QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/104938 |
Resumo: | AbstractThe presence of endotoxin inside the root canal has been associated with periapical inflammation, bone resorption and symptomatic conditions.Objectives To determine, in vitro, the effect of QMix® and other three root canal irrigants in reducing the endotoxin content in root canals.Material and Methods Root canals of single-rooted teeth were prepared. Samples were detoxified with Co-60 irradiation and inoculated with E. coli LPS (24 h, at 37°C). After that period, samples were divided into 4 groups, according to the irrigation solution tested: QMix®, 17% EDTA, 2% chlorhexidine solution (CHX), and 3% sodium hypochlorite (NaOCl). LPS quantification was determined by Limulus Amebocyte Lysate (LAL) assay. The initial counting of endotoxins for all samples, and the determination of LPS levels in non-contaminated teeth and in contaminated teeth exposed only to non-pyrogenic water, were used as controls.Results QMix® reduced LPS levels, with a median value of 1.11 endotoxins units (EU)/mL (p |
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oai:revistas.usp.br:article/104938 |
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USP-17 |
network_name_str |
Journal of applied oral science (Online) |
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spelling |
QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model AbstractThe presence of endotoxin inside the root canal has been associated with periapical inflammation, bone resorption and symptomatic conditions.Objectives To determine, in vitro, the effect of QMix® and other three root canal irrigants in reducing the endotoxin content in root canals.Material and Methods Root canals of single-rooted teeth were prepared. Samples were detoxified with Co-60 irradiation and inoculated with E. coli LPS (24 h, at 37°C). After that period, samples were divided into 4 groups, according to the irrigation solution tested: QMix®, 17% EDTA, 2% chlorhexidine solution (CHX), and 3% sodium hypochlorite (NaOCl). LPS quantification was determined by Limulus Amebocyte Lysate (LAL) assay. The initial counting of endotoxins for all samples, and the determination of LPS levels in non-contaminated teeth and in contaminated teeth exposed only to non-pyrogenic water, were used as controls.Results QMix® reduced LPS levels, with a median value of 1.11 endotoxins units (EU)/mL (pUniversidade de São Paulo. Faculdade de Odontologia de Bauru2015-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/10493810.1590/1678-775720140488Journal of Applied Oral Science; Vol. 23 No. 4 (2015); 431-435Journal of Applied Oral Science; Vol. 23 Núm. 4 (2015); 431-435Journal of Applied Oral Science; v. 23 n. 4 (2015); 431-4351678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/104938/103728Copyright (c) 2015 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessGRÜNDLING, Grasiela LonghiMELO, Tiago André Fontoura deMONTAGNER, FranciscoSCARPARO, Roberta KochenborgerVIER-PELISSER, Fabiana Vieira2015-09-25T18:45:40Zoai:revistas.usp.br:article/104938Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2015-09-25T18:45:40Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model |
title |
QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model |
spellingShingle |
QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model GRÜNDLING, Grasiela Longhi |
title_short |
QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model |
title_full |
QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model |
title_fullStr |
QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model |
title_full_unstemmed |
QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model |
title_sort |
QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model |
author |
GRÜNDLING, Grasiela Longhi |
author_facet |
GRÜNDLING, Grasiela Longhi MELO, Tiago André Fontoura de MONTAGNER, Francisco SCARPARO, Roberta Kochenborger VIER-PELISSER, Fabiana Vieira |
author_role |
author |
author2 |
MELO, Tiago André Fontoura de MONTAGNER, Francisco SCARPARO, Roberta Kochenborger VIER-PELISSER, Fabiana Vieira |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
GRÜNDLING, Grasiela Longhi MELO, Tiago André Fontoura de MONTAGNER, Francisco SCARPARO, Roberta Kochenborger VIER-PELISSER, Fabiana Vieira |
description |
AbstractThe presence of endotoxin inside the root canal has been associated with periapical inflammation, bone resorption and symptomatic conditions.Objectives To determine, in vitro, the effect of QMix® and other three root canal irrigants in reducing the endotoxin content in root canals.Material and Methods Root canals of single-rooted teeth were prepared. Samples were detoxified with Co-60 irradiation and inoculated with E. coli LPS (24 h, at 37°C). After that period, samples were divided into 4 groups, according to the irrigation solution tested: QMix®, 17% EDTA, 2% chlorhexidine solution (CHX), and 3% sodium hypochlorite (NaOCl). LPS quantification was determined by Limulus Amebocyte Lysate (LAL) assay. The initial counting of endotoxins for all samples, and the determination of LPS levels in non-contaminated teeth and in contaminated teeth exposed only to non-pyrogenic water, were used as controls.Results QMix® reduced LPS levels, with a median value of 1.11 endotoxins units (EU)/mL (p |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/104938 10.1590/1678-775720140488 |
url |
https://www.revistas.usp.br/jaos/article/view/104938 |
identifier_str_mv |
10.1590/1678-775720140488 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/104938/103728 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 23 No. 4 (2015); 431-435 Journal of Applied Oral Science; Vol. 23 Núm. 4 (2015); 431-435 Journal of Applied Oral Science; v. 23 n. 4 (2015); 431-435 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221678833238016 |