Multi-species biofilm of Candida albicans and non-Candida albicans Candida species on acrylic substrate
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/3988 |
Resumo: | OBJECTIVE: In polymicrobial biofilms bacteria extensively interact with Candida species, but the interaction among the different species of the Candida is yet to be completely evaluated. In the present study, the difference in biofilm formation ability of clinical isolates of four species of Candida in both single-species and multi-species combinations on the surface of dental acrylic resin strips was evaluated. MATERIAL AND METHODS: The species of Candida, isolated from multiple species oral candidiasis of the neutropenic patients, were used for the experiment. Organisms were cultured on Sabouraud dextrose broth with 8% glucose (SDB). Biofilm production on the acrylic resins strips was determined by crystal violet assay. Student's t-test and ANOVA were used to compare in vitro biofilm formation for the individual species of Candida and its different multi-species combinations. RESULTS: In the present study, differences between the mean values of the biofilm-forming ability of individual species (C. glabrata>;C. krusei>;C. tropicalis>;C. albicans) and in its multi-species' combinations (the highest for C. albicans with C. glabrata and the lowest for all the four species combination) were reported. CONCLUSIONS: The findings of this study showed that biofilm-forming ability was found greater for non-Candida albicans Candida species (NCAC) than for C. albicans species with intra-species variation. Presence of C. albicans in multi-species biofilms increased, whereas; C. tropicalis decreased the biofilm production with all other NCAC species. |
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Journal of applied oral science (Online) |
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Multi-species biofilm of Candida albicans and non-Candida albicans Candida species on acrylic substrate Oral candidiasisBiofilmsCrystal violetAssay OBJECTIVE: In polymicrobial biofilms bacteria extensively interact with Candida species, but the interaction among the different species of the Candida is yet to be completely evaluated. In the present study, the difference in biofilm formation ability of clinical isolates of four species of Candida in both single-species and multi-species combinations on the surface of dental acrylic resin strips was evaluated. MATERIAL AND METHODS: The species of Candida, isolated from multiple species oral candidiasis of the neutropenic patients, were used for the experiment. Organisms were cultured on Sabouraud dextrose broth with 8% glucose (SDB). Biofilm production on the acrylic resins strips was determined by crystal violet assay. Student's t-test and ANOVA were used to compare in vitro biofilm formation for the individual species of Candida and its different multi-species combinations. RESULTS: In the present study, differences between the mean values of the biofilm-forming ability of individual species (C. glabrata>;C. krusei>;C. tropicalis>;C. albicans) and in its multi-species' combinations (the highest for C. albicans with C. glabrata and the lowest for all the four species combination) were reported. CONCLUSIONS: The findings of this study showed that biofilm-forming ability was found greater for non-Candida albicans Candida species (NCAC) than for C. albicans species with intra-species variation. Presence of C. albicans in multi-species biofilms increased, whereas; C. tropicalis decreased the biofilm production with all other NCAC species. Universidade de São Paulo. Faculdade de Odontologia de Bauru2012-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/398810.1590/S1678-77572012000100013Journal of Applied Oral Science; Vol. 20 No. 1 (2012); 70-75 Journal of Applied Oral Science; Vol. 20 Núm. 1 (2012); 70-75 Journal of Applied Oral Science; v. 20 n. 1 (2012); 70-75 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3988/4678Copyright (c) 2012 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessPathak, Apurva KSharma, SanjayShrivastva, Pallavi2012-04-27T12:17:42Zoai:revistas.usp.br:article/3988Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T12:17:42Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Multi-species biofilm of Candida albicans and non-Candida albicans Candida species on acrylic substrate |
title |
Multi-species biofilm of Candida albicans and non-Candida albicans Candida species on acrylic substrate |
spellingShingle |
Multi-species biofilm of Candida albicans and non-Candida albicans Candida species on acrylic substrate Pathak, Apurva K Oral candidiasis Biofilms Crystal violet Assay |
title_short |
Multi-species biofilm of Candida albicans and non-Candida albicans Candida species on acrylic substrate |
title_full |
Multi-species biofilm of Candida albicans and non-Candida albicans Candida species on acrylic substrate |
title_fullStr |
Multi-species biofilm of Candida albicans and non-Candida albicans Candida species on acrylic substrate |
title_full_unstemmed |
Multi-species biofilm of Candida albicans and non-Candida albicans Candida species on acrylic substrate |
title_sort |
Multi-species biofilm of Candida albicans and non-Candida albicans Candida species on acrylic substrate |
author |
Pathak, Apurva K |
author_facet |
Pathak, Apurva K Sharma, Sanjay Shrivastva, Pallavi |
author_role |
author |
author2 |
Sharma, Sanjay Shrivastva, Pallavi |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Pathak, Apurva K Sharma, Sanjay Shrivastva, Pallavi |
dc.subject.por.fl_str_mv |
Oral candidiasis Biofilms Crystal violet Assay |
topic |
Oral candidiasis Biofilms Crystal violet Assay |
description |
OBJECTIVE: In polymicrobial biofilms bacteria extensively interact with Candida species, but the interaction among the different species of the Candida is yet to be completely evaluated. In the present study, the difference in biofilm formation ability of clinical isolates of four species of Candida in both single-species and multi-species combinations on the surface of dental acrylic resin strips was evaluated. MATERIAL AND METHODS: The species of Candida, isolated from multiple species oral candidiasis of the neutropenic patients, were used for the experiment. Organisms were cultured on Sabouraud dextrose broth with 8% glucose (SDB). Biofilm production on the acrylic resins strips was determined by crystal violet assay. Student's t-test and ANOVA were used to compare in vitro biofilm formation for the individual species of Candida and its different multi-species combinations. RESULTS: In the present study, differences between the mean values of the biofilm-forming ability of individual species (C. glabrata>;C. krusei>;C. tropicalis>;C. albicans) and in its multi-species' combinations (the highest for C. albicans with C. glabrata and the lowest for all the four species combination) were reported. CONCLUSIONS: The findings of this study showed that biofilm-forming ability was found greater for non-Candida albicans Candida species (NCAC) than for C. albicans species with intra-species variation. Presence of C. albicans in multi-species biofilms increased, whereas; C. tropicalis decreased the biofilm production with all other NCAC species. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3988 10.1590/S1678-77572012000100013 |
url |
https://www.revistas.usp.br/jaos/article/view/3988 |
identifier_str_mv |
10.1590/S1678-77572012000100013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3988/4678 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2012 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2012 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 20 No. 1 (2012); 70-75 Journal of Applied Oral Science; Vol. 20 Núm. 1 (2012); 70-75 Journal of Applied Oral Science; v. 20 n. 1 (2012); 70-75 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221676931121152 |