An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells

Detalhes bibliográficos
Autor(a) principal: Kantrong, Nutthapong
Data de Publicação: 2023
Outros Autores: Kumtawee, Jittranut, Damrongrungruang, Teerasak, Puasiri, Subin, Makeudom, Anupong, Krisanaprakornkit, Suttichai, Chailertvanitkul, Pattama
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/213385
Resumo: Objective: To explore the potential for development of Thai propolis extract as a pulp capping agent to suppress pulpal inflammation from dental pulp infections. This study aimed to examine the anti-inflammatory effect of the propolis extract on the arachidonic acid pathway, activated by interleukin (IL)-1β, in cultured human dental pulp cells.  Methodology: Dental pulp cells, isolated from three freshly extracted third molars, were first characterized for their mesenchymal origin and treated with 10 ng/ml of IL-1β in the presence or absence of non-toxic concentrations of the extract from 0.08 to 1.25 mg/ml, as determined by the PrestoBlue cytotoxic assay. Total RNA was harvested and analyzed for mRNA expressions of 5-lipoxygenase (5-LOX) and cyclooxygenase-2 (COX-2). Western blot hybridization was performed to investigate COX-2 protein expression. Culture supernatants were assayed for released prostaglandin E2 levels. Immunofluorescence was conducted to determine involvement of nuclear factor-kappaB (NF-kB) in the inhibitory effect of the extract. Results: Stimulation of the pulp cells with IL-1β resulted in the activation of arachidonic acid metabolism via COX-2, but not 5-LOX. Incubation with various non-toxic concentrations of the propolis extract significantly inhibited upregulated COX-2 mRNA and protein expressions upon treatment with IL-1β (p<0.05), resulting in a significant decrease in elevated PGE2 levels (p<0.05). Nuclear translocation of the p50 and the p65 subunits of NF-kB upon treatment with IL-1β was also blocked by incubation with the extract. Conclusions: Upregulated COX-2 expression and enhanced PGE2 synthesis upon treatment with IL-1β in human dental pulp cells were suppressed by incubation with non-toxic doses of Thai propolis extract via involvement of the NF-kB activation. This extract could be therapeutically used as a pulp capping material due to its anti-inflammatory properties.
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spelling An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cellsCyclooxygenase-2Dental pulp cellsInterleukin-1PropolisProstaglandin E2Objective: To explore the potential for development of Thai propolis extract as a pulp capping agent to suppress pulpal inflammation from dental pulp infections. This study aimed to examine the anti-inflammatory effect of the propolis extract on the arachidonic acid pathway, activated by interleukin (IL)-1β, in cultured human dental pulp cells.  Methodology: Dental pulp cells, isolated from three freshly extracted third molars, were first characterized for their mesenchymal origin and treated with 10 ng/ml of IL-1β in the presence or absence of non-toxic concentrations of the extract from 0.08 to 1.25 mg/ml, as determined by the PrestoBlue cytotoxic assay. Total RNA was harvested and analyzed for mRNA expressions of 5-lipoxygenase (5-LOX) and cyclooxygenase-2 (COX-2). Western blot hybridization was performed to investigate COX-2 protein expression. Culture supernatants were assayed for released prostaglandin E2 levels. Immunofluorescence was conducted to determine involvement of nuclear factor-kappaB (NF-kB) in the inhibitory effect of the extract. Results: Stimulation of the pulp cells with IL-1β resulted in the activation of arachidonic acid metabolism via COX-2, but not 5-LOX. Incubation with various non-toxic concentrations of the propolis extract significantly inhibited upregulated COX-2 mRNA and protein expressions upon treatment with IL-1β (p<0.05), resulting in a significant decrease in elevated PGE2 levels (p<0.05). Nuclear translocation of the p50 and the p65 subunits of NF-kB upon treatment with IL-1β was also blocked by incubation with the extract. Conclusions: Upregulated COX-2 expression and enhanced PGE2 synthesis upon treatment with IL-1β in human dental pulp cells were suppressed by incubation with non-toxic doses of Thai propolis extract via involvement of the NF-kB activation. This extract could be therapeutically used as a pulp capping material due to its anti-inflammatory properties.Universidade de São Paulo. Faculdade de Odontologia de Bauru2023-06-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/21338510.1590/1678-7757-2023-0006Journal of Applied Oral Science; Vol. 31 (2023); e20230006Journal of Applied Oral Science; Vol. 31 (2023); e20230006Journal of Applied Oral Science; v. 31 (2023); e202300061678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/213385/195315Copyright (c) 2023 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessKantrong, NutthapongKumtawee, JittranutDamrongrungruang, TeerasakPuasiri, SubinMakeudom, AnupongKrisanaprakornkit, SuttichaiChailertvanitkul, Pattama2023-06-20T14:49:00Zoai:revistas.usp.br:article/213385Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2023-06-20T14:49Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells
title An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells
spellingShingle An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells
Kantrong, Nutthapong
Cyclooxygenase-2
Dental pulp cells
Interleukin-1
Propolis
Prostaglandin E2
title_short An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells
title_full An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells
title_fullStr An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells
title_full_unstemmed An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells
title_sort An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells
author Kantrong, Nutthapong
author_facet Kantrong, Nutthapong
Kumtawee, Jittranut
Damrongrungruang, Teerasak
Puasiri, Subin
Makeudom, Anupong
Krisanaprakornkit, Suttichai
Chailertvanitkul, Pattama
author_role author
author2 Kumtawee, Jittranut
Damrongrungruang, Teerasak
Puasiri, Subin
Makeudom, Anupong
Krisanaprakornkit, Suttichai
Chailertvanitkul, Pattama
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Kantrong, Nutthapong
Kumtawee, Jittranut
Damrongrungruang, Teerasak
Puasiri, Subin
Makeudom, Anupong
Krisanaprakornkit, Suttichai
Chailertvanitkul, Pattama
dc.subject.por.fl_str_mv Cyclooxygenase-2
Dental pulp cells
Interleukin-1
Propolis
Prostaglandin E2
topic Cyclooxygenase-2
Dental pulp cells
Interleukin-1
Propolis
Prostaglandin E2
description Objective: To explore the potential for development of Thai propolis extract as a pulp capping agent to suppress pulpal inflammation from dental pulp infections. This study aimed to examine the anti-inflammatory effect of the propolis extract on the arachidonic acid pathway, activated by interleukin (IL)-1β, in cultured human dental pulp cells.  Methodology: Dental pulp cells, isolated from three freshly extracted third molars, were first characterized for their mesenchymal origin and treated with 10 ng/ml of IL-1β in the presence or absence of non-toxic concentrations of the extract from 0.08 to 1.25 mg/ml, as determined by the PrestoBlue cytotoxic assay. Total RNA was harvested and analyzed for mRNA expressions of 5-lipoxygenase (5-LOX) and cyclooxygenase-2 (COX-2). Western blot hybridization was performed to investigate COX-2 protein expression. Culture supernatants were assayed for released prostaglandin E2 levels. Immunofluorescence was conducted to determine involvement of nuclear factor-kappaB (NF-kB) in the inhibitory effect of the extract. Results: Stimulation of the pulp cells with IL-1β resulted in the activation of arachidonic acid metabolism via COX-2, but not 5-LOX. Incubation with various non-toxic concentrations of the propolis extract significantly inhibited upregulated COX-2 mRNA and protein expressions upon treatment with IL-1β (p<0.05), resulting in a significant decrease in elevated PGE2 levels (p<0.05). Nuclear translocation of the p50 and the p65 subunits of NF-kB upon treatment with IL-1β was also blocked by incubation with the extract. Conclusions: Upregulated COX-2 expression and enhanced PGE2 synthesis upon treatment with IL-1β in human dental pulp cells were suppressed by incubation with non-toxic doses of Thai propolis extract via involvement of the NF-kB activation. This extract could be therapeutically used as a pulp capping material due to its anti-inflammatory properties.
publishDate 2023
dc.date.none.fl_str_mv 2023-06-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/213385
10.1590/1678-7757-2023-0006
url https://www.revistas.usp.br/jaos/article/view/213385
identifier_str_mv 10.1590/1678-7757-2023-0006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/213385/195315
dc.rights.driver.fl_str_mv Copyright (c) 2023 Journal of Applied Oral Science
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Journal of Applied Oral Science
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 31 (2023); e20230006
Journal of Applied Oral Science; Vol. 31 (2023); e20230006
Journal of Applied Oral Science; v. 31 (2023); e20230006
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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