Evaluation of two morphometric methods of bone loss percentages caused by periodontitis in rats in different locations
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/3832 |
Resumo: | ABSTRACT OBJECTIVE: The present study evaluated morphometrically bone loss percentages in experimental periodontitis in rats, comparing different locations (lingual mandible, palatal maxilla and buccal maxilla) and two evaluation methods (distance and area methods). MATERIAL AND METHODS: Ligatures were placed around the maxillary right second molar and around the mandibular right first molar in 14 female Wistar rats. The contralateral molars served as intragroup controls. After 4 weeks, the rats were sacrificed and their mandible and maxilla were removed. The specimens were dissected and stained with methylene blue dye. Bone loss was evaluated by two different methods on the surfaces of the defleshed jaw. In the first method, the distance from the cementoenamel junction (CEJ) to the alveolar bone crest was measured in the roots of teeth associated with ligature. In the second method, the area of bone loss was determined using the alveolar tissue bone, CEJ and the proximal region of roots associated with the ligature as reference. The data were converted to bone loss percentages caused by ligature: (ligated - unligated) x 100/ligated. RESULTS: When comparing the distance and area methods, no statistically significant difference was observed (p>0.05). Both methodologies indicated that the maxilla presented greater bone loss than the mandible and it was more accentuated on the buccal side than on the palatal side (p |
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Journal of applied oral science (Online) |
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Evaluation of two morphometric methods of bone loss percentages caused by periodontitis in rats in different locations Experimental studiesMethodologyPeriodontal diseasesRats ABSTRACT OBJECTIVE: The present study evaluated morphometrically bone loss percentages in experimental periodontitis in rats, comparing different locations (lingual mandible, palatal maxilla and buccal maxilla) and two evaluation methods (distance and area methods). MATERIAL AND METHODS: Ligatures were placed around the maxillary right second molar and around the mandibular right first molar in 14 female Wistar rats. The contralateral molars served as intragroup controls. After 4 weeks, the rats were sacrificed and their mandible and maxilla were removed. The specimens were dissected and stained with methylene blue dye. Bone loss was evaluated by two different methods on the surfaces of the defleshed jaw. In the first method, the distance from the cementoenamel junction (CEJ) to the alveolar bone crest was measured in the roots of teeth associated with ligature. In the second method, the area of bone loss was determined using the alveolar tissue bone, CEJ and the proximal region of roots associated with the ligature as reference. The data were converted to bone loss percentages caused by ligature: (ligated - unligated) x 100/ligated. RESULTS: When comparing the distance and area methods, no statistically significant difference was observed (p>0.05). Both methodologies indicated that the maxilla presented greater bone loss than the mandible and it was more accentuated on the buccal side than on the palatal side (pUniversidade de São Paulo. Faculdade de Odontologia de Bauru2010-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/383210.1590/S1678-77572010000500011Journal of Applied Oral Science; Vol. 18 No. 5 (2010); 493-497 Journal of Applied Oral Science; Vol. 18 Núm. 5 (2010); 493-497 Journal of Applied Oral Science; v. 18 n. 5 (2010); 493-497 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3832/4522Copyright (c) 2010 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessSouza, Daniela Martins dePrado, Fernanda de AlmeidaPrado, Marcela de AlmeidaRocha, Rosilene Fernandes daCarvalho, Yasmin Rodarte de2012-04-27T12:10:54Zoai:revistas.usp.br:article/3832Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T12:10:54Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Evaluation of two morphometric methods of bone loss percentages caused by periodontitis in rats in different locations |
title |
Evaluation of two morphometric methods of bone loss percentages caused by periodontitis in rats in different locations |
spellingShingle |
Evaluation of two morphometric methods of bone loss percentages caused by periodontitis in rats in different locations Souza, Daniela Martins de Experimental studies Methodology Periodontal diseases Rats |
title_short |
Evaluation of two morphometric methods of bone loss percentages caused by periodontitis in rats in different locations |
title_full |
Evaluation of two morphometric methods of bone loss percentages caused by periodontitis in rats in different locations |
title_fullStr |
Evaluation of two morphometric methods of bone loss percentages caused by periodontitis in rats in different locations |
title_full_unstemmed |
Evaluation of two morphometric methods of bone loss percentages caused by periodontitis in rats in different locations |
title_sort |
Evaluation of two morphometric methods of bone loss percentages caused by periodontitis in rats in different locations |
author |
Souza, Daniela Martins de |
author_facet |
Souza, Daniela Martins de Prado, Fernanda de Almeida Prado, Marcela de Almeida Rocha, Rosilene Fernandes da Carvalho, Yasmin Rodarte de |
author_role |
author |
author2 |
Prado, Fernanda de Almeida Prado, Marcela de Almeida Rocha, Rosilene Fernandes da Carvalho, Yasmin Rodarte de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Souza, Daniela Martins de Prado, Fernanda de Almeida Prado, Marcela de Almeida Rocha, Rosilene Fernandes da Carvalho, Yasmin Rodarte de |
dc.subject.por.fl_str_mv |
Experimental studies Methodology Periodontal diseases Rats |
topic |
Experimental studies Methodology Periodontal diseases Rats |
description |
ABSTRACT OBJECTIVE: The present study evaluated morphometrically bone loss percentages in experimental periodontitis in rats, comparing different locations (lingual mandible, palatal maxilla and buccal maxilla) and two evaluation methods (distance and area methods). MATERIAL AND METHODS: Ligatures were placed around the maxillary right second molar and around the mandibular right first molar in 14 female Wistar rats. The contralateral molars served as intragroup controls. After 4 weeks, the rats were sacrificed and their mandible and maxilla were removed. The specimens were dissected and stained with methylene blue dye. Bone loss was evaluated by two different methods on the surfaces of the defleshed jaw. In the first method, the distance from the cementoenamel junction (CEJ) to the alveolar bone crest was measured in the roots of teeth associated with ligature. In the second method, the area of bone loss was determined using the alveolar tissue bone, CEJ and the proximal region of roots associated with the ligature as reference. The data were converted to bone loss percentages caused by ligature: (ligated - unligated) x 100/ligated. RESULTS: When comparing the distance and area methods, no statistically significant difference was observed (p>0.05). Both methodologies indicated that the maxilla presented greater bone loss than the mandible and it was more accentuated on the buccal side than on the palatal side (p |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3832 10.1590/S1678-77572010000500011 |
url |
https://www.revistas.usp.br/jaos/article/view/3832 |
identifier_str_mv |
10.1590/S1678-77572010000500011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3832/4522 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2010 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2010 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 18 No. 5 (2010); 493-497 Journal of Applied Oral Science; Vol. 18 Núm. 5 (2010); 493-497 Journal of Applied Oral Science; v. 18 n. 5 (2010); 493-497 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221676095406080 |