Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572015000500467 |
Resumo: | Mineral Trioxide Aggregate (MTA) is a calcium silicate-based material. New sealers have been developed based on calcium silicate as MTA Fillapex and MTA Plus. Objective The aim of this study was to evaluate biocompatibility and bioactivity of these two calcium silicate-based sealers in culture of human dental pulp cells (hDPCs). Material and Methods The cells were isolated from third molars extracted from a 16-year-old patient. Pulp tissue was sectioned into fragments with approximately 1 mm3 and kept in supplemented medium to obtain hDPCs adherent cultures. Cell characterization assays were performed to prove the osteogenic potential. The evaluated materials were: MTA Plus (MTAP); MTA Fillapex (MTAF) and FillCanal (FC). Biocompatibility was evaluated with MTT and Neutral Red (NR) assays, after hDPCs exposure for 24 h to different dilutions of each sealer extract (1:2, 1:3 and 1:4). Unexposed cells were the positive control (CT). Bioactivity was assessed by alkaline phosphatase (ALP) enzymatic assay in cells exposed for one and three days to sealer extracts (1:4 dilution). All data were analyzed by ANOVA and Tukey post-test (p≤0.05%). Results MTT and NR results showed suitable cell viability rates for MTAP at all dilutions (90-135%). Cells exposed to MTAF and FC (1:2 and 1:4 dilutions) showed significant low viability rate when compared to CT in MTT. The NR results demonstrated cell viability for all materials tested. In MTAP group, the cells ALP activity was similar to CT in one and three days of exposure to the material. MTAF and FC groups demonstrated a decrease in ALP activity when compared to CT at both periods of cell exposure. Conclusions The hDPCs were suitable for the evaluation of new endodontic materials in vitro. MTAP may be considered a promising material for endodontic treatments. |
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Journal of applied oral science (Online) |
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Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cellsDental pulpSilicate cementCell viabilityAlkaline phosphataseMineral Trioxide Aggregate (MTA) is a calcium silicate-based material. New sealers have been developed based on calcium silicate as MTA Fillapex and MTA Plus. Objective The aim of this study was to evaluate biocompatibility and bioactivity of these two calcium silicate-based sealers in culture of human dental pulp cells (hDPCs). Material and Methods The cells were isolated from third molars extracted from a 16-year-old patient. Pulp tissue was sectioned into fragments with approximately 1 mm3 and kept in supplemented medium to obtain hDPCs adherent cultures. Cell characterization assays were performed to prove the osteogenic potential. The evaluated materials were: MTA Plus (MTAP); MTA Fillapex (MTAF) and FillCanal (FC). Biocompatibility was evaluated with MTT and Neutral Red (NR) assays, after hDPCs exposure for 24 h to different dilutions of each sealer extract (1:2, 1:3 and 1:4). Unexposed cells were the positive control (CT). Bioactivity was assessed by alkaline phosphatase (ALP) enzymatic assay in cells exposed for one and three days to sealer extracts (1:4 dilution). All data were analyzed by ANOVA and Tukey post-test (p≤0.05%). Results MTT and NR results showed suitable cell viability rates for MTAP at all dilutions (90-135%). Cells exposed to MTAF and FC (1:2 and 1:4 dilutions) showed significant low viability rate when compared to CT in MTT. The NR results demonstrated cell viability for all materials tested. In MTAP group, the cells ALP activity was similar to CT in one and three days of exposure to the material. MTAF and FC groups demonstrated a decrease in ALP activity when compared to CT at both periods of cell exposure. Conclusions The hDPCs were suitable for the evaluation of new endodontic materials in vitro. MTAP may be considered a promising material for endodontic treatments.Faculdade De Odontologia De Bauru - USP2015-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572015000500467Journal of Applied Oral Science v.23 n.5 2015reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-775720150170info:eu-repo/semantics/openAccessMESTIERI,Leticia BoldrinGOMES-CORNÉLIO,Ana LíviaRODRIGUES,Elisandra MárciaSALLES,Loise PedrosaBOSSO-MARTELO,RobertaGUERREIRO-TANOMARU,Juliane MariaTANOMARU-FILHO,Márioeng2016-05-12T00:00:00Zoai:scielo:S1678-77572015000500467Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2016-05-12T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells |
title |
Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells |
spellingShingle |
Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells MESTIERI,Leticia Boldrin Dental pulp Silicate cement Cell viability Alkaline phosphatase |
title_short |
Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells |
title_full |
Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells |
title_fullStr |
Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells |
title_full_unstemmed |
Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells |
title_sort |
Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells |
author |
MESTIERI,Leticia Boldrin |
author_facet |
MESTIERI,Leticia Boldrin GOMES-CORNÉLIO,Ana Lívia RODRIGUES,Elisandra Márcia SALLES,Loise Pedrosa BOSSO-MARTELO,Roberta GUERREIRO-TANOMARU,Juliane Maria TANOMARU-FILHO,Mário |
author_role |
author |
author2 |
GOMES-CORNÉLIO,Ana Lívia RODRIGUES,Elisandra Márcia SALLES,Loise Pedrosa BOSSO-MARTELO,Roberta GUERREIRO-TANOMARU,Juliane Maria TANOMARU-FILHO,Mário |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
MESTIERI,Leticia Boldrin GOMES-CORNÉLIO,Ana Lívia RODRIGUES,Elisandra Márcia SALLES,Loise Pedrosa BOSSO-MARTELO,Roberta GUERREIRO-TANOMARU,Juliane Maria TANOMARU-FILHO,Mário |
dc.subject.por.fl_str_mv |
Dental pulp Silicate cement Cell viability Alkaline phosphatase |
topic |
Dental pulp Silicate cement Cell viability Alkaline phosphatase |
description |
Mineral Trioxide Aggregate (MTA) is a calcium silicate-based material. New sealers have been developed based on calcium silicate as MTA Fillapex and MTA Plus. Objective The aim of this study was to evaluate biocompatibility and bioactivity of these two calcium silicate-based sealers in culture of human dental pulp cells (hDPCs). Material and Methods The cells were isolated from third molars extracted from a 16-year-old patient. Pulp tissue was sectioned into fragments with approximately 1 mm3 and kept in supplemented medium to obtain hDPCs adherent cultures. Cell characterization assays were performed to prove the osteogenic potential. The evaluated materials were: MTA Plus (MTAP); MTA Fillapex (MTAF) and FillCanal (FC). Biocompatibility was evaluated with MTT and Neutral Red (NR) assays, after hDPCs exposure for 24 h to different dilutions of each sealer extract (1:2, 1:3 and 1:4). Unexposed cells were the positive control (CT). Bioactivity was assessed by alkaline phosphatase (ALP) enzymatic assay in cells exposed for one and three days to sealer extracts (1:4 dilution). All data were analyzed by ANOVA and Tukey post-test (p≤0.05%). Results MTT and NR results showed suitable cell viability rates for MTAP at all dilutions (90-135%). Cells exposed to MTAF and FC (1:2 and 1:4 dilutions) showed significant low viability rate when compared to CT in MTT. The NR results demonstrated cell viability for all materials tested. In MTAP group, the cells ALP activity was similar to CT in one and three days of exposure to the material. MTAF and FC groups demonstrated a decrease in ALP activity when compared to CT at both periods of cell exposure. Conclusions The hDPCs were suitable for the evaluation of new endodontic materials in vitro. MTAP may be considered a promising material for endodontic treatments. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572015000500467 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572015000500467 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-775720150170 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science v.23 n.5 2015 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
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1748936438593880064 |