Bioactivity, physical and chemical properties of MTA mixed with propylene glycol

Detalhes bibliográficos
Autor(a) principal: NATU, Vaishali Prakash
Data de Publicação: 2015
Outros Autores: DUBEY, Nileshkumar, LOKE, Gerald Choon Leong, TAN, Teng Seng, NG, Wee Hsuan, YONG, Chee Weng, CAO, Tong, ROSA, Vinicius
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/104930
Resumo: AbstractObjective To investigate the physical (setting time, hardness, flowability, microstructure) and chemical (pH change, calcium release, crystallinity) properties and the biological outcomes (cell survival and differentiation) of mineral trioxide aggregate (MTA) mixed using different proportions of propylene glycol (PG) and water.Material and Methods White MTA was mixed with different water/PG ratios (100/0, 80/20 and 50/50). Composition (XRD), microstructure (SEM), setting time (ASTM C266-13), flowability (ANSI/ADA 57-2000), Knoop hardness (100 g/10 s) and chemical characteristics (pH change and Ca2+ release for 7 days) were evaluated. Cell proliferation, osteo/odontoblastic gene expression and mineralization induced by MTA mixed with PG were evaluated. MTA discs (5 mm in diameter, 2 mm thick) were prepared and soaked in culture medium for 7 days. Next, the discs were removed and the medium used to culture dental pulp stem cells (DPSC) for 28 days. Cells survival was evaluated using MTS assay (24, 72 and 120 h) and differentiation with RT-PCR (ALP, OCN, Runx2, DSPP and MEPE) and alizarin red staining (7 and 14 days). Data were analysed using one-way ANOVA and Tukey’s post-hoc analysis (a=0.05).Results The addition of PG significantly increased setting time, flowability and Ca2+ release, but it compromised the hardness of the material. SEM showed that 50/50 group resulted porous material after setting due to the incomplete setting reaction, as shown by XRD analysis. The addition of PG (80/20 and 50/50) was not capable to improve cell proliferation or to enhance gene expression, and mineralized deposition of DPSC after 7 and 14 days as compared to the 100/0.Conclusion Except for flowability, the addition of PG did not promote further improvements on the chemical and physical properties evaluated, and it was not capable of enhancing the bioactivity of the MTA.
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spelling Bioactivity, physical and chemical properties of MTA mixed with propylene glycol AbstractObjective To investigate the physical (setting time, hardness, flowability, microstructure) and chemical (pH change, calcium release, crystallinity) properties and the biological outcomes (cell survival and differentiation) of mineral trioxide aggregate (MTA) mixed using different proportions of propylene glycol (PG) and water.Material and Methods White MTA was mixed with different water/PG ratios (100/0, 80/20 and 50/50). Composition (XRD), microstructure (SEM), setting time (ASTM C266-13), flowability (ANSI/ADA 57-2000), Knoop hardness (100 g/10 s) and chemical characteristics (pH change and Ca2+ release for 7 days) were evaluated. Cell proliferation, osteo/odontoblastic gene expression and mineralization induced by MTA mixed with PG were evaluated. MTA discs (5 mm in diameter, 2 mm thick) were prepared and soaked in culture medium for 7 days. Next, the discs were removed and the medium used to culture dental pulp stem cells (DPSC) for 28 days. Cells survival was evaluated using MTS assay (24, 72 and 120 h) and differentiation with RT-PCR (ALP, OCN, Runx2, DSPP and MEPE) and alizarin red staining (7 and 14 days). Data were analysed using one-way ANOVA and Tukey’s post-hoc analysis (a=0.05).Results The addition of PG significantly increased setting time, flowability and Ca2+ release, but it compromised the hardness of the material. SEM showed that 50/50 group resulted porous material after setting due to the incomplete setting reaction, as shown by XRD analysis. The addition of PG (80/20 and 50/50) was not capable to improve cell proliferation or to enhance gene expression, and mineralized deposition of DPSC after 7 and 14 days as compared to the 100/0.Conclusion Except for flowability, the addition of PG did not promote further improvements on the chemical and physical properties evaluated, and it was not capable of enhancing the bioactivity of the MTA. Universidade de São Paulo. Faculdade de Odontologia de Bauru2015-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/10493010.1590/1678-775720150084Journal of Applied Oral Science; Vol. 23 No. 4 (2015); 405-411Journal of Applied Oral Science; Vol. 23 Núm. 4 (2015); 405-411Journal of Applied Oral Science; v. 23 n. 4 (2015); 405-4111678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/104930/103721Copyright (c) 2015 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessNATU, Vaishali PrakashDUBEY, NileshkumarLOKE, Gerald Choon LeongTAN, Teng SengNG, Wee HsuanYONG, Chee WengCAO, TongROSA, Vinicius2015-09-25T18:45:40Zoai:revistas.usp.br:article/104930Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2015-09-25T18:45:40Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Bioactivity, physical and chemical properties of MTA mixed with propylene glycol
title Bioactivity, physical and chemical properties of MTA mixed with propylene glycol
spellingShingle Bioactivity, physical and chemical properties of MTA mixed with propylene glycol
NATU, Vaishali Prakash
title_short Bioactivity, physical and chemical properties of MTA mixed with propylene glycol
title_full Bioactivity, physical and chemical properties of MTA mixed with propylene glycol
title_fullStr Bioactivity, physical and chemical properties of MTA mixed with propylene glycol
title_full_unstemmed Bioactivity, physical and chemical properties of MTA mixed with propylene glycol
title_sort Bioactivity, physical and chemical properties of MTA mixed with propylene glycol
author NATU, Vaishali Prakash
author_facet NATU, Vaishali Prakash
DUBEY, Nileshkumar
LOKE, Gerald Choon Leong
TAN, Teng Seng
NG, Wee Hsuan
YONG, Chee Weng
CAO, Tong
ROSA, Vinicius
author_role author
author2 DUBEY, Nileshkumar
LOKE, Gerald Choon Leong
TAN, Teng Seng
NG, Wee Hsuan
YONG, Chee Weng
CAO, Tong
ROSA, Vinicius
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv NATU, Vaishali Prakash
DUBEY, Nileshkumar
LOKE, Gerald Choon Leong
TAN, Teng Seng
NG, Wee Hsuan
YONG, Chee Weng
CAO, Tong
ROSA, Vinicius
description AbstractObjective To investigate the physical (setting time, hardness, flowability, microstructure) and chemical (pH change, calcium release, crystallinity) properties and the biological outcomes (cell survival and differentiation) of mineral trioxide aggregate (MTA) mixed using different proportions of propylene glycol (PG) and water.Material and Methods White MTA was mixed with different water/PG ratios (100/0, 80/20 and 50/50). Composition (XRD), microstructure (SEM), setting time (ASTM C266-13), flowability (ANSI/ADA 57-2000), Knoop hardness (100 g/10 s) and chemical characteristics (pH change and Ca2+ release for 7 days) were evaluated. Cell proliferation, osteo/odontoblastic gene expression and mineralization induced by MTA mixed with PG were evaluated. MTA discs (5 mm in diameter, 2 mm thick) were prepared and soaked in culture medium for 7 days. Next, the discs were removed and the medium used to culture dental pulp stem cells (DPSC) for 28 days. Cells survival was evaluated using MTS assay (24, 72 and 120 h) and differentiation with RT-PCR (ALP, OCN, Runx2, DSPP and MEPE) and alizarin red staining (7 and 14 days). Data were analysed using one-way ANOVA and Tukey’s post-hoc analysis (a=0.05).Results The addition of PG significantly increased setting time, flowability and Ca2+ release, but it compromised the hardness of the material. SEM showed that 50/50 group resulted porous material after setting due to the incomplete setting reaction, as shown by XRD analysis. The addition of PG (80/20 and 50/50) was not capable to improve cell proliferation or to enhance gene expression, and mineralized deposition of DPSC after 7 and 14 days as compared to the 100/0.Conclusion Except for flowability, the addition of PG did not promote further improvements on the chemical and physical properties evaluated, and it was not capable of enhancing the bioactivity of the MTA.
publishDate 2015
dc.date.none.fl_str_mv 2015-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/104930
10.1590/1678-775720150084
url https://www.revistas.usp.br/jaos/article/view/104930
identifier_str_mv 10.1590/1678-775720150084
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/104930/103721
dc.rights.driver.fl_str_mv Copyright (c) 2015 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2015 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 23 No. 4 (2015); 405-411
Journal of Applied Oral Science; Vol. 23 Núm. 4 (2015); 405-411
Journal of Applied Oral Science; v. 23 n. 4 (2015); 405-411
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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