Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis

Detalhes bibliográficos
Autor(a) principal: Cosme-Silva, Leopoldo
Data de Publicação: 2021
Outros Autores: Dal-Fabro, Renan, Cintra, Luciano Tavares Angelo, Ervolino, Edilson, Prado, Alana Sant’Ana do, Oliveira, Daniel Pinto de, Marcelos, Priscylla Gonçalves Correia Leite de, Gomes-Filho, João Eduardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/187110
Resumo: Objective: The aim of this study was to evaluate whether probiotics multi-strain formula affects the development of apical periodontitis (AP) induced in rats. Methodology: 16 Wistar rats were divided in two groups (n=8): rats with AP fed with regular diet (Control-C (CG)); rats with AP, fed with regular diet and supplemented with multi-strain formula (one billion colony-forming units (CFU)): GNC Probiotic Complex (PCG) ( Lactobacillus acidophilus, Lactobacillus salivaris, Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium bifidum, Bifidobacterium animalis subs. lactis and Streptococcus thermofilus ). AP was induced in the upper and lower first molars by dental pulp exposure to the oral environment. PCG was administered orally through gavage for 30 days during the AP development. After this period the animals were euthanized and the mandibles were removed and processed for histologic analysis, and immunochemical assays for interleukin (IL)-6, IL-10, IL-1β, RANKL, OPG, and TRAP. The Mann–Whitney U test and Student’s t test were performed (P<.05). Results:  The CG showed more intense inflammatory infiltrate than the PCG group (P<.05). IL-1β, IL 6 and RANKL decreased in the PCG group compared with CG (P<.05). The IL-10 level increased in the PCG group (P<.05). The OPG level was similar in both groups (P>.05). The number of mature osteoclasts (TRAP-positive multinucleated cells) was lower in PCG group when compared to the CG (P<.05). Conclusion: Probiotic Complex modulates inflammation and bone resorption in apical periodontitis.
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spelling Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitisApical periodontitisInflammatory infiltrateInterleukinBone resorptionProbioticsObjective: The aim of this study was to evaluate whether probiotics multi-strain formula affects the development of apical periodontitis (AP) induced in rats. Methodology: 16 Wistar rats were divided in two groups (n=8): rats with AP fed with regular diet (Control-C (CG)); rats with AP, fed with regular diet and supplemented with multi-strain formula (one billion colony-forming units (CFU)): GNC Probiotic Complex (PCG) ( Lactobacillus acidophilus, Lactobacillus salivaris, Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium bifidum, Bifidobacterium animalis subs. lactis and Streptococcus thermofilus ). AP was induced in the upper and lower first molars by dental pulp exposure to the oral environment. PCG was administered orally through gavage for 30 days during the AP development. After this period the animals were euthanized and the mandibles were removed and processed for histologic analysis, and immunochemical assays for interleukin (IL)-6, IL-10, IL-1β, RANKL, OPG, and TRAP. The Mann–Whitney U test and Student’s t test were performed (P<.05). Results:  The CG showed more intense inflammatory infiltrate than the PCG group (P<.05). IL-1β, IL 6 and RANKL decreased in the PCG group compared with CG (P<.05). The IL-10 level increased in the PCG group (P<.05). The OPG level was similar in both groups (P>.05). The number of mature osteoclasts (TRAP-positive multinucleated cells) was lower in PCG group when compared to the CG (P<.05). Conclusion: Probiotic Complex modulates inflammation and bone resorption in apical periodontitis.Universidade de São Paulo. Faculdade de Odontologia de Bauru2021-06-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/18711010.1590/1678-7757-2020-0483Journal of Applied Oral Science; Vol. 29 (2021); e20210483Journal of Applied Oral Science; Vol. 29 (2021); e20210483Journal of Applied Oral Science; v. 29 (2021); e202104831678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/187110/172851Copyright (c) 2021 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCosme-Silva, Leopoldo Dal-Fabro, Renan Cintra, Luciano Tavares Angelo Ervolino, Edilson Prado, Alana Sant’Ana do Oliveira, Daniel Pinto de Marcelos, Priscylla Gonçalves Correia Leite de Gomes-Filho, João Eduardo 2021-06-14T18:07:40Zoai:revistas.usp.br:article/187110Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2021-06-14T18:07:40Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis
title Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis
spellingShingle Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis
Cosme-Silva, Leopoldo
Apical periodontitis
Inflammatory infiltrate
Interleukin
Bone resorption
Probiotics
title_short Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis
title_full Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis
title_fullStr Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis
title_full_unstemmed Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis
title_sort Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis
author Cosme-Silva, Leopoldo
author_facet Cosme-Silva, Leopoldo
Dal-Fabro, Renan
Cintra, Luciano Tavares Angelo
Ervolino, Edilson
Prado, Alana Sant’Ana do
Oliveira, Daniel Pinto de
Marcelos, Priscylla Gonçalves Correia Leite de
Gomes-Filho, João Eduardo
author_role author
author2 Dal-Fabro, Renan
Cintra, Luciano Tavares Angelo
Ervolino, Edilson
Prado, Alana Sant’Ana do
Oliveira, Daniel Pinto de
Marcelos, Priscylla Gonçalves Correia Leite de
Gomes-Filho, João Eduardo
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cosme-Silva, Leopoldo
Dal-Fabro, Renan
Cintra, Luciano Tavares Angelo
Ervolino, Edilson
Prado, Alana Sant’Ana do
Oliveira, Daniel Pinto de
Marcelos, Priscylla Gonçalves Correia Leite de
Gomes-Filho, João Eduardo
dc.subject.por.fl_str_mv Apical periodontitis
Inflammatory infiltrate
Interleukin
Bone resorption
Probiotics
topic Apical periodontitis
Inflammatory infiltrate
Interleukin
Bone resorption
Probiotics
description Objective: The aim of this study was to evaluate whether probiotics multi-strain formula affects the development of apical periodontitis (AP) induced in rats. Methodology: 16 Wistar rats were divided in two groups (n=8): rats with AP fed with regular diet (Control-C (CG)); rats with AP, fed with regular diet and supplemented with multi-strain formula (one billion colony-forming units (CFU)): GNC Probiotic Complex (PCG) ( Lactobacillus acidophilus, Lactobacillus salivaris, Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium bifidum, Bifidobacterium animalis subs. lactis and Streptococcus thermofilus ). AP was induced in the upper and lower first molars by dental pulp exposure to the oral environment. PCG was administered orally through gavage for 30 days during the AP development. After this period the animals were euthanized and the mandibles were removed and processed for histologic analysis, and immunochemical assays for interleukin (IL)-6, IL-10, IL-1β, RANKL, OPG, and TRAP. The Mann–Whitney U test and Student’s t test were performed (P<.05). Results:  The CG showed more intense inflammatory infiltrate than the PCG group (P<.05). IL-1β, IL 6 and RANKL decreased in the PCG group compared with CG (P<.05). The IL-10 level increased in the PCG group (P<.05). The OPG level was similar in both groups (P>.05). The number of mature osteoclasts (TRAP-positive multinucleated cells) was lower in PCG group when compared to the CG (P<.05). Conclusion: Probiotic Complex modulates inflammation and bone resorption in apical periodontitis.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-14
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/187110
10.1590/1678-7757-2020-0483
url https://www.revistas.usp.br/jaos/article/view/187110
identifier_str_mv 10.1590/1678-7757-2020-0483
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/187110/172851
dc.rights.driver.fl_str_mv Copyright (c) 2021 Journal of Applied Oral Science
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Journal of Applied Oral Science
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 29 (2021); e20210483
Journal of Applied Oral Science; Vol. 29 (2021); e20210483
Journal of Applied Oral Science; v. 29 (2021); e20210483
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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