A study of the time necessary for calcium hydroxide to eliminate microorganisms in infected canals

Detalhes bibliográficos
Autor(a) principal: Estrela, Carlos
Data de Publicação: 2003
Outros Autores: Estrela, Cyntia Rodrigues de Araújo, Pécora, Jesus Djalma
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/3127
Resumo: The objective of this study was to analyze the time necessary for calcium hydroxide to eliminate microorganisms in infected canals. A total of 168 human anterior teeth were prepared and sterilized. One hundred sixty two teeth were inoculated with suspensions of S. aureus, E. faecalis, P. aeruginosa, B. subtilis, C. albicans. Three teeth were used as negative control and three as positive control. Root canals were irrigated with saline and filled with calcium hydroxide paste (CHP). At intervals of 1 minute, and at 7, 15, 21, 27, 30, 45, 60, and 90 days, CHP was removed, samples were collected and immersed in Letheen Broth (LB). Microbial growth was analyzed by two methods, turbidity of the culture medium and subculture on a Brain heart Infusion. After looking for medium change, an inoculum of 0.1 mL obtained from LB was transferred to 7 mL of Brain Heart Infusion (BHI), and subsequently incubated at 37°C for 48 hours. Microbial growth was checked by turbidity of the culture medium and in some cases by Gram stain. All assays were carried out in triplicate under aseptic technique. The results indicated that the antimicrobial effect on the cultures of S. aureus, E. faecalis, P. aeruginosa, B. subtilis, C. albicans and one mixed culture in infected canals by CHP occurred in 60 days.
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spelling A study of the time necessary for calcium hydroxide to eliminate microorganisms in infected canals Estudo do tempo necessário para o hidróxido de cálcio eliminar microrganismos em canais infectados Hidróxido de cálcioCavidade da polpa dentáriamicrobiologiaCalcium hydroxideDental pulp cavitymicrobiology The objective of this study was to analyze the time necessary for calcium hydroxide to eliminate microorganisms in infected canals. A total of 168 human anterior teeth were prepared and sterilized. One hundred sixty two teeth were inoculated with suspensions of S. aureus, E. faecalis, P. aeruginosa, B. subtilis, C. albicans. Three teeth were used as negative control and three as positive control. Root canals were irrigated with saline and filled with calcium hydroxide paste (CHP). At intervals of 1 minute, and at 7, 15, 21, 27, 30, 45, 60, and 90 days, CHP was removed, samples were collected and immersed in Letheen Broth (LB). Microbial growth was analyzed by two methods, turbidity of the culture medium and subculture on a Brain heart Infusion. After looking for medium change, an inoculum of 0.1 mL obtained from LB was transferred to 7 mL of Brain Heart Infusion (BHI), and subsequently incubated at 37°C for 48 hours. Microbial growth was checked by turbidity of the culture medium and in some cases by Gram stain. All assays were carried out in triplicate under aseptic technique. The results indicated that the antimicrobial effect on the cultures of S. aureus, E. faecalis, P. aeruginosa, B. subtilis, C. albicans and one mixed culture in infected canals by CHP occurred in 60 days. O objetivo deste estudo foi analisar o tempo necessário para que o hidróxido de cálcio elimine microrganismos em canais infectados. Um total de 168 dentes humanos anteriores foram preparados e esterilizados. Cento e sessenta e dois dentes foram inoculados com suspensões de S. aureus, E. faecalis, P. aeruginosa, B. subtilis e C. albicans. Três dentes foram usados como controle negativo e três como controle positivo. Os canais radiculares foram irrigados com solução fisiológica e preenchidos com pasta de hidróxido de cálcio. Em intervalos de 1 minuto, 7, 15, 21, 27, 30, 45, 60 e 90 dias, a pasta foi removida, amostras foram coletadas e imersas em Letheen Broth. O crescimento microbiano foi analisado por dois métodos, turvação do meio de cultura e subcultura em caldo Brain Heart Infusion (BHI). Após alterações do Letheen Broth, um inóculo de 0,1 mL do meio foi transferido para 7 mL de BHI, e subseqüentemente incubado a 37°C for 48h. O crescimento microbiano foi analisado pela turvação do meio de cultura e em alguns casos pela coloração de Gram. Todos os ensaios foram feitos em triplicata, obedecendo a técnica asséptica. Os resultados indicaram que o efeito antimicrobiano, em culturas de S. aureus, E. faecalis, P. aeruginosa, B. subtilis, C. albicans e uma cultura mixta em canais infectados, ocorreu em 60 dias. Universidade de São Paulo. Faculdade de Odontologia de Bauru2003-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/312710.1590/S1678-77572003000200009Journal of Applied Oral Science; Vol. 11 No. 2 (2003); 133-137 Journal of Applied Oral Science; Vol. 11 Núm. 2 (2003); 133-137 Journal of Applied Oral Science; v. 11 n. 2 (2003); 133-137 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3127/3816Copyright (c) 2003 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessEstrela, CarlosEstrela, Cyntia Rodrigues de AraújoPécora, Jesus Djalma2012-04-27T11:44:12Zoai:revistas.usp.br:article/3127Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T11:44:12Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv A study of the time necessary for calcium hydroxide to eliminate microorganisms in infected canals
Estudo do tempo necessário para o hidróxido de cálcio eliminar microrganismos em canais infectados
title A study of the time necessary for calcium hydroxide to eliminate microorganisms in infected canals
spellingShingle A study of the time necessary for calcium hydroxide to eliminate microorganisms in infected canals
Estrela, Carlos
Hidróxido de cálcio
Cavidade da polpa dentária
microbiologia
Calcium hydroxide
Dental pulp cavity
microbiology
title_short A study of the time necessary for calcium hydroxide to eliminate microorganisms in infected canals
title_full A study of the time necessary for calcium hydroxide to eliminate microorganisms in infected canals
title_fullStr A study of the time necessary for calcium hydroxide to eliminate microorganisms in infected canals
title_full_unstemmed A study of the time necessary for calcium hydroxide to eliminate microorganisms in infected canals
title_sort A study of the time necessary for calcium hydroxide to eliminate microorganisms in infected canals
author Estrela, Carlos
author_facet Estrela, Carlos
Estrela, Cyntia Rodrigues de Araújo
Pécora, Jesus Djalma
author_role author
author2 Estrela, Cyntia Rodrigues de Araújo
Pécora, Jesus Djalma
author2_role author
author
dc.contributor.author.fl_str_mv Estrela, Carlos
Estrela, Cyntia Rodrigues de Araújo
Pécora, Jesus Djalma
dc.subject.por.fl_str_mv Hidróxido de cálcio
Cavidade da polpa dentária
microbiologia
Calcium hydroxide
Dental pulp cavity
microbiology
topic Hidróxido de cálcio
Cavidade da polpa dentária
microbiologia
Calcium hydroxide
Dental pulp cavity
microbiology
description The objective of this study was to analyze the time necessary for calcium hydroxide to eliminate microorganisms in infected canals. A total of 168 human anterior teeth were prepared and sterilized. One hundred sixty two teeth were inoculated with suspensions of S. aureus, E. faecalis, P. aeruginosa, B. subtilis, C. albicans. Three teeth were used as negative control and three as positive control. Root canals were irrigated with saline and filled with calcium hydroxide paste (CHP). At intervals of 1 minute, and at 7, 15, 21, 27, 30, 45, 60, and 90 days, CHP was removed, samples were collected and immersed in Letheen Broth (LB). Microbial growth was analyzed by two methods, turbidity of the culture medium and subculture on a Brain heart Infusion. After looking for medium change, an inoculum of 0.1 mL obtained from LB was transferred to 7 mL of Brain Heart Infusion (BHI), and subsequently incubated at 37°C for 48 hours. Microbial growth was checked by turbidity of the culture medium and in some cases by Gram stain. All assays were carried out in triplicate under aseptic technique. The results indicated that the antimicrobial effect on the cultures of S. aureus, E. faecalis, P. aeruginosa, B. subtilis, C. albicans and one mixed culture in infected canals by CHP occurred in 60 days.
publishDate 2003
dc.date.none.fl_str_mv 2003-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/3127
10.1590/S1678-77572003000200009
url https://www.revistas.usp.br/jaos/article/view/3127
identifier_str_mv 10.1590/S1678-77572003000200009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/3127/3816
dc.rights.driver.fl_str_mv Copyright (c) 2003 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2003 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 11 No. 2 (2003); 133-137
Journal of Applied Oral Science; Vol. 11 Núm. 2 (2003); 133-137
Journal of Applied Oral Science; v. 11 n. 2 (2003); 133-137
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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