Topical application of bFGF on acid-conditioned and non-conditioned dentin: effect on cell proliferation and gene expression in cells relevant for periodontal regeneration

Detalhes bibliográficos
Autor(a) principal: Rocha, Fernanda Regina Godoy
Data de Publicação: 2017
Outros Autores: Souza, João Antônio Chaves de, Guimarães-Stabili, Morgana Rodrigues, Sampaio, José Eduardo Cezar, Rossa Junior, Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/142429
Resumo: Periodontal regeneration is still a challenge in terms of predictability and magnitude of effect. In this study we assess the biological effects of combining chemical root conditioning and biological mediators on three relevant cell types for periodontal regeneration. Material and Methods: Bovine dentin slices were conditioned with 25% citric acid followed by topical application of basic fibroblast growth factor (bFGF, 10 and 50 ng). We used ELISA to assess the dynamics of bFGF release from the dentin surface and RT-qPCR to study the expression of Runx2, Col1a1, Bglap and fibronectin by periodontal ligament (PDL) fibroblasts, cementoblasts and bone marrow stromal cells (BMSC) grown onto these dentin slices. We also assessed the effects of topical application of bFGF on cell proliferation by quantification of genomic DNA. Results: Acid conditioning significantly increased the release of bFGF from dentin slices. Overall, bFGF application significantly (p
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spelling Topical application of bFGF on acid-conditioned and non-conditioned dentin: effect on cell proliferation and gene expression in cells relevant for periodontal regenerationIntercellular signaling peptides and proteinsPeriodontal ligamentDental cementumBone marrowCells Periodontal regeneration is still a challenge in terms of predictability and magnitude of effect. In this study we assess the biological effects of combining chemical root conditioning and biological mediators on three relevant cell types for periodontal regeneration. Material and Methods: Bovine dentin slices were conditioned with 25% citric acid followed by topical application of basic fibroblast growth factor (bFGF, 10 and 50 ng). We used ELISA to assess the dynamics of bFGF release from the dentin surface and RT-qPCR to study the expression of Runx2, Col1a1, Bglap and fibronectin by periodontal ligament (PDL) fibroblasts, cementoblasts and bone marrow stromal cells (BMSC) grown onto these dentin slices. We also assessed the effects of topical application of bFGF on cell proliferation by quantification of genomic DNA. Results: Acid conditioning significantly increased the release of bFGF from dentin slices. Overall, bFGF application significantly (pUniversidade de São Paulo. Faculdade de Odontologia de Bauru2017-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/14242910.1590/1678-7757-2017-0051Journal of Applied Oral Science; Vol. 25 No. 6 (2017); 689-699Journal of Applied Oral Science; Vol. 25 Núm. 6 (2017); 689-699Journal of Applied Oral Science; v. 25 n. 6 (2017); 689-6991678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/142429/137538Copyright (c) 2017 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessRocha, Fernanda Regina GodoySouza, João Antônio Chaves deGuimarães-Stabili, Morgana RodriguesSampaio, José Eduardo CezarRossa Junior, Carlos2018-01-18T16:07:28Zoai:revistas.usp.br:article/142429Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2018-01-18T16:07:28Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Topical application of bFGF on acid-conditioned and non-conditioned dentin: effect on cell proliferation and gene expression in cells relevant for periodontal regeneration
title Topical application of bFGF on acid-conditioned and non-conditioned dentin: effect on cell proliferation and gene expression in cells relevant for periodontal regeneration
spellingShingle Topical application of bFGF on acid-conditioned and non-conditioned dentin: effect on cell proliferation and gene expression in cells relevant for periodontal regeneration
Rocha, Fernanda Regina Godoy
Intercellular signaling peptides and proteins
Periodontal ligament
Dental cementum
Bone marrow
Cells
title_short Topical application of bFGF on acid-conditioned and non-conditioned dentin: effect on cell proliferation and gene expression in cells relevant for periodontal regeneration
title_full Topical application of bFGF on acid-conditioned and non-conditioned dentin: effect on cell proliferation and gene expression in cells relevant for periodontal regeneration
title_fullStr Topical application of bFGF on acid-conditioned and non-conditioned dentin: effect on cell proliferation and gene expression in cells relevant for periodontal regeneration
title_full_unstemmed Topical application of bFGF on acid-conditioned and non-conditioned dentin: effect on cell proliferation and gene expression in cells relevant for periodontal regeneration
title_sort Topical application of bFGF on acid-conditioned and non-conditioned dentin: effect on cell proliferation and gene expression in cells relevant for periodontal regeneration
author Rocha, Fernanda Regina Godoy
author_facet Rocha, Fernanda Regina Godoy
Souza, João Antônio Chaves de
Guimarães-Stabili, Morgana Rodrigues
Sampaio, José Eduardo Cezar
Rossa Junior, Carlos
author_role author
author2 Souza, João Antônio Chaves de
Guimarães-Stabili, Morgana Rodrigues
Sampaio, José Eduardo Cezar
Rossa Junior, Carlos
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rocha, Fernanda Regina Godoy
Souza, João Antônio Chaves de
Guimarães-Stabili, Morgana Rodrigues
Sampaio, José Eduardo Cezar
Rossa Junior, Carlos
dc.subject.por.fl_str_mv Intercellular signaling peptides and proteins
Periodontal ligament
Dental cementum
Bone marrow
Cells
topic Intercellular signaling peptides and proteins
Periodontal ligament
Dental cementum
Bone marrow
Cells
description Periodontal regeneration is still a challenge in terms of predictability and magnitude of effect. In this study we assess the biological effects of combining chemical root conditioning and biological mediators on three relevant cell types for periodontal regeneration. Material and Methods: Bovine dentin slices were conditioned with 25% citric acid followed by topical application of basic fibroblast growth factor (bFGF, 10 and 50 ng). We used ELISA to assess the dynamics of bFGF release from the dentin surface and RT-qPCR to study the expression of Runx2, Col1a1, Bglap and fibronectin by periodontal ligament (PDL) fibroblasts, cementoblasts and bone marrow stromal cells (BMSC) grown onto these dentin slices. We also assessed the effects of topical application of bFGF on cell proliferation by quantification of genomic DNA. Results: Acid conditioning significantly increased the release of bFGF from dentin slices. Overall, bFGF application significantly (p
publishDate 2017
dc.date.none.fl_str_mv 2017-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/142429
10.1590/1678-7757-2017-0051
url https://www.revistas.usp.br/jaos/article/view/142429
identifier_str_mv 10.1590/1678-7757-2017-0051
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/142429/137538
dc.rights.driver.fl_str_mv Copyright (c) 2017 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 25 No. 6 (2017); 689-699
Journal of Applied Oral Science; Vol. 25 Núm. 6 (2017); 689-699
Journal of Applied Oral Science; v. 25 n. 6 (2017); 689-699
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
_version_ 1800221680466919424

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