Impact of calcium aluminate cement with additives on dental pulp-derived cells
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/187120 |
Resumo: | Calcium aluminate cement (CAC) has been highlighted as a promising alternative for endodontic use aiming at periapical tissue repair. However, its effects on dental pulp cells have been poorly explored. Objective: This study assessed the impact of calcium chloride (CaCl2) and bismuth oxide (Bi2O3) or zinc oxide (ZnO) additives on odontoblast cell response to CAC. Methodology: MDPC-23 cells were exposed for up to 14 d: 1) CAC with 2.8% CaCl2 and 25% ZnO (CACz); 2) CAC with 2.8% CaCl2 and 25% Bi2O3 (CACb); 3) CAC with 10% CaCl2 and 25% Bi2O3 (CACb+); or 4) mineral trioxide aggregate (MTA), placed on inserts. Non-exposed cultures served as control. Cell morphology, cell viability, gene expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), and dentin matrix protein 1 (DMP-1), ALP activity, and extracellular matrix mineralization were evaluated. Data were compared using ANOVA (α=5%). Results: Lower cell density was detected only for MTA and CACb+ compared with Control, with areas showing reduced cell spreading. Cell viability was similar among groups at days one and three (p>0.05). CACb+ and MTA showed the lowest cell viability values at day seven (p>0.05). CACb and CACb+ promoted higher ALP and BSP expression compared with CACz (p<0.05); despite that, all cements supported ALP activity. Matrix mineralization were enhanced in CACb+ and MTA. Conclusion: In conclusion, CAC with Bi2O3, but not with ZnO, supported the expression of odontoblastic phenotype, but only the composition with 10% CaCl2 promoted mineralized matrix formation, rendering it suitable for dentin-pulp complex repair. |
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oai:revistas.usp.br:article/187120 |
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USP-17 |
network_name_str |
Journal of applied oral science (Online) |
repository_id_str |
|
spelling |
Impact of calcium aluminate cement with additives on dental pulp-derived cellsOdontoblastsRoot canal filling materialsDental pulp cappingCalcium chlorideGene expressionCalcium aluminate cement (CAC) has been highlighted as a promising alternative for endodontic use aiming at periapical tissue repair. However, its effects on dental pulp cells have been poorly explored. Objective: This study assessed the impact of calcium chloride (CaCl2) and bismuth oxide (Bi2O3) or zinc oxide (ZnO) additives on odontoblast cell response to CAC. Methodology: MDPC-23 cells were exposed for up to 14 d: 1) CAC with 2.8% CaCl2 and 25% ZnO (CACz); 2) CAC with 2.8% CaCl2 and 25% Bi2O3 (CACb); 3) CAC with 10% CaCl2 and 25% Bi2O3 (CACb+); or 4) mineral trioxide aggregate (MTA), placed on inserts. Non-exposed cultures served as control. Cell morphology, cell viability, gene expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), and dentin matrix protein 1 (DMP-1), ALP activity, and extracellular matrix mineralization were evaluated. Data were compared using ANOVA (α=5%). Results: Lower cell density was detected only for MTA and CACb+ compared with Control, with areas showing reduced cell spreading. Cell viability was similar among groups at days one and three (p>0.05). CACb+ and MTA showed the lowest cell viability values at day seven (p>0.05). CACb and CACb+ promoted higher ALP and BSP expression compared with CACz (p<0.05); despite that, all cements supported ALP activity. Matrix mineralization were enhanced in CACb+ and MTA. Conclusion: In conclusion, CAC with Bi2O3, but not with ZnO, supported the expression of odontoblastic phenotype, but only the composition with 10% CaCl2 promoted mineralized matrix formation, rendering it suitable for dentin-pulp complex repair.Universidade de São Paulo. Faculdade de Odontologia de Bauru2021-06-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/18712010.1590/1678-7757-2019-0105 Journal of Applied Oral Science; Vol. 28 (2020); e20190105Journal of Applied Oral Science; Vol. 28 (2020); e20190105Journal of Applied Oral Science; v. 28 (2020); e201901051678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/187120/172873Copyright (c) 2021 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMessias, Nadyne Saab Grisote, Gabriela Martorano, Antonio Secco Fernandes, Roger Rodrigo Oliveira, Ivone Regina de Bombonato-Prado, Karina Fittipaldi Oliveira, Paulo Tambasco de Castro-Raucci, Larissa Moreira Spinola de 2021-06-14T17:33:06Zoai:revistas.usp.br:article/187120Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2021-06-14T17:33:06Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Impact of calcium aluminate cement with additives on dental pulp-derived cells |
title |
Impact of calcium aluminate cement with additives on dental pulp-derived cells |
spellingShingle |
Impact of calcium aluminate cement with additives on dental pulp-derived cells Messias, Nadyne Saab Odontoblasts Root canal filling materials Dental pulp capping Calcium chloride Gene expression |
title_short |
Impact of calcium aluminate cement with additives on dental pulp-derived cells |
title_full |
Impact of calcium aluminate cement with additives on dental pulp-derived cells |
title_fullStr |
Impact of calcium aluminate cement with additives on dental pulp-derived cells |
title_full_unstemmed |
Impact of calcium aluminate cement with additives on dental pulp-derived cells |
title_sort |
Impact of calcium aluminate cement with additives on dental pulp-derived cells |
author |
Messias, Nadyne Saab |
author_facet |
Messias, Nadyne Saab Grisote, Gabriela Martorano, Antonio Secco Fernandes, Roger Rodrigo Oliveira, Ivone Regina de Bombonato-Prado, Karina Fittipaldi Oliveira, Paulo Tambasco de Castro-Raucci, Larissa Moreira Spinola de |
author_role |
author |
author2 |
Grisote, Gabriela Martorano, Antonio Secco Fernandes, Roger Rodrigo Oliveira, Ivone Regina de Bombonato-Prado, Karina Fittipaldi Oliveira, Paulo Tambasco de Castro-Raucci, Larissa Moreira Spinola de |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Messias, Nadyne Saab Grisote, Gabriela Martorano, Antonio Secco Fernandes, Roger Rodrigo Oliveira, Ivone Regina de Bombonato-Prado, Karina Fittipaldi Oliveira, Paulo Tambasco de Castro-Raucci, Larissa Moreira Spinola de |
dc.subject.por.fl_str_mv |
Odontoblasts Root canal filling materials Dental pulp capping Calcium chloride Gene expression |
topic |
Odontoblasts Root canal filling materials Dental pulp capping Calcium chloride Gene expression |
description |
Calcium aluminate cement (CAC) has been highlighted as a promising alternative for endodontic use aiming at periapical tissue repair. However, its effects on dental pulp cells have been poorly explored. Objective: This study assessed the impact of calcium chloride (CaCl2) and bismuth oxide (Bi2O3) or zinc oxide (ZnO) additives on odontoblast cell response to CAC. Methodology: MDPC-23 cells were exposed for up to 14 d: 1) CAC with 2.8% CaCl2 and 25% ZnO (CACz); 2) CAC with 2.8% CaCl2 and 25% Bi2O3 (CACb); 3) CAC with 10% CaCl2 and 25% Bi2O3 (CACb+); or 4) mineral trioxide aggregate (MTA), placed on inserts. Non-exposed cultures served as control. Cell morphology, cell viability, gene expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), and dentin matrix protein 1 (DMP-1), ALP activity, and extracellular matrix mineralization were evaluated. Data were compared using ANOVA (α=5%). Results: Lower cell density was detected only for MTA and CACb+ compared with Control, with areas showing reduced cell spreading. Cell viability was similar among groups at days one and three (p>0.05). CACb+ and MTA showed the lowest cell viability values at day seven (p>0.05). CACb and CACb+ promoted higher ALP and BSP expression compared with CACz (p<0.05); despite that, all cements supported ALP activity. Matrix mineralization were enhanced in CACb+ and MTA. Conclusion: In conclusion, CAC with Bi2O3, but not with ZnO, supported the expression of odontoblastic phenotype, but only the composition with 10% CaCl2 promoted mineralized matrix formation, rendering it suitable for dentin-pulp complex repair. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/187120 10.1590/1678-7757-2019-0105 |
url |
https://www.revistas.usp.br/jaos/article/view/187120 |
identifier_str_mv |
10.1590/1678-7757-2019-0105 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/187120/172873 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 28 (2020); e20190105 Journal of Applied Oral Science; Vol. 28 (2020); e20190105 Journal of Applied Oral Science; v. 28 (2020); e20190105 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221681238671360 |