Interleukin-1 beta and interleukin-8 in healthy and inflamed dental pulps

Detalhes bibliográficos
Autor(a) principal: Silva, Alessandra Cecília Oliveira
Data de Publicação: 2009
Outros Autores: Faria, Miriam Rubio, Fontes, Alexandra, Campos, Marcia Sampaio, Cavalcanti, Bruno Neves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/3731
Resumo: After aggression to the dental pulp, some cells produce cytokines in order to start and control the inflammatory process. Among these cytokines, interleukin-1 beta (IL-1β) and interleukin-8 (IL-8) emerge as important ones. OBJECTIVE: The purpose of this study was to analyze the location, distribution and concentration of these cytokines in healthy and inflamed dental pulps. MATERIAL AND METHODS: Twenty pulps, obtained from healthy third molars (n=10) and from pulpectomies (n=10) were used for the study, with half of each group used for immunohistochemistry and half for protein extraction and ELISA assays. Fibroblasts obtained from healthy dental pulps, stimulated or not by Escherichia coli lipopolysaccharide (LPS), in order to simulate aggression on the cell cultures, were also used and analyzed by ELISA for IL-1β and IL-8 as complementary information. Data obtained from immunohistochemistry were qualitatively analyzed. Data obtained from ELISA assays (tissue and cells) were statistically treated by the t-test (p
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spelling Interleukin-1 beta and interleukin-8 in healthy and inflamed dental pulps Dental pulpInflammationCytokines After aggression to the dental pulp, some cells produce cytokines in order to start and control the inflammatory process. Among these cytokines, interleukin-1 beta (IL-1β) and interleukin-8 (IL-8) emerge as important ones. OBJECTIVE: The purpose of this study was to analyze the location, distribution and concentration of these cytokines in healthy and inflamed dental pulps. MATERIAL AND METHODS: Twenty pulps, obtained from healthy third molars (n=10) and from pulpectomies (n=10) were used for the study, with half of each group used for immunohistochemistry and half for protein extraction and ELISA assays. Fibroblasts obtained from healthy dental pulps, stimulated or not by Escherichia coli lipopolysaccharide (LPS), in order to simulate aggression on the cell cultures, were also used and analyzed by ELISA for IL-1β and IL-8 as complementary information. Data obtained from immunohistochemistry were qualitatively analyzed. Data obtained from ELISA assays (tissue and cells) were statistically treated by the t-test (pUniversidade de São Paulo. Faculdade de Odontologia de Bauru2009-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/373110.1590/S1678-77572009000500031Journal of Applied Oral Science; Vol. 17 No. 5 (2009); 527-532 Journal of Applied Oral Science; Vol. 17 Núm. 5 (2009); 527-532 Journal of Applied Oral Science; v. 17 n. 5 (2009); 527-532 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3731/4421Copyright (c) 2009 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessSilva, Alessandra Cecília OliveiraFaria, Miriam RubioFontes, AlexandraCampos, Marcia SampaioCavalcanti, Bruno Neves2012-04-27T12:06:57Zoai:revistas.usp.br:article/3731Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T12:06:57Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Interleukin-1 beta and interleukin-8 in healthy and inflamed dental pulps
title Interleukin-1 beta and interleukin-8 in healthy and inflamed dental pulps
spellingShingle Interleukin-1 beta and interleukin-8 in healthy and inflamed dental pulps
Silva, Alessandra Cecília Oliveira
Dental pulp
Inflammation
Cytokines
title_short Interleukin-1 beta and interleukin-8 in healthy and inflamed dental pulps
title_full Interleukin-1 beta and interleukin-8 in healthy and inflamed dental pulps
title_fullStr Interleukin-1 beta and interleukin-8 in healthy and inflamed dental pulps
title_full_unstemmed Interleukin-1 beta and interleukin-8 in healthy and inflamed dental pulps
title_sort Interleukin-1 beta and interleukin-8 in healthy and inflamed dental pulps
author Silva, Alessandra Cecília Oliveira
author_facet Silva, Alessandra Cecília Oliveira
Faria, Miriam Rubio
Fontes, Alexandra
Campos, Marcia Sampaio
Cavalcanti, Bruno Neves
author_role author
author2 Faria, Miriam Rubio
Fontes, Alexandra
Campos, Marcia Sampaio
Cavalcanti, Bruno Neves
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Silva, Alessandra Cecília Oliveira
Faria, Miriam Rubio
Fontes, Alexandra
Campos, Marcia Sampaio
Cavalcanti, Bruno Neves
dc.subject.por.fl_str_mv Dental pulp
Inflammation
Cytokines
topic Dental pulp
Inflammation
Cytokines
description After aggression to the dental pulp, some cells produce cytokines in order to start and control the inflammatory process. Among these cytokines, interleukin-1 beta (IL-1β) and interleukin-8 (IL-8) emerge as important ones. OBJECTIVE: The purpose of this study was to analyze the location, distribution and concentration of these cytokines in healthy and inflamed dental pulps. MATERIAL AND METHODS: Twenty pulps, obtained from healthy third molars (n=10) and from pulpectomies (n=10) were used for the study, with half of each group used for immunohistochemistry and half for protein extraction and ELISA assays. Fibroblasts obtained from healthy dental pulps, stimulated or not by Escherichia coli lipopolysaccharide (LPS), in order to simulate aggression on the cell cultures, were also used and analyzed by ELISA for IL-1β and IL-8 as complementary information. Data obtained from immunohistochemistry were qualitatively analyzed. Data obtained from ELISA assays (tissue and cells) were statistically treated by the t-test (p
publishDate 2009
dc.date.none.fl_str_mv 2009-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/3731
10.1590/S1678-77572009000500031
url https://www.revistas.usp.br/jaos/article/view/3731
identifier_str_mv 10.1590/S1678-77572009000500031
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/3731/4421
dc.rights.driver.fl_str_mv Copyright (c) 2009 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2009 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 17 No. 5 (2009); 527-532
Journal of Applied Oral Science; Vol. 17 Núm. 5 (2009); 527-532
Journal of Applied Oral Science; v. 17 n. 5 (2009); 527-532
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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