Caries-free subjects have high levels of urease and arginine deiminase activity
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572014000300235 |
Resumo: | Objectives: This study investigated the relationship between urease and arginine deiminase system (ADS) activities and dental caries through a cross-sectional study. Material and Methods: Urease and ADS activities were measured in saliva and plaque samples from 10 caries-free subjects and 13 caries-active. Urease activity was obtained from the ammonia produced by incubation of plaque and saliva samples in urea. ADS activity was obtained from the ammonia generated by the arginine-HCl and Tris-maleate buffer. Specific activity was defined as micromoles of ammonia per minute per milligram of protein. Shapiro-Wilk statistical test was used to analyze the distribution of the data, and Mann-Whitney test was used to determine the significance of the data. Results: The specific urease activity in saliva and plaque was significantly higher in individuals with low DMFT scores. ADS activity in saliva (6.050 vs 1.350, p=0.0154) and plaque (8.830 vs 1.210, p=0.025) was also higher in individuals with low DMFT scores. Conclusions: Caries-free subjects had a higher ammonia generation activity by urease and arginine deiminase system for both saliva and plaque samples than low caries-active subjects. High levels of alkali production in oral environment were related to caries-free subjects. |
id |
USP-17_c918d736a499d33395c2a41e7c75f026 |
---|---|
oai_identifier_str |
oai:scielo:S1678-77572014000300235 |
network_acronym_str |
USP-17 |
network_name_str |
Journal of applied oral science (Online) |
repository_id_str |
|
spelling |
Caries-free subjects have high levels of urease and arginine deiminase activityAlkaliArginineDental plaqueSalivaUreaseDental caries Objectives: This study investigated the relationship between urease and arginine deiminase system (ADS) activities and dental caries through a cross-sectional study. Material and Methods: Urease and ADS activities were measured in saliva and plaque samples from 10 caries-free subjects and 13 caries-active. Urease activity was obtained from the ammonia produced by incubation of plaque and saliva samples in urea. ADS activity was obtained from the ammonia generated by the arginine-HCl and Tris-maleate buffer. Specific activity was defined as micromoles of ammonia per minute per milligram of protein. Shapiro-Wilk statistical test was used to analyze the distribution of the data, and Mann-Whitney test was used to determine the significance of the data. Results: The specific urease activity in saliva and plaque was significantly higher in individuals with low DMFT scores. ADS activity in saliva (6.050 vs 1.350, p=0.0154) and plaque (8.830 vs 1.210, p=0.025) was also higher in individuals with low DMFT scores. Conclusions: Caries-free subjects had a higher ammonia generation activity by urease and arginine deiminase system for both saliva and plaque samples than low caries-active subjects. High levels of alkali production in oral environment were related to caries-free subjects. Faculdade De Odontologia De Bauru - USP2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572014000300235Journal of Applied Oral Science v.22 n.3 2014reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-775720130591info:eu-repo/semantics/openAccessREYES,EvelynMARTIN,JavierMONCADA,GustavoNEIRA,MiguelPALMA,PatriciaGORDAN,ValeriaOYARZO,Juan FernandoYEVENES,Ismaeleng2014-06-13T00:00:00Zoai:scielo:S1678-77572014000300235Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2014-06-13T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Caries-free subjects have high levels of urease and arginine deiminase activity |
title |
Caries-free subjects have high levels of urease and arginine deiminase activity |
spellingShingle |
Caries-free subjects have high levels of urease and arginine deiminase activity REYES,Evelyn Alkali Arginine Dental plaque Saliva Urease Dental caries |
title_short |
Caries-free subjects have high levels of urease and arginine deiminase activity |
title_full |
Caries-free subjects have high levels of urease and arginine deiminase activity |
title_fullStr |
Caries-free subjects have high levels of urease and arginine deiminase activity |
title_full_unstemmed |
Caries-free subjects have high levels of urease and arginine deiminase activity |
title_sort |
Caries-free subjects have high levels of urease and arginine deiminase activity |
author |
REYES,Evelyn |
author_facet |
REYES,Evelyn MARTIN,Javier MONCADA,Gustavo NEIRA,Miguel PALMA,Patricia GORDAN,Valeria OYARZO,Juan Fernando YEVENES,Ismael |
author_role |
author |
author2 |
MARTIN,Javier MONCADA,Gustavo NEIRA,Miguel PALMA,Patricia GORDAN,Valeria OYARZO,Juan Fernando YEVENES,Ismael |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
REYES,Evelyn MARTIN,Javier MONCADA,Gustavo NEIRA,Miguel PALMA,Patricia GORDAN,Valeria OYARZO,Juan Fernando YEVENES,Ismael |
dc.subject.por.fl_str_mv |
Alkali Arginine Dental plaque Saliva Urease Dental caries |
topic |
Alkali Arginine Dental plaque Saliva Urease Dental caries |
description |
Objectives: This study investigated the relationship between urease and arginine deiminase system (ADS) activities and dental caries through a cross-sectional study. Material and Methods: Urease and ADS activities were measured in saliva and plaque samples from 10 caries-free subjects and 13 caries-active. Urease activity was obtained from the ammonia produced by incubation of plaque and saliva samples in urea. ADS activity was obtained from the ammonia generated by the arginine-HCl and Tris-maleate buffer. Specific activity was defined as micromoles of ammonia per minute per milligram of protein. Shapiro-Wilk statistical test was used to analyze the distribution of the data, and Mann-Whitney test was used to determine the significance of the data. Results: The specific urease activity in saliva and plaque was significantly higher in individuals with low DMFT scores. ADS activity in saliva (6.050 vs 1.350, p=0.0154) and plaque (8.830 vs 1.210, p=0.025) was also higher in individuals with low DMFT scores. Conclusions: Caries-free subjects had a higher ammonia generation activity by urease and arginine deiminase system for both saliva and plaque samples than low caries-active subjects. High levels of alkali production in oral environment were related to caries-free subjects. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572014000300235 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572014000300235 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-775720130591 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science v.22 n.3 2014 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1748936438104195072 |