of an etch-and-rinse adhesive system to caries-affected dentin. Material and Methods Occlusal enamel was removed from third molars to expose the dentin surface, and the molars were submitted to a caries induction protocol for 15 days. After removal of infected dentin, specimens were conditioned with 37% phosphoric acid (15 seconds) and randomly divided into three groups, according to the type of dentin pretreatment (n=10): NT: no treatment; GT: 2% green tea extract; CLX: 2% chlorhexidine. The etch-and-rinse adhesive system (Adper™ Single Bond 2, 3M ESPE, St. Paul, MN, USA) was applied according to the manufacturer's instructions, and composite resin restorations were built on the dentin. After 24 hours, at 37°C, the resin-tooth blocks were sectioned perpendicularly to the adhesive interface in the form of sticks (0.8 mm2 of adhesive area) and randomly subdivided into two groups according to when they were to be submitted to microtensile bond strength (μTBS) testing: immediately or 6 months after storage in distilled water. Data were reported in MPa and submitted to two-way ANOVA for completely randomized blocks, followed by Tukey’s test (α=0.05). Results After 24 hours, there was no significant difference in the μTBS of the groups. After 6 months, the GT group had significantly higher μTBS values. Conclusion It was concluded that the application of 2% green tea extract was able to increase bond durability of the etch-and-rinse system to dentin. Neither the application of chlorhexidine nor non-treatment (NT - control) had any effect on bond strength after water storage. Universidade de São Paulo. Faculdade de Odontologia de Bauru2016-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/11720510.1590/1678-775720150518Journal of Applied Oral Science; Vol. 24 No. 3 (2016); 211-217Journal of Applied Oral Science; Vol. 24 Núm. 3 (2016); 211-217Journal of Applied Oral Science; v. 24 n. 3 (2016); 211-2171678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/117205/114806Copyright (c) 2016 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessCARVALHO, CarolinaFERNANDES, Fernando PelegrimFREITAS, Valeria da PenhaFRANÇA, Fabiana Mantovani GomesBASTING, Roberta TarkanyTURSSI, Cecilia PedrosoAMARAL, Flávia Lucisano Botelho2016-07-04T19:54:28Zoai:revistas.usp.br:article/117205Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2016-07-04T19:54:28Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin
title Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin
spellingShingle Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin
CARVALHO, Carolina
title_short Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin
title_full Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin
title_fullStr Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin
title_full_unstemmed Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin
title_sort Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin
author CARVALHO, Carolina
author_facet CARVALHO, Carolina
FERNANDES, Fernando Pelegrim
FREITAS, Valeria da Penha
FRANÇA, Fabiana Mantovani Gomes
BASTING, Roberta Tarkany
TURSSI, Cecilia Pedroso
AMARAL, Flávia Lucisano Botelho
author_role author
author2 FERNANDES, Fernando Pelegrim
FREITAS, Valeria da Penha
FRANÇA, Fabiana Mantovani Gomes
BASTING, Roberta Tarkany
TURSSI, Cecilia Pedroso
AMARAL, Flávia Lucisano Botelho
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv CARVALHO, Carolina
FERNANDES, Fernando Pelegrim
FREITAS, Valeria da Penha
FRANÇA, Fabiana Mantovani Gomes
BASTING, Roberta Tarkany
TURSSI, Cecilia Pedroso
AMARAL, Flávia Lucisano Botelho
description Objective Green tea extract has been advocated as a matrix metalloproteinase (MMP) inhibitor; however, its effect on bond durability to caries-affected dentin has never been reported. Thus, the aim of this in vitro study was to evaluate the effect of two MMP inhibitors (2% chlorhexidine and 2% green tea extract), applied after acid etching, on bond durability of an etch-and-rinse adhesive system to caries-affected dentin. Material and Methods Occlusal enamel was removed from third molars to expose the dentin surface, and the molars were submitted to a caries induction protocol for 15 days. After removal of infected dentin, specimens were conditioned with 37% phosphoric acid (15 seconds) and randomly divided into three groups, according to the type of dentin pretreatment (n=10): NT: no treatment; GT: 2% green tea extract; CLX: 2% chlorhexidine. The etch-and-rinse adhesive system (Adper™ Single Bond 2, 3M ESPE, St. Paul, MN, USA) was applied according to the manufacturer's instructions, and composite resin restorations were built on the dentin. After 24 hours, at 37°C, the resin-tooth blocks were sectioned perpendicularly to the adhesive interface in the form of sticks (0.8 mm2 of adhesive area) and randomly subdivided into two groups according to when they were to be submitted to microtensile bond strength (μTBS) testing: immediately or 6 months after storage in distilled water. Data were reported in MPa and submitted to two-way ANOVA for completely randomized blocks, followed by Tukey’s test (α=0.05). Results After 24 hours, there was no significant difference in the μTBS of the groups. After 6 months, the GT group had significantly higher μTBS values. Conclusion It was concluded that the application of 2% green tea extract was able to increase bond durability of the etch-and-rinse system to dentin. Neither the application of chlorhexidine nor non-treatment (NT - control) had any effect on bond strength after water storage.
publishDate 2016
dc.date.none.fl_str_mv 2016-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/117205
10.1590/1678-775720150518
url https://www.revistas.usp.br/jaos/article/view/117205
identifier_str_mv 10.1590/1678-775720150518
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/117205/114806
dc.rights.driver.fl_str_mv Copyright (c) 2016 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2016 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 24 No. 3 (2016); 211-217
Journal of Applied Oral Science; Vol. 24 Núm. 3 (2016); 211-217
Journal of Applied Oral Science; v. 24 n. 3 (2016); 211-217
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
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