Evaluation of cytotoxicity of two endodontic cements in a macrophage culture
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572005000300007 |
Resumo: | Compared to gutta-percha, the endodontic cements are used in small quantity to seal root canals, but are indispensable to achieve hermetically sealed margins, where its biocompatibility depends on the sum of responses of each cell present in the periapical region. The object of this study was to evaluate the cytotoxicity of two endodontic cements, one based on epoxy resin (Sealer 26) and the other containing zinc oxide eugenol (Endofill) by using cultured peritoneal macrophages from Swiss mice to measure the induced production of nitric oxide. After solidification and pulverization, aliquots of 100mul of suspension containing 18mg/mL of the respective cements were added to 96-well tissue culture plates containing the tissue culture of macrophages at a concentration of 5.0X10(6) cells/ml. In the positive control group the cell culture was treated with 10mg/mL of lipopolyssaccharide from Escherichia coli 026:B6 and the cell culture alone represented the negative control. After 48 hours of incubation, at 37ºC, in 5% CO2, the cultures were placed in an ELISA automatic reader to evaluate the release of nitric oxide. The production of nitric oxide for cement Sealer 26 was between 36.1 and 313.0 mumols, with a mean of 143.82±111.03mumols, while for the Endofill these values were significantly less (p=0.01), varying from 50.8 to 125.7mumols, with a mean of 80.33±28.42 mumols. In the positive and negative control groups the mean release of nitric oxide was of 162.75mumols and 4.42mumols, respectively. There was no significant difference between the positive control group and cement Sealer 26 (p>0.05). Therefore, the cement Sealer 26 caused significantly greater toxicity to the macrophages, possibly due to the components from the epoxy resin and formaldehyde release during polymerization. |
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Evaluation of cytotoxicity of two endodontic cements in a macrophage cultureRoot canal filling materialsNitric oxideMacrophagesCompared to gutta-percha, the endodontic cements are used in small quantity to seal root canals, but are indispensable to achieve hermetically sealed margins, where its biocompatibility depends on the sum of responses of each cell present in the periapical region. The object of this study was to evaluate the cytotoxicity of two endodontic cements, one based on epoxy resin (Sealer 26) and the other containing zinc oxide eugenol (Endofill) by using cultured peritoneal macrophages from Swiss mice to measure the induced production of nitric oxide. After solidification and pulverization, aliquots of 100mul of suspension containing 18mg/mL of the respective cements were added to 96-well tissue culture plates containing the tissue culture of macrophages at a concentration of 5.0X10(6) cells/ml. In the positive control group the cell culture was treated with 10mg/mL of lipopolyssaccharide from Escherichia coli 026:B6 and the cell culture alone represented the negative control. After 48 hours of incubation, at 37ºC, in 5% CO2, the cultures were placed in an ELISA automatic reader to evaluate the release of nitric oxide. The production of nitric oxide for cement Sealer 26 was between 36.1 and 313.0 mumols, with a mean of 143.82±111.03mumols, while for the Endofill these values were significantly less (p=0.01), varying from 50.8 to 125.7mumols, with a mean of 80.33±28.42 mumols. In the positive and negative control groups the mean release of nitric oxide was of 162.75mumols and 4.42mumols, respectively. There was no significant difference between the positive control group and cement Sealer 26 (p>0.05). Therefore, the cement Sealer 26 caused significantly greater toxicity to the macrophages, possibly due to the components from the epoxy resin and formaldehyde release during polymerization.Faculdade De Odontologia De Bauru - USP2005-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572005000300007Journal of Applied Oral Science v.13 n.3 2005reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S1678-77572005000300007info:eu-repo/semantics/openAccessQueiroz,Celso Emanoel de SouzaSoares,Janir AlvesLeonardo,Renato de ToledoCarlos,Iracilda ZeponiDinelli,Welingtoneng2005-10-24T00:00:00Zoai:scielo:S1678-77572005000300007Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2005-10-24T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Evaluation of cytotoxicity of two endodontic cements in a macrophage culture |
title |
Evaluation of cytotoxicity of two endodontic cements in a macrophage culture |
spellingShingle |
Evaluation of cytotoxicity of two endodontic cements in a macrophage culture Queiroz,Celso Emanoel de Souza Root canal filling materials Nitric oxide Macrophages |
title_short |
Evaluation of cytotoxicity of two endodontic cements in a macrophage culture |
title_full |
Evaluation of cytotoxicity of two endodontic cements in a macrophage culture |
title_fullStr |
Evaluation of cytotoxicity of two endodontic cements in a macrophage culture |
title_full_unstemmed |
Evaluation of cytotoxicity of two endodontic cements in a macrophage culture |
title_sort |
Evaluation of cytotoxicity of two endodontic cements in a macrophage culture |
author |
Queiroz,Celso Emanoel de Souza |
author_facet |
Queiroz,Celso Emanoel de Souza Soares,Janir Alves Leonardo,Renato de Toledo Carlos,Iracilda Zeponi Dinelli,Welington |
author_role |
author |
author2 |
Soares,Janir Alves Leonardo,Renato de Toledo Carlos,Iracilda Zeponi Dinelli,Welington |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Queiroz,Celso Emanoel de Souza Soares,Janir Alves Leonardo,Renato de Toledo Carlos,Iracilda Zeponi Dinelli,Welington |
dc.subject.por.fl_str_mv |
Root canal filling materials Nitric oxide Macrophages |
topic |
Root canal filling materials Nitric oxide Macrophages |
description |
Compared to gutta-percha, the endodontic cements are used in small quantity to seal root canals, but are indispensable to achieve hermetically sealed margins, where its biocompatibility depends on the sum of responses of each cell present in the periapical region. The object of this study was to evaluate the cytotoxicity of two endodontic cements, one based on epoxy resin (Sealer 26) and the other containing zinc oxide eugenol (Endofill) by using cultured peritoneal macrophages from Swiss mice to measure the induced production of nitric oxide. After solidification and pulverization, aliquots of 100mul of suspension containing 18mg/mL of the respective cements were added to 96-well tissue culture plates containing the tissue culture of macrophages at a concentration of 5.0X10(6) cells/ml. In the positive control group the cell culture was treated with 10mg/mL of lipopolyssaccharide from Escherichia coli 026:B6 and the cell culture alone represented the negative control. After 48 hours of incubation, at 37ºC, in 5% CO2, the cultures were placed in an ELISA automatic reader to evaluate the release of nitric oxide. The production of nitric oxide for cement Sealer 26 was between 36.1 and 313.0 mumols, with a mean of 143.82±111.03mumols, while for the Endofill these values were significantly less (p=0.01), varying from 50.8 to 125.7mumols, with a mean of 80.33±28.42 mumols. In the positive and negative control groups the mean release of nitric oxide was of 162.75mumols and 4.42mumols, respectively. There was no significant difference between the positive control group and cement Sealer 26 (p>0.05). Therefore, the cement Sealer 26 caused significantly greater toxicity to the macrophages, possibly due to the components from the epoxy resin and formaldehyde release during polymerization. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572005000300007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572005000300007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1678-77572005000300007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science v.13 n.3 2005 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1748936433905696768 |