Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/187110 |
Resumo: | Objective: The aim of this study was to evaluate whether probiotics multi-strain formula affects the development of apical periodontitis (AP) induced in rats. Methodology: 16 Wistar rats were divided in two groups (n=8): rats with AP fed with regular diet (Control-C (CG)); rats with AP, fed with regular diet and supplemented with multi-strain formula (one billion colony-forming units (CFU)): GNC Probiotic Complex (PCG) ( Lactobacillus acidophilus, Lactobacillus salivaris, Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium bifidum, Bifidobacterium animalis subs. lactis and Streptococcus thermofilus ). AP was induced in the upper and lower first molars by dental pulp exposure to the oral environment. PCG was administered orally through gavage for 30 days during the AP development. After this period the animals were euthanized and the mandibles were removed and processed for histologic analysis, and immunochemical assays for interleukin (IL)-6, IL-10, IL-1β, RANKL, OPG, and TRAP. The Mann–Whitney U test and Student’s t test were performed (P<.05). Results: The CG showed more intense inflammatory infiltrate than the PCG group (P<.05). IL-1β, IL 6 and RANKL decreased in the PCG group compared with CG (P<.05). The IL-10 level increased in the PCG group (P<.05). The OPG level was similar in both groups (P>.05). The number of mature osteoclasts (TRAP-positive multinucleated cells) was lower in PCG group when compared to the CG (P<.05). Conclusion: Probiotic Complex modulates inflammation and bone resorption in apical periodontitis. |
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Journal of applied oral science (Online) |
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Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitisApical periodontitisInflammatory infiltrateInterleukinBone resorptionProbioticsObjective: The aim of this study was to evaluate whether probiotics multi-strain formula affects the development of apical periodontitis (AP) induced in rats. Methodology: 16 Wistar rats were divided in two groups (n=8): rats with AP fed with regular diet (Control-C (CG)); rats with AP, fed with regular diet and supplemented with multi-strain formula (one billion colony-forming units (CFU)): GNC Probiotic Complex (PCG) ( Lactobacillus acidophilus, Lactobacillus salivaris, Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium bifidum, Bifidobacterium animalis subs. lactis and Streptococcus thermofilus ). AP was induced in the upper and lower first molars by dental pulp exposure to the oral environment. PCG was administered orally through gavage for 30 days during the AP development. After this period the animals were euthanized and the mandibles were removed and processed for histologic analysis, and immunochemical assays for interleukin (IL)-6, IL-10, IL-1β, RANKL, OPG, and TRAP. The Mann–Whitney U test and Student’s t test were performed (P<.05). Results: The CG showed more intense inflammatory infiltrate than the PCG group (P<.05). IL-1β, IL 6 and RANKL decreased in the PCG group compared with CG (P<.05). The IL-10 level increased in the PCG group (P<.05). The OPG level was similar in both groups (P>.05). The number of mature osteoclasts (TRAP-positive multinucleated cells) was lower in PCG group when compared to the CG (P<.05). Conclusion: Probiotic Complex modulates inflammation and bone resorption in apical periodontitis.Universidade de São Paulo. Faculdade de Odontologia de Bauru2021-06-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/18711010.1590/1678-7757-2020-0483Journal of Applied Oral Science; Vol. 29 (2021); e20210483Journal of Applied Oral Science; Vol. 29 (2021); e20210483Journal of Applied Oral Science; v. 29 (2021); e202104831678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/187110/172851Copyright (c) 2021 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCosme-Silva, Leopoldo Dal-Fabro, Renan Cintra, Luciano Tavares Angelo Ervolino, Edilson Prado, Alana Sant’Ana do Oliveira, Daniel Pinto de Marcelos, Priscylla Gonçalves Correia Leite de Gomes-Filho, João Eduardo 2021-06-14T18:07:40Zoai:revistas.usp.br:article/187110Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2021-06-14T18:07:40Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis |
title |
Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis |
spellingShingle |
Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis Cosme-Silva, Leopoldo Apical periodontitis Inflammatory infiltrate Interleukin Bone resorption Probiotics |
title_short |
Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis |
title_full |
Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis |
title_fullStr |
Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis |
title_full_unstemmed |
Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis |
title_sort |
Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis |
author |
Cosme-Silva, Leopoldo |
author_facet |
Cosme-Silva, Leopoldo Dal-Fabro, Renan Cintra, Luciano Tavares Angelo Ervolino, Edilson Prado, Alana Sant’Ana do Oliveira, Daniel Pinto de Marcelos, Priscylla Gonçalves Correia Leite de Gomes-Filho, João Eduardo |
author_role |
author |
author2 |
Dal-Fabro, Renan Cintra, Luciano Tavares Angelo Ervolino, Edilson Prado, Alana Sant’Ana do Oliveira, Daniel Pinto de Marcelos, Priscylla Gonçalves Correia Leite de Gomes-Filho, João Eduardo |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Cosme-Silva, Leopoldo Dal-Fabro, Renan Cintra, Luciano Tavares Angelo Ervolino, Edilson Prado, Alana Sant’Ana do Oliveira, Daniel Pinto de Marcelos, Priscylla Gonçalves Correia Leite de Gomes-Filho, João Eduardo |
dc.subject.por.fl_str_mv |
Apical periodontitis Inflammatory infiltrate Interleukin Bone resorption Probiotics |
topic |
Apical periodontitis Inflammatory infiltrate Interleukin Bone resorption Probiotics |
description |
Objective: The aim of this study was to evaluate whether probiotics multi-strain formula affects the development of apical periodontitis (AP) induced in rats. Methodology: 16 Wistar rats were divided in two groups (n=8): rats with AP fed with regular diet (Control-C (CG)); rats with AP, fed with regular diet and supplemented with multi-strain formula (one billion colony-forming units (CFU)): GNC Probiotic Complex (PCG) ( Lactobacillus acidophilus, Lactobacillus salivaris, Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium bifidum, Bifidobacterium animalis subs. lactis and Streptococcus thermofilus ). AP was induced in the upper and lower first molars by dental pulp exposure to the oral environment. PCG was administered orally through gavage for 30 days during the AP development. After this period the animals were euthanized and the mandibles were removed and processed for histologic analysis, and immunochemical assays for interleukin (IL)-6, IL-10, IL-1β, RANKL, OPG, and TRAP. The Mann–Whitney U test and Student’s t test were performed (P<.05). Results: The CG showed more intense inflammatory infiltrate than the PCG group (P<.05). IL-1β, IL 6 and RANKL decreased in the PCG group compared with CG (P<.05). The IL-10 level increased in the PCG group (P<.05). The OPG level was similar in both groups (P>.05). The number of mature osteoclasts (TRAP-positive multinucleated cells) was lower in PCG group when compared to the CG (P<.05). Conclusion: Probiotic Complex modulates inflammation and bone resorption in apical periodontitis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/187110 10.1590/1678-7757-2020-0483 |
url |
https://www.revistas.usp.br/jaos/article/view/187110 |
identifier_str_mv |
10.1590/1678-7757-2020-0483 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/187110/172851 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 29 (2021); e20210483 Journal of Applied Oral Science; Vol. 29 (2021); e20210483 Journal of Applied Oral Science; v. 29 (2021); e20210483 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221681233428480 |