Quantitative analysis of S. mutans and S. sobrinus cultivated independently and adhered to polished orthodontic composite resins
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/47988 |
Resumo: | In Orthodontics, fixed appliances placed in the oral cavity are colonized by microorganisms. OBJECTIVE: The purpose of this study was to quantitatively determine the independent bacterial colonization of S. mutans and S. sobrinus in orthodontic composite resins. MATERIAL AND METHODS: Seven orthodontic composite adhesives for bonding brackets were selected and classified into 14 groups; (GIm, GIs) Enlight, (GIIm, GIIs) Grengloo, (GIIIm, GIIIs) Kurasper F, (GIVm, GIVs) BeautyOrtho Bond, (GVm, GVs) Transbond CC, (GVIm, GVIs) Turbo Bond II, (GVIIm, GVIIs) Blugloo. 60 blocks of 4x4x1 mm of each orthodontic composite resin were made (total 420 blocks), and gently polished with sand-paper and ultrasonically cleaned. S. mutans and S. sobrinus were independently cultivated. For the quantitative analysis, a radioactive marker was used to codify the bacteria (³H) adhered to the surface of the materials. The blocks were submerged in a solution with microorganisms previously radiolabeled and separated (210 blocks for S. mutans and 210 blocks for S. sobrinus) for 2 hours at 37ºC. Next, the blocks were placed in a combustion system, to capture the residues and measure the radiation. The statistical analysis was calculated with the ANOVA test (Sheffè post-hoc). RESULTS: Significant differences of bacterial adhesion were found amongst the groups. In the GIm and GIs the significant lowest scores for both microorganisms were shown; in contrast, the values of GVII for both bacteria were significantly the highest. CONCLUSIONS: This study showed that the orthodontic composite resin evaluated in the GIm and GIs, obtained the lowest adherence of S. mutans and S. sobrinus, which may reduce the enamel demineralization and the risk of white spot lesion formation. |
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Journal of applied oral science (Online) |
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Quantitative analysis of S. mutans and S. sobrinus cultivated independently and adhered to polished orthodontic composite resinsRadioisotopeWhite spotComposite resinsStreptococcus mutansStreptococcus sobrinusIn Orthodontics, fixed appliances placed in the oral cavity are colonized by microorganisms. OBJECTIVE: The purpose of this study was to quantitatively determine the independent bacterial colonization of S. mutans and S. sobrinus in orthodontic composite resins. MATERIAL AND METHODS: Seven orthodontic composite adhesives for bonding brackets were selected and classified into 14 groups; (GIm, GIs) Enlight, (GIIm, GIIs) Grengloo, (GIIIm, GIIIs) Kurasper F, (GIVm, GIVs) BeautyOrtho Bond, (GVm, GVs) Transbond CC, (GVIm, GVIs) Turbo Bond II, (GVIIm, GVIIs) Blugloo. 60 blocks of 4x4x1 mm of each orthodontic composite resin were made (total 420 blocks), and gently polished with sand-paper and ultrasonically cleaned. S. mutans and S. sobrinus were independently cultivated. For the quantitative analysis, a radioactive marker was used to codify the bacteria (³H) adhered to the surface of the materials. The blocks were submerged in a solution with microorganisms previously radiolabeled and separated (210 blocks for S. mutans and 210 blocks for S. sobrinus) for 2 hours at 37ºC. Next, the blocks were placed in a combustion system, to capture the residues and measure the radiation. The statistical analysis was calculated with the ANOVA test (Sheffè post-hoc). RESULTS: Significant differences of bacterial adhesion were found amongst the groups. In the GIm and GIs the significant lowest scores for both microorganisms were shown; in contrast, the values of GVII for both bacteria were significantly the highest. CONCLUSIONS: This study showed that the orthodontic composite resin evaluated in the GIm and GIs, obtained the lowest adherence of S. mutans and S. sobrinus, which may reduce the enamel demineralization and the risk of white spot lesion formation.Universidade de São Paulo. Faculdade de Odontologia de Bauru2012-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/4798810.1590/S1678-77572012000500009Journal of Applied Oral Science; Vol. 20 No. 5 (2012); 544-549Journal of Applied Oral Science; Vol. 20 Núm. 5 (2012); 544-549Journal of Applied Oral Science; v. 20 n. 5 (2012); 544-5491678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/47988/51728Copyright (c) 2012 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessVelazquez-Enriquez, UlisesScougall-Vilchis, Rogelio JoseContreras-Bulnes, RosaliaFlores-Estrada, JaimeUematsu, ShinsukeYamaguchi, Ryozo2012-12-13T12:10:34Zoai:revistas.usp.br:article/47988Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-12-13T12:10:34Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Quantitative analysis of S. mutans and S. sobrinus cultivated independently and adhered to polished orthodontic composite resins |
title |
Quantitative analysis of S. mutans and S. sobrinus cultivated independently and adhered to polished orthodontic composite resins |
spellingShingle |
Quantitative analysis of S. mutans and S. sobrinus cultivated independently and adhered to polished orthodontic composite resins Velazquez-Enriquez, Ulises Radioisotope White spot Composite resins Streptococcus mutans Streptococcus sobrinus |
title_short |
Quantitative analysis of S. mutans and S. sobrinus cultivated independently and adhered to polished orthodontic composite resins |
title_full |
Quantitative analysis of S. mutans and S. sobrinus cultivated independently and adhered to polished orthodontic composite resins |
title_fullStr |
Quantitative analysis of S. mutans and S. sobrinus cultivated independently and adhered to polished orthodontic composite resins |
title_full_unstemmed |
Quantitative analysis of S. mutans and S. sobrinus cultivated independently and adhered to polished orthodontic composite resins |
title_sort |
Quantitative analysis of S. mutans and S. sobrinus cultivated independently and adhered to polished orthodontic composite resins |
author |
Velazquez-Enriquez, Ulises |
author_facet |
Velazquez-Enriquez, Ulises Scougall-Vilchis, Rogelio Jose Contreras-Bulnes, Rosalia Flores-Estrada, Jaime Uematsu, Shinsuke Yamaguchi, Ryozo |
author_role |
author |
author2 |
Scougall-Vilchis, Rogelio Jose Contreras-Bulnes, Rosalia Flores-Estrada, Jaime Uematsu, Shinsuke Yamaguchi, Ryozo |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Velazquez-Enriquez, Ulises Scougall-Vilchis, Rogelio Jose Contreras-Bulnes, Rosalia Flores-Estrada, Jaime Uematsu, Shinsuke Yamaguchi, Ryozo |
dc.subject.por.fl_str_mv |
Radioisotope White spot Composite resins Streptococcus mutans Streptococcus sobrinus |
topic |
Radioisotope White spot Composite resins Streptococcus mutans Streptococcus sobrinus |
description |
In Orthodontics, fixed appliances placed in the oral cavity are colonized by microorganisms. OBJECTIVE: The purpose of this study was to quantitatively determine the independent bacterial colonization of S. mutans and S. sobrinus in orthodontic composite resins. MATERIAL AND METHODS: Seven orthodontic composite adhesives for bonding brackets were selected and classified into 14 groups; (GIm, GIs) Enlight, (GIIm, GIIs) Grengloo, (GIIIm, GIIIs) Kurasper F, (GIVm, GIVs) BeautyOrtho Bond, (GVm, GVs) Transbond CC, (GVIm, GVIs) Turbo Bond II, (GVIIm, GVIIs) Blugloo. 60 blocks of 4x4x1 mm of each orthodontic composite resin were made (total 420 blocks), and gently polished with sand-paper and ultrasonically cleaned. S. mutans and S. sobrinus were independently cultivated. For the quantitative analysis, a radioactive marker was used to codify the bacteria (³H) adhered to the surface of the materials. The blocks were submerged in a solution with microorganisms previously radiolabeled and separated (210 blocks for S. mutans and 210 blocks for S. sobrinus) for 2 hours at 37ºC. Next, the blocks were placed in a combustion system, to capture the residues and measure the radiation. The statistical analysis was calculated with the ANOVA test (Sheffè post-hoc). RESULTS: Significant differences of bacterial adhesion were found amongst the groups. In the GIm and GIs the significant lowest scores for both microorganisms were shown; in contrast, the values of GVII for both bacteria were significantly the highest. CONCLUSIONS: This study showed that the orthodontic composite resin evaluated in the GIm and GIs, obtained the lowest adherence of S. mutans and S. sobrinus, which may reduce the enamel demineralization and the risk of white spot lesion formation. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/47988 10.1590/S1678-77572012000500009 |
url |
https://www.revistas.usp.br/jaos/article/view/47988 |
identifier_str_mv |
10.1590/S1678-77572012000500009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/47988/51728 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2012 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2012 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 20 No. 5 (2012); 544-549 Journal of Applied Oral Science; Vol. 20 Núm. 5 (2012); 544-549 Journal of Applied Oral Science; v. 20 n. 5 (2012); 544-549 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221677488963584 |