QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model

Detalhes bibliográficos
Autor(a) principal: GRÜNDLING, Grasiela Longhi
Data de Publicação: 2015
Outros Autores: MELO, Tiago André Fontoura de, MONTAGNER, Francisco, SCARPARO, Roberta Kochenborger, VIER-PELISSER, Fabiana Vieira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/104938
Resumo: AbstractThe presence of endotoxin inside the root canal has been associated with periapical inflammation, bone resorption and symptomatic conditions.Objectives To determine, in vitro, the effect of QMix® and other three root canal irrigants in reducing the endotoxin content in root canals.Material and Methods Root canals of single-rooted teeth were prepared. Samples were detoxified with Co-60 irradiation and inoculated with E. coli LPS (24 h, at 37°C). After that period, samples were divided into 4 groups, according to the irrigation solution tested: QMix®, 17% EDTA, 2% chlorhexidine solution (CHX), and 3% sodium hypochlorite (NaOCl). LPS quantification was determined by Limulus Amebocyte Lysate (LAL) assay. The initial counting of endotoxins for all samples, and the determination of LPS levels in non-contaminated teeth and in contaminated teeth exposed only to non-pyrogenic water, were used as controls.Results QMix® reduced LPS levels, with a median value of 1.11 endotoxins units (EU)/mL (p
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spelling QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model AbstractThe presence of endotoxin inside the root canal has been associated with periapical inflammation, bone resorption and symptomatic conditions.Objectives To determine, in vitro, the effect of QMix® and other three root canal irrigants in reducing the endotoxin content in root canals.Material and Methods Root canals of single-rooted teeth were prepared. Samples were detoxified with Co-60 irradiation and inoculated with E. coli LPS (24 h, at 37°C). After that period, samples were divided into 4 groups, according to the irrigation solution tested: QMix®, 17% EDTA, 2% chlorhexidine solution (CHX), and 3% sodium hypochlorite (NaOCl). LPS quantification was determined by Limulus Amebocyte Lysate (LAL) assay. The initial counting of endotoxins for all samples, and the determination of LPS levels in non-contaminated teeth and in contaminated teeth exposed only to non-pyrogenic water, were used as controls.Results QMix® reduced LPS levels, with a median value of 1.11 endotoxins units (EU)/mL (pUniversidade de São Paulo. Faculdade de Odontologia de Bauru2015-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/10493810.1590/1678-775720140488Journal of Applied Oral Science; Vol. 23 No. 4 (2015); 431-435Journal of Applied Oral Science; Vol. 23 Núm. 4 (2015); 431-435Journal of Applied Oral Science; v. 23 n. 4 (2015); 431-4351678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/104938/103728Copyright (c) 2015 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessGRÜNDLING, Grasiela LonghiMELO, Tiago André Fontoura deMONTAGNER, FranciscoSCARPARO, Roberta KochenborgerVIER-PELISSER, Fabiana Vieira2015-09-25T18:45:40Zoai:revistas.usp.br:article/104938Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2015-09-25T18:45:40Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model
title QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model
spellingShingle QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model
GRÜNDLING, Grasiela Longhi
title_short QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model
title_full QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model
title_fullStr QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model
title_full_unstemmed QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model
title_sort QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model
author GRÜNDLING, Grasiela Longhi
author_facet GRÜNDLING, Grasiela Longhi
MELO, Tiago André Fontoura de
MONTAGNER, Francisco
SCARPARO, Roberta Kochenborger
VIER-PELISSER, Fabiana Vieira
author_role author
author2 MELO, Tiago André Fontoura de
MONTAGNER, Francisco
SCARPARO, Roberta Kochenborger
VIER-PELISSER, Fabiana Vieira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv GRÜNDLING, Grasiela Longhi
MELO, Tiago André Fontoura de
MONTAGNER, Francisco
SCARPARO, Roberta Kochenborger
VIER-PELISSER, Fabiana Vieira
description AbstractThe presence of endotoxin inside the root canal has been associated with periapical inflammation, bone resorption and symptomatic conditions.Objectives To determine, in vitro, the effect of QMix® and other three root canal irrigants in reducing the endotoxin content in root canals.Material and Methods Root canals of single-rooted teeth were prepared. Samples were detoxified with Co-60 irradiation and inoculated with E. coli LPS (24 h, at 37°C). After that period, samples were divided into 4 groups, according to the irrigation solution tested: QMix®, 17% EDTA, 2% chlorhexidine solution (CHX), and 3% sodium hypochlorite (NaOCl). LPS quantification was determined by Limulus Amebocyte Lysate (LAL) assay. The initial counting of endotoxins for all samples, and the determination of LPS levels in non-contaminated teeth and in contaminated teeth exposed only to non-pyrogenic water, were used as controls.Results QMix® reduced LPS levels, with a median value of 1.11 endotoxins units (EU)/mL (p
publishDate 2015
dc.date.none.fl_str_mv 2015-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/104938
10.1590/1678-775720140488
url https://www.revistas.usp.br/jaos/article/view/104938
identifier_str_mv 10.1590/1678-775720140488
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/104938/103728
dc.rights.driver.fl_str_mv Copyright (c) 2015 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2015 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 23 No. 4 (2015); 431-435
Journal of Applied Oral Science; Vol. 23 Núm. 4 (2015); 431-435
Journal of Applied Oral Science; v. 23 n. 4 (2015); 431-435
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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