Transcriptome profile of highly osteoblastic/cementoblastic periodontal ligament cell clones
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/190682 |
Resumo: | Heterogeneous cell populations of osteo/cementoblastic (O/C) or fibroblastic phenotypes constitute the periodontal dental ligament (PDL). A better understanding of these PDL cell subpopulations is essential to propose regenerative approaches based on a sound biological rationale. Objective: Our study aimed to clarify the differential transcriptome profile of PDL cells poised to differentiate into the O/C cell lineage. Methodology: To characterize periodontal-derived cells with distinct differentiation capacities, single-cell-derived clones were isolated from adult human PDL progenitor cells and their potential to differentiate into osteo/cementoblastic (O/C) phenotype (C-O clones) or fibroblastic phenotype (C-F clones) was assessed in vitro. The transcriptome profile of the clonal cell lines in standard medium cultivation was evaluated using next-generation sequencing technology (RNA-seq). Over 230 differentially expressed genes (DEG) were identified, in which C-O clones showed a higher number of upregulated genes (193) and 42 downregulated genes. Results: The upregulated genes were associated with the Cadherin and Wnt signaling pathways as well as annotated biological processes, including “anatomical structure development” and “cell adhesion.” Both transcriptome and RT-qPCR showed up-regulation of WNT2, WNT16, and WIF1 in C-O clones. Conclusions: This comprehensive transcriptomic assessment of human PDL progenitor cells revealed that expression of transcripts related to the biological process “anatomical structure development,” Cadherin signaling, and Wnt signaling can identify PDL cells with a higher potential to commit to the O/C phenotype. A better understanding of these pathways and their function in O/C differentiation will help to improve protocols for periodontal regenerative therapies. |
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network_name_str |
Journal of applied oral science (Online) |
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Transcriptome profile of highly osteoblastic/cementoblastic periodontal ligament cell clonesCell differentiationClone cellsRNA Sequence AnalysisPeriodontal ligamentOsteoblastsHeterogeneous cell populations of osteo/cementoblastic (O/C) or fibroblastic phenotypes constitute the periodontal dental ligament (PDL). A better understanding of these PDL cell subpopulations is essential to propose regenerative approaches based on a sound biological rationale. Objective: Our study aimed to clarify the differential transcriptome profile of PDL cells poised to differentiate into the O/C cell lineage. Methodology: To characterize periodontal-derived cells with distinct differentiation capacities, single-cell-derived clones were isolated from adult human PDL progenitor cells and their potential to differentiate into osteo/cementoblastic (O/C) phenotype (C-O clones) or fibroblastic phenotype (C-F clones) was assessed in vitro. The transcriptome profile of the clonal cell lines in standard medium cultivation was evaluated using next-generation sequencing technology (RNA-seq). Over 230 differentially expressed genes (DEG) were identified, in which C-O clones showed a higher number of upregulated genes (193) and 42 downregulated genes. Results: The upregulated genes were associated with the Cadherin and Wnt signaling pathways as well as annotated biological processes, including “anatomical structure development” and “cell adhesion.” Both transcriptome and RT-qPCR showed up-regulation of WNT2, WNT16, and WIF1 in C-O clones. Conclusions: This comprehensive transcriptomic assessment of human PDL progenitor cells revealed that expression of transcripts related to the biological process “anatomical structure development,” Cadherin signaling, and Wnt signaling can identify PDL cells with a higher potential to commit to the O/C phenotype. A better understanding of these pathways and their function in O/C differentiation will help to improve protocols for periodontal regenerative therapies.Universidade de São Paulo. Faculdade de Odontologia de Bauru2021-09-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/19068210.1590/1678-7757-2020-0242Journal of Applied Oral Science; Vol. 28 (2020); e20200242Journal of Applied Oral Science; Vol. 28 (2020); e20200242Journal of Applied Oral Science; v. 28 (2020); e202002421678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/190682/175884Copyright (c) 2021 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSaito, Miki TaketomiMofatto, Luciana Souto Albiero, Mayra LainoCasati, Márcio ZafallonSallum, Enilson AntonioNociti Junior, Francisco Humberto Silvério, Karina Gonzales2021-09-15T17:43:15Zoai:revistas.usp.br:article/190682Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2021-09-15T17:43:15Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Transcriptome profile of highly osteoblastic/cementoblastic periodontal ligament cell clones |
title |
Transcriptome profile of highly osteoblastic/cementoblastic periodontal ligament cell clones |
spellingShingle |
Transcriptome profile of highly osteoblastic/cementoblastic periodontal ligament cell clones Saito, Miki Taketomi Cell differentiation Clone cells RNA Sequence Analysis Periodontal ligament Osteoblasts |
title_short |
Transcriptome profile of highly osteoblastic/cementoblastic periodontal ligament cell clones |
title_full |
Transcriptome profile of highly osteoblastic/cementoblastic periodontal ligament cell clones |
title_fullStr |
Transcriptome profile of highly osteoblastic/cementoblastic periodontal ligament cell clones |
title_full_unstemmed |
Transcriptome profile of highly osteoblastic/cementoblastic periodontal ligament cell clones |
title_sort |
Transcriptome profile of highly osteoblastic/cementoblastic periodontal ligament cell clones |
author |
Saito, Miki Taketomi |
author_facet |
Saito, Miki Taketomi Mofatto, Luciana Souto Albiero, Mayra Laino Casati, Márcio Zafallon Sallum, Enilson Antonio Nociti Junior, Francisco Humberto Silvério, Karina Gonzales |
author_role |
author |
author2 |
Mofatto, Luciana Souto Albiero, Mayra Laino Casati, Márcio Zafallon Sallum, Enilson Antonio Nociti Junior, Francisco Humberto Silvério, Karina Gonzales |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Saito, Miki Taketomi Mofatto, Luciana Souto Albiero, Mayra Laino Casati, Márcio Zafallon Sallum, Enilson Antonio Nociti Junior, Francisco Humberto Silvério, Karina Gonzales |
dc.subject.por.fl_str_mv |
Cell differentiation Clone cells RNA Sequence Analysis Periodontal ligament Osteoblasts |
topic |
Cell differentiation Clone cells RNA Sequence Analysis Periodontal ligament Osteoblasts |
description |
Heterogeneous cell populations of osteo/cementoblastic (O/C) or fibroblastic phenotypes constitute the periodontal dental ligament (PDL). A better understanding of these PDL cell subpopulations is essential to propose regenerative approaches based on a sound biological rationale. Objective: Our study aimed to clarify the differential transcriptome profile of PDL cells poised to differentiate into the O/C cell lineage. Methodology: To characterize periodontal-derived cells with distinct differentiation capacities, single-cell-derived clones were isolated from adult human PDL progenitor cells and their potential to differentiate into osteo/cementoblastic (O/C) phenotype (C-O clones) or fibroblastic phenotype (C-F clones) was assessed in vitro. The transcriptome profile of the clonal cell lines in standard medium cultivation was evaluated using next-generation sequencing technology (RNA-seq). Over 230 differentially expressed genes (DEG) were identified, in which C-O clones showed a higher number of upregulated genes (193) and 42 downregulated genes. Results: The upregulated genes were associated with the Cadherin and Wnt signaling pathways as well as annotated biological processes, including “anatomical structure development” and “cell adhesion.” Both transcriptome and RT-qPCR showed up-regulation of WNT2, WNT16, and WIF1 in C-O clones. Conclusions: This comprehensive transcriptomic assessment of human PDL progenitor cells revealed that expression of transcripts related to the biological process “anatomical structure development,” Cadherin signaling, and Wnt signaling can identify PDL cells with a higher potential to commit to the O/C phenotype. A better understanding of these pathways and their function in O/C differentiation will help to improve protocols for periodontal regenerative therapies. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/190682 10.1590/1678-7757-2020-0242 |
url |
https://www.revistas.usp.br/jaos/article/view/190682 |
identifier_str_mv |
10.1590/1678-7757-2020-0242 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/190682/175884 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 28 (2020); e20200242 Journal of Applied Oral Science; Vol. 28 (2020); e20200242 Journal of Applied Oral Science; v. 28 (2020); e20200242 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221682033491968 |