Evaluation of the remineralizing capacity of silver diamine fluoride on demineralized dentin under pH-cycling conditions
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/210005 |
Resumo: | Objective: (1) to determine the effects of the silver diamine fluoride (SDF) and sodium fluoride (NaF) in demineralized dentin exposed to an acid challenge by pH-cycling, (2) to evaluate the remineralizing capacity of SDF/NaF products based on the physicochemical and mechanical properties of the treated dentin surfaces. Methodology: In total, 57 human molars were evaluated in different stages of the experimental period: sound dentin – negative control (Stage 1), demineralized dentin – positive control (Stage 2), and dentin treated with SDF/NaF products + pH-c (Stage 3). Several commercial products were used for the SDF treatment: Saforide, RivaStar, and Cariestop. The mineral composition and crystalline and morphological characteristics of the dentin samples from each experimental stage were evaluated by infrared spectroscopy (ATR-FTIR), X-ray diffraction, and electron microscopy (SEM-EDX) analytical techniques. Moreover, the mechanical response of the samples was analyzed by means of the three-point bending test. Statistics were estimated for ATR-FTIR variables by Wilcoxon test, while the mechanical data analyses were performed using Kruskal-Wallis and Mann Whitney U tests. Results: Regarding the chemical composition, we observed a higher mineral/organic content in the SDF/NaF treated dentin + pH-c groups (Stage 3) than in the positive control groups (Saforide p=0.03; Cariestop p=0.008; RivaStar p=0.013; NaF p=0.04). The XRD results showed that the crystallite size of hydroxyapatite increased in the SDF/NaF treated dentin + pH-c groups (between +63% in RivaStar to +108% in Saforide), regarding the positive control. SEM images showed that after application of the SDF/NaF products a crystalline precipitate formed on the dentin surface and partially filled the dentin tubules. The flexural strength (MPa) values were higher in the dentin treated with SDF/NaF + pH-c (Stage 3) compared to the positive control groups (Saforide p=0.002; Cariestop p=0.04; RivaStar p=0.04; NaF p=0.02). Conclusions: The application of SDF/NaF affected the physicochemical and mechanical properties of demineralized dentin. According to the results, the use of SFD/NaF had a remineralizing effect on the dentin surface even under acid challenge. |
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Journal of applied oral science (Online) |
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Evaluation of the remineralizing capacity of silver diamine fluoride on demineralized dentin under pH-cycling conditionsDentinSodium fluorideBiomineralizationDental cariesSilver compoundsSilver diamineCrystallizationObjective: (1) to determine the effects of the silver diamine fluoride (SDF) and sodium fluoride (NaF) in demineralized dentin exposed to an acid challenge by pH-cycling, (2) to evaluate the remineralizing capacity of SDF/NaF products based on the physicochemical and mechanical properties of the treated dentin surfaces. Methodology: In total, 57 human molars were evaluated in different stages of the experimental period: sound dentin – negative control (Stage 1), demineralized dentin – positive control (Stage 2), and dentin treated with SDF/NaF products + pH-c (Stage 3). Several commercial products were used for the SDF treatment: Saforide, RivaStar, and Cariestop. The mineral composition and crystalline and morphological characteristics of the dentin samples from each experimental stage were evaluated by infrared spectroscopy (ATR-FTIR), X-ray diffraction, and electron microscopy (SEM-EDX) analytical techniques. Moreover, the mechanical response of the samples was analyzed by means of the three-point bending test. Statistics were estimated for ATR-FTIR variables by Wilcoxon test, while the mechanical data analyses were performed using Kruskal-Wallis and Mann Whitney U tests. Results: Regarding the chemical composition, we observed a higher mineral/organic content in the SDF/NaF treated dentin + pH-c groups (Stage 3) than in the positive control groups (Saforide p=0.03; Cariestop p=0.008; RivaStar p=0.013; NaF p=0.04). The XRD results showed that the crystallite size of hydroxyapatite increased in the SDF/NaF treated dentin + pH-c groups (between +63% in RivaStar to +108% in Saforide), regarding the positive control. SEM images showed that after application of the SDF/NaF products a crystalline precipitate formed on the dentin surface and partially filled the dentin tubules. The flexural strength (MPa) values were higher in the dentin treated with SDF/NaF + pH-c (Stage 3) compared to the positive control groups (Saforide p=0.002; Cariestop p=0.04; RivaStar p=0.04; NaF p=0.02). Conclusions: The application of SDF/NaF affected the physicochemical and mechanical properties of demineralized dentin. According to the results, the use of SFD/NaF had a remineralizing effect on the dentin surface even under acid challenge.Universidade de São Paulo. Faculdade de Odontologia de Bauru2023-03-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/21000510.1590/1678-7757-2022-0306 Journal of Applied Oral Science; Vol. 31 (2023); e20220306Journal of Applied Oral Science; Vol. 31 (2023); e20220306Journal of Applied Oral Science; v. 31 (2023); e202203061678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/210005/192556Copyright (c) 2023 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCifuentes-Jiménez, Carolina CeciliaBolaños-Carmona, María VictoriaEnrich-Essvein, TatianaGonzález-López, SantiagoÁlvarez-Lloret, Pedro2023-03-29T14:35:55Zoai:revistas.usp.br:article/210005Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2023-03-29T14:35:55Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Evaluation of the remineralizing capacity of silver diamine fluoride on demineralized dentin under pH-cycling conditions |
title |
Evaluation of the remineralizing capacity of silver diamine fluoride on demineralized dentin under pH-cycling conditions |
spellingShingle |
Evaluation of the remineralizing capacity of silver diamine fluoride on demineralized dentin under pH-cycling conditions Cifuentes-Jiménez, Carolina Cecilia Dentin Sodium fluoride Biomineralization Dental caries Silver compounds Silver diamine Crystallization |
title_short |
Evaluation of the remineralizing capacity of silver diamine fluoride on demineralized dentin under pH-cycling conditions |
title_full |
Evaluation of the remineralizing capacity of silver diamine fluoride on demineralized dentin under pH-cycling conditions |
title_fullStr |
Evaluation of the remineralizing capacity of silver diamine fluoride on demineralized dentin under pH-cycling conditions |
title_full_unstemmed |
Evaluation of the remineralizing capacity of silver diamine fluoride on demineralized dentin under pH-cycling conditions |
title_sort |
Evaluation of the remineralizing capacity of silver diamine fluoride on demineralized dentin under pH-cycling conditions |
author |
Cifuentes-Jiménez, Carolina Cecilia |
author_facet |
Cifuentes-Jiménez, Carolina Cecilia Bolaños-Carmona, María Victoria Enrich-Essvein, Tatiana González-López, Santiago Álvarez-Lloret, Pedro |
author_role |
author |
author2 |
Bolaños-Carmona, María Victoria Enrich-Essvein, Tatiana González-López, Santiago Álvarez-Lloret, Pedro |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Cifuentes-Jiménez, Carolina Cecilia Bolaños-Carmona, María Victoria Enrich-Essvein, Tatiana González-López, Santiago Álvarez-Lloret, Pedro |
dc.subject.por.fl_str_mv |
Dentin Sodium fluoride Biomineralization Dental caries Silver compounds Silver diamine Crystallization |
topic |
Dentin Sodium fluoride Biomineralization Dental caries Silver compounds Silver diamine Crystallization |
description |
Objective: (1) to determine the effects of the silver diamine fluoride (SDF) and sodium fluoride (NaF) in demineralized dentin exposed to an acid challenge by pH-cycling, (2) to evaluate the remineralizing capacity of SDF/NaF products based on the physicochemical and mechanical properties of the treated dentin surfaces. Methodology: In total, 57 human molars were evaluated in different stages of the experimental period: sound dentin – negative control (Stage 1), demineralized dentin – positive control (Stage 2), and dentin treated with SDF/NaF products + pH-c (Stage 3). Several commercial products were used for the SDF treatment: Saforide, RivaStar, and Cariestop. The mineral composition and crystalline and morphological characteristics of the dentin samples from each experimental stage were evaluated by infrared spectroscopy (ATR-FTIR), X-ray diffraction, and electron microscopy (SEM-EDX) analytical techniques. Moreover, the mechanical response of the samples was analyzed by means of the three-point bending test. Statistics were estimated for ATR-FTIR variables by Wilcoxon test, while the mechanical data analyses were performed using Kruskal-Wallis and Mann Whitney U tests. Results: Regarding the chemical composition, we observed a higher mineral/organic content in the SDF/NaF treated dentin + pH-c groups (Stage 3) than in the positive control groups (Saforide p=0.03; Cariestop p=0.008; RivaStar p=0.013; NaF p=0.04). The XRD results showed that the crystallite size of hydroxyapatite increased in the SDF/NaF treated dentin + pH-c groups (between +63% in RivaStar to +108% in Saforide), regarding the positive control. SEM images showed that after application of the SDF/NaF products a crystalline precipitate formed on the dentin surface and partially filled the dentin tubules. The flexural strength (MPa) values were higher in the dentin treated with SDF/NaF + pH-c (Stage 3) compared to the positive control groups (Saforide p=0.002; Cariestop p=0.04; RivaStar p=0.04; NaF p=0.02). Conclusions: The application of SDF/NaF affected the physicochemical and mechanical properties of demineralized dentin. According to the results, the use of SFD/NaF had a remineralizing effect on the dentin surface even under acid challenge. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-03-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/210005 10.1590/1678-7757-2022-0306 |
url |
https://www.revistas.usp.br/jaos/article/view/210005 |
identifier_str_mv |
10.1590/1678-7757-2022-0306 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/210005/192556 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 31 (2023); e20220306 Journal of Applied Oral Science; Vol. 31 (2023); e20220306 Journal of Applied Oral Science; v. 31 (2023); e20220306 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221683529809920 |