Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/117205 |
Resumo: | Objective Green tea extract has been advocated as a matrix metalloproteinase (MMP) inhibitor; however, its effect on bond durability to caries-affected dentin has never been reported. Thus, the aim of this in vitro study was to evaluate the effect of two MMP inhibitors (2% chlorhexidine and 2% green tea extract), applied after acid etching, on bond durability of an etch-and-rinse adhesive system to caries-affected dentin. Material and Methods Occlusal enamel was removed from third molars to expose the dentin surface, and the molars were submitted to a caries induction protocol for 15 days. After removal of infected dentin, specimens were conditioned with 37% phosphoric acid (15 seconds) and randomly divided into three groups, according to the type of dentin pretreatment (n=10): NT: no treatment; GT: 2% green tea extract; CLX: 2% chlorhexidine. The etch-and-rinse adhesive system (Adper™ Single Bond 2, 3M ESPE, St. Paul, MN, USA) was applied according to the manufacturer's instructions, and composite resin restorations were built on the dentin. After 24 hours, at 37°C, the resin-tooth blocks were sectioned perpendicularly to the adhesive interface in the form of sticks (0.8 mm2 of adhesive area) and randomly subdivided into two groups according to when they were to be submitted to microtensile bond strength (μTBS) testing: immediately or 6 months after storage in distilled water. Data were reported in MPa and submitted to two-way ANOVA for completely randomized blocks, followed by Tukey’s test (α=0.05). Results After 24 hours, there was no significant difference in the μTBS of the groups. After 6 months, the GT group had significantly higher μTBS values. Conclusion It was concluded that the application of 2% green tea extract was able to increase bond durability of the etch-and-rinse system to dentin. Neither the application of chlorhexidine nor non-treatment (NT - control) had any effect on bond strength after water storage. |
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Journal of applied oral science (Online) |
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Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin Objective Green tea extract has been advocated as a matrix metalloproteinase (MMP) inhibitor; however, its effect on bond durability to caries-affected dentin has never been reported. Thus, the aim of this in vitro study was to evaluate the effect of two MMP inhibitors (2% chlorhexidine and 2% green tea extract), applied after acid etching, on bond durability of an etch-and-rinse adhesive system to caries-affected dentin. Material and Methods Occlusal enamel was removed from third molars to expose the dentin surface, and the molars were submitted to a caries induction protocol for 15 days. After removal of infected dentin, specimens were conditioned with 37% phosphoric acid (15 seconds) and randomly divided into three groups, according to the type of dentin pretreatment (n=10): NT: no treatment; GT: 2% green tea extract; CLX: 2% chlorhexidine. The etch-and-rinse adhesive system (Adper™ Single Bond 2, 3M ESPE, St. Paul, MN, USA) was applied according to the manufacturer's instructions, and composite resin restorations were built on the dentin. After 24 hours, at 37°C, the resin-tooth blocks were sectioned perpendicularly to the adhesive interface in the form of sticks (0.8 mm2 of adhesive area) and randomly subdivided into two groups according to when they were to be submitted to microtensile bond strength (μTBS) testing: immediately or 6 months after storage in distilled water. Data were reported in MPa and submitted to two-way ANOVA for completely randomized blocks, followed by Tukey’s test (α=0.05). Results After 24 hours, there was no significant difference in the μTBS of the groups. After 6 months, the GT group had significantly higher μTBS values. Conclusion It was concluded that the application of 2% green tea extract was able to increase bond durability of the etch-and-rinse system to dentin. Neither the application of chlorhexidine nor non-treatment (NT - control) had any effect on bond strength after water storage. Universidade de São Paulo. Faculdade de Odontologia de Bauru2016-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/11720510.1590/1678-775720150518Journal of Applied Oral Science; Vol. 24 No. 3 (2016); 211-217Journal of Applied Oral Science; Vol. 24 Núm. 3 (2016); 211-217Journal of Applied Oral Science; v. 24 n. 3 (2016); 211-2171678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/117205/114806Copyright (c) 2016 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessCARVALHO, CarolinaFERNANDES, Fernando PelegrimFREITAS, Valeria da PenhaFRANÇA, Fabiana Mantovani GomesBASTING, Roberta TarkanyTURSSI, Cecilia PedrosoAMARAL, Flávia Lucisano Botelho2016-07-04T19:54:28Zoai:revistas.usp.br:article/117205Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2016-07-04T19:54:28Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin |
title |
Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin |
spellingShingle |
Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin CARVALHO, Carolina |
title_short |
Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin |
title_full |
Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin |
title_fullStr |
Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin |
title_full_unstemmed |
Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin |
title_sort |
Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin |
author |
CARVALHO, Carolina |
author_facet |
CARVALHO, Carolina FERNANDES, Fernando Pelegrim FREITAS, Valeria da Penha FRANÇA, Fabiana Mantovani Gomes BASTING, Roberta Tarkany TURSSI, Cecilia Pedroso AMARAL, Flávia Lucisano Botelho |
author_role |
author |
author2 |
FERNANDES, Fernando Pelegrim FREITAS, Valeria da Penha FRANÇA, Fabiana Mantovani Gomes BASTING, Roberta Tarkany TURSSI, Cecilia Pedroso AMARAL, Flávia Lucisano Botelho |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
CARVALHO, Carolina FERNANDES, Fernando Pelegrim FREITAS, Valeria da Penha FRANÇA, Fabiana Mantovani Gomes BASTING, Roberta Tarkany TURSSI, Cecilia Pedroso AMARAL, Flávia Lucisano Botelho |
description |
Objective Green tea extract has been advocated as a matrix metalloproteinase (MMP) inhibitor; however, its effect on bond durability to caries-affected dentin has never been reported. Thus, the aim of this in vitro study was to evaluate the effect of two MMP inhibitors (2% chlorhexidine and 2% green tea extract), applied after acid etching, on bond durability of an etch-and-rinse adhesive system to caries-affected dentin. Material and Methods Occlusal enamel was removed from third molars to expose the dentin surface, and the molars were submitted to a caries induction protocol for 15 days. After removal of infected dentin, specimens were conditioned with 37% phosphoric acid (15 seconds) and randomly divided into three groups, according to the type of dentin pretreatment (n=10): NT: no treatment; GT: 2% green tea extract; CLX: 2% chlorhexidine. The etch-and-rinse adhesive system (Adper™ Single Bond 2, 3M ESPE, St. Paul, MN, USA) was applied according to the manufacturer's instructions, and composite resin restorations were built on the dentin. After 24 hours, at 37°C, the resin-tooth blocks were sectioned perpendicularly to the adhesive interface in the form of sticks (0.8 mm2 of adhesive area) and randomly subdivided into two groups according to when they were to be submitted to microtensile bond strength (μTBS) testing: immediately or 6 months after storage in distilled water. Data were reported in MPa and submitted to two-way ANOVA for completely randomized blocks, followed by Tukey’s test (α=0.05). Results After 24 hours, there was no significant difference in the μTBS of the groups. After 6 months, the GT group had significantly higher μTBS values. Conclusion It was concluded that the application of 2% green tea extract was able to increase bond durability of the etch-and-rinse system to dentin. Neither the application of chlorhexidine nor non-treatment (NT - control) had any effect on bond strength after water storage. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/117205 10.1590/1678-775720150518 |
url |
https://www.revistas.usp.br/jaos/article/view/117205 |
identifier_str_mv |
10.1590/1678-775720150518 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/117205/114806 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2016 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2016 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 24 No. 3 (2016); 211-217 Journal of Applied Oral Science; Vol. 24 Núm. 3 (2016); 211-217 Journal of Applied Oral Science; v. 24 n. 3 (2016); 211-217 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
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1800221679629107200 |