Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/3720 |
Resumo: | OBJECTIVE: The aim of this investigation was to evaluate the cytotoxicity of two brands of root canal sealers, epoxy-resin based and zinc oxide-eugenol based, and one commercial calcium hydroxide paste on a monocyte cell line THP-1. MATERIAL AND METHODS: Undiluted (crude extract) and diluted extracts to 10%, 1%, 0.1%, 0.01%, 0.001% and 0.0001% of the sealers were tested for cytotoxicity to THP-1 cells using the trypan blue assay. Extracts were obtained according to ISO standard. Data were analyzed statistically by the Kruskal-Wallis and Mann-Whitney tests at 5% significance level. RESULTS: Crude extract of AH Plus and Fill Canal killed approximately 90% of THP-1 cells versus 36% of THP-1 cells killed by L&C crude extract (p |
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oai:revistas.usp.br:article/3720 |
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USP-17 |
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Journal of applied oral science (Online) |
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Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay AH PlusFill CanalL&CCytotoxicityTrypan blue OBJECTIVE: The aim of this investigation was to evaluate the cytotoxicity of two brands of root canal sealers, epoxy-resin based and zinc oxide-eugenol based, and one commercial calcium hydroxide paste on a monocyte cell line THP-1. MATERIAL AND METHODS: Undiluted (crude extract) and diluted extracts to 10%, 1%, 0.1%, 0.01%, 0.001% and 0.0001% of the sealers were tested for cytotoxicity to THP-1 cells using the trypan blue assay. Extracts were obtained according to ISO standard. Data were analyzed statistically by the Kruskal-Wallis and Mann-Whitney tests at 5% significance level. RESULTS: Crude extract of AH Plus and Fill Canal killed approximately 90% of THP-1 cells versus 36% of THP-1 cells killed by L&C crude extract (pUniversidade de São Paulo. Faculdade de Odontologia de Bauru2009-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/372010.1590/S1678-77572009000500020Journal of Applied Oral Science; Vol. 17 No. 5 (2009); 457-461 Journal of Applied Oral Science; Vol. 17 Núm. 5 (2009); 457-461 Journal of Applied Oral Science; v. 17 n. 5 (2009); 457-461 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3720/4410Copyright (c) 2009 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessCorrea, Gabriela Tavares BeknerVeranio, Gabriel Alves CostaSilva, Licínio EsmaeraldoHirata Junior, RaphaelCoil, Jeffry M.Scelza, Miriam F. Zaccaro2012-04-27T12:06:32Zoai:revistas.usp.br:article/3720Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T12:06:32Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay |
title |
Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay |
spellingShingle |
Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay Correa, Gabriela Tavares Bekner AH Plus Fill Canal L&C Cytotoxicity Trypan blue |
title_short |
Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay |
title_full |
Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay |
title_fullStr |
Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay |
title_full_unstemmed |
Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay |
title_sort |
Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay |
author |
Correa, Gabriela Tavares Bekner |
author_facet |
Correa, Gabriela Tavares Bekner Veranio, Gabriel Alves Costa Silva, Licínio Esmaeraldo Hirata Junior, Raphael Coil, Jeffry M. Scelza, Miriam F. Zaccaro |
author_role |
author |
author2 |
Veranio, Gabriel Alves Costa Silva, Licínio Esmaeraldo Hirata Junior, Raphael Coil, Jeffry M. Scelza, Miriam F. Zaccaro |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Correa, Gabriela Tavares Bekner Veranio, Gabriel Alves Costa Silva, Licínio Esmaeraldo Hirata Junior, Raphael Coil, Jeffry M. Scelza, Miriam F. Zaccaro |
dc.subject.por.fl_str_mv |
AH Plus Fill Canal L&C Cytotoxicity Trypan blue |
topic |
AH Plus Fill Canal L&C Cytotoxicity Trypan blue |
description |
OBJECTIVE: The aim of this investigation was to evaluate the cytotoxicity of two brands of root canal sealers, epoxy-resin based and zinc oxide-eugenol based, and one commercial calcium hydroxide paste on a monocyte cell line THP-1. MATERIAL AND METHODS: Undiluted (crude extract) and diluted extracts to 10%, 1%, 0.1%, 0.01%, 0.001% and 0.0001% of the sealers were tested for cytotoxicity to THP-1 cells using the trypan blue assay. Extracts were obtained according to ISO standard. Data were analyzed statistically by the Kruskal-Wallis and Mann-Whitney tests at 5% significance level. RESULTS: Crude extract of AH Plus and Fill Canal killed approximately 90% of THP-1 cells versus 36% of THP-1 cells killed by L&C crude extract (p |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3720 10.1590/S1678-77572009000500020 |
url |
https://www.revistas.usp.br/jaos/article/view/3720 |
identifier_str_mv |
10.1590/S1678-77572009000500020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3720/4410 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2009 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2009 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 17 No. 5 (2009); 457-461 Journal of Applied Oral Science; Vol. 17 Núm. 5 (2009); 457-461 Journal of Applied Oral Science; v. 17 n. 5 (2009); 457-461 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221674808803328 |