Effect of betamethasone and diclofenac sodium on serum and tissue concentration of amoxicillin: in vivo study in rats

Detalhes bibliográficos
Autor(a) principal: Mattos Filho,Thales Rocha
Data de Publicação: 2006
Outros Autores: Junqueira,Marcelo de Souza, Groppo,Francisco Carlos, Motta,Rogério Heládio Lopes, Perazzo,Fábio Ferreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572006000500004
Resumo: OBJECTIVE: Antimicrobial agents in combination with anti-inflammatory drugs have been usually prescribed in both Medicine and Dentistry. However, few scientific reports support this clinical practice. The aim of this study was to evaluate the effect of betamethasone and diclofenac sodium on serum and tissue concentration of amoxicillin in rats. METHODS: Four polyurethane sponges were implanted in the back skin of 48 rats. After seven days the animals were divided into 6 groups (n=8). Group 1: amoxicillin (25 mg/kg); G2: diclofenac sodium (2.5 mg/kg); G3: betamethasone (0.1 mg/kg); G4: diclofenac sodium and amoxicillin; G5: betamethasone and amoxicillin; and G6: 0.9% sodium chloride solution (1.0 mL - control group). All drugs were administered in a single dose. After 90 minutes, the granulomatous tissues of each animal were surgically removed and weighed. Blood was collected from cervical plexus, centrifuged and 10µL of serum was placed on paper discs. In order to estimate amoxicillin concentration, serum and granulomatous tissues were separately submitted to microbiological assay, which used 10(8)cfu/mL of Staphylococcus aureus ATCC 25923 (penicillin-susceptible strain). After incubation (18 hours, 37ºC) the inhibition zones were measured and compared to a regression curve. RESULTS: No inhibition zones were observed for groups 2, 3 and 6. Tissue and serum concentrations of both G1 (4.14µg/g and 2.06µg/mL, respectively) and G5 (3.87µg/g and 1.70µg/mL, respectively) showed statistically significant differences (Kruskal-Wallis, p<0.05) in comparison to G4 (1.45µg/g and 0.41µg/mL, respectively). G1 and G5 did not differ significantly (p>0.05). CONCLUSION: Considering single doses, betamethasone did not interfere with amoxicillin levels but diclofenac sodium reduced both tissue and serum levels of amoxicillin in rats.
id USP-17_8e7a6c5da42291baafff18923cde049b
oai_identifier_str oai:scielo:S1678-77572006000500004
network_acronym_str USP-17
network_name_str Journal of applied oral science (Online)
repository_id_str
spelling Effect of betamethasone and diclofenac sodium on serum and tissue concentration of amoxicillin: in vivo study in ratsAmoxicillinBetamethasoneDrug interactionOBJECTIVE: Antimicrobial agents in combination with anti-inflammatory drugs have been usually prescribed in both Medicine and Dentistry. However, few scientific reports support this clinical practice. The aim of this study was to evaluate the effect of betamethasone and diclofenac sodium on serum and tissue concentration of amoxicillin in rats. METHODS: Four polyurethane sponges were implanted in the back skin of 48 rats. After seven days the animals were divided into 6 groups (n=8). Group 1: amoxicillin (25 mg/kg); G2: diclofenac sodium (2.5 mg/kg); G3: betamethasone (0.1 mg/kg); G4: diclofenac sodium and amoxicillin; G5: betamethasone and amoxicillin; and G6: 0.9% sodium chloride solution (1.0 mL - control group). All drugs were administered in a single dose. After 90 minutes, the granulomatous tissues of each animal were surgically removed and weighed. Blood was collected from cervical plexus, centrifuged and 10µL of serum was placed on paper discs. In order to estimate amoxicillin concentration, serum and granulomatous tissues were separately submitted to microbiological assay, which used 10(8)cfu/mL of Staphylococcus aureus ATCC 25923 (penicillin-susceptible strain). After incubation (18 hours, 37ºC) the inhibition zones were measured and compared to a regression curve. RESULTS: No inhibition zones were observed for groups 2, 3 and 6. Tissue and serum concentrations of both G1 (4.14µg/g and 2.06µg/mL, respectively) and G5 (3.87µg/g and 1.70µg/mL, respectively) showed statistically significant differences (Kruskal-Wallis, p<0.05) in comparison to G4 (1.45µg/g and 0.41µg/mL, respectively). G1 and G5 did not differ significantly (p>0.05). CONCLUSION: Considering single doses, betamethasone did not interfere with amoxicillin levels but diclofenac sodium reduced both tissue and serum levels of amoxicillin in rats.Faculdade De Odontologia De Bauru - USP2006-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572006000500004Journal of Applied Oral Science v.14 n.5 2006reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S1678-77572006000500004info:eu-repo/semantics/openAccessMattos Filho,Thales RochaJunqueira,Marcelo de SouzaGroppo,Francisco CarlosMotta,Rogério Heládio LopesPerazzo,Fábio Ferreiraeng2007-01-10T00:00:00Zoai:scielo:S1678-77572006000500004Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2007-01-10T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Effect of betamethasone and diclofenac sodium on serum and tissue concentration of amoxicillin: in vivo study in rats
title Effect of betamethasone and diclofenac sodium on serum and tissue concentration of amoxicillin: in vivo study in rats
spellingShingle Effect of betamethasone and diclofenac sodium on serum and tissue concentration of amoxicillin: in vivo study in rats
Mattos Filho,Thales Rocha
Amoxicillin
Betamethasone
Drug interaction
title_short Effect of betamethasone and diclofenac sodium on serum and tissue concentration of amoxicillin: in vivo study in rats
title_full Effect of betamethasone and diclofenac sodium on serum and tissue concentration of amoxicillin: in vivo study in rats
title_fullStr Effect of betamethasone and diclofenac sodium on serum and tissue concentration of amoxicillin: in vivo study in rats
title_full_unstemmed Effect of betamethasone and diclofenac sodium on serum and tissue concentration of amoxicillin: in vivo study in rats
title_sort Effect of betamethasone and diclofenac sodium on serum and tissue concentration of amoxicillin: in vivo study in rats
author Mattos Filho,Thales Rocha
author_facet Mattos Filho,Thales Rocha
Junqueira,Marcelo de Souza
Groppo,Francisco Carlos
Motta,Rogério Heládio Lopes
Perazzo,Fábio Ferreira
author_role author
author2 Junqueira,Marcelo de Souza
Groppo,Francisco Carlos
Motta,Rogério Heládio Lopes
Perazzo,Fábio Ferreira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Mattos Filho,Thales Rocha
Junqueira,Marcelo de Souza
Groppo,Francisco Carlos
Motta,Rogério Heládio Lopes
Perazzo,Fábio Ferreira
dc.subject.por.fl_str_mv Amoxicillin
Betamethasone
Drug interaction
topic Amoxicillin
Betamethasone
Drug interaction
description OBJECTIVE: Antimicrobial agents in combination with anti-inflammatory drugs have been usually prescribed in both Medicine and Dentistry. However, few scientific reports support this clinical practice. The aim of this study was to evaluate the effect of betamethasone and diclofenac sodium on serum and tissue concentration of amoxicillin in rats. METHODS: Four polyurethane sponges were implanted in the back skin of 48 rats. After seven days the animals were divided into 6 groups (n=8). Group 1: amoxicillin (25 mg/kg); G2: diclofenac sodium (2.5 mg/kg); G3: betamethasone (0.1 mg/kg); G4: diclofenac sodium and amoxicillin; G5: betamethasone and amoxicillin; and G6: 0.9% sodium chloride solution (1.0 mL - control group). All drugs were administered in a single dose. After 90 minutes, the granulomatous tissues of each animal were surgically removed and weighed. Blood was collected from cervical plexus, centrifuged and 10µL of serum was placed on paper discs. In order to estimate amoxicillin concentration, serum and granulomatous tissues were separately submitted to microbiological assay, which used 10(8)cfu/mL of Staphylococcus aureus ATCC 25923 (penicillin-susceptible strain). After incubation (18 hours, 37ºC) the inhibition zones were measured and compared to a regression curve. RESULTS: No inhibition zones were observed for groups 2, 3 and 6. Tissue and serum concentrations of both G1 (4.14µg/g and 2.06µg/mL, respectively) and G5 (3.87µg/g and 1.70µg/mL, respectively) showed statistically significant differences (Kruskal-Wallis, p<0.05) in comparison to G4 (1.45µg/g and 0.41µg/mL, respectively). G1 and G5 did not differ significantly (p>0.05). CONCLUSION: Considering single doses, betamethasone did not interfere with amoxicillin levels but diclofenac sodium reduced both tissue and serum levels of amoxicillin in rats.
publishDate 2006
dc.date.none.fl_str_mv 2006-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572006000500004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572006000500004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1678-77572006000500004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
dc.source.none.fl_str_mv Journal of Applied Oral Science v.14 n.5 2006
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
_version_ 1748936434326175744