Comparative scanning electron microscopic study of the effect of different dental conditioners on dentin micromorphology
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/3547 |
Resumo: | This study evaluated comparatively by scanning electron microscopy (SEM) the effect of different dental conditioners on dentin micromorphology, when used according to the same protocol. Forty dentin sticks were obtained from 20 caries-free third human molars and were assigned to 4 groups corresponding to 3 conditioners (phosphoric acid 37%, Clearfil SE Bond and iBond) and an untreated control group. After application of the conditioners, the specimens were immersed in 50% ethanol solution during 10 s, chemically fixed and dehydrated to prepare them to SEM analysis. In the control group, dentin surface was completely covered by smear layer and all dentinal tubules were occluded. In the phosphoric acid-etched group, dentin surface was completely clean and presented exposed dentinal tubule openings; this was the only group in which the tubules exhibited the funnel-shaped aspect. In the groups conditioned with Clearfil SE Bond primer and iBond, which are less acidic than phosphoric acid, tubule openings were occluded or partially occluded, though smear layer removal was observed. SE Bond was more efficient in removing the smear layer than iBond. In the Clearfil SE Bond group, the cuff-like aspect of peritubular dentin was more evident. It may be concluded all tested conditioners were able to change dentin morphology. However, it cannot be stated that the agent aggressiveness was the only cause of the micromorphological alterations because a single morphological pattern was not established for each group, but rather an association of different aspects, according to the aggressiveness of the tested conditioner. |
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Journal of applied oral science (Online) |
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Comparative scanning electron microscopic study of the effect of different dental conditioners on dentin micromorphology Adhesive systemSelf-etching adhesivesDental conditioners This study evaluated comparatively by scanning electron microscopy (SEM) the effect of different dental conditioners on dentin micromorphology, when used according to the same protocol. Forty dentin sticks were obtained from 20 caries-free third human molars and were assigned to 4 groups corresponding to 3 conditioners (phosphoric acid 37%, Clearfil SE Bond and iBond) and an untreated control group. After application of the conditioners, the specimens were immersed in 50% ethanol solution during 10 s, chemically fixed and dehydrated to prepare them to SEM analysis. In the control group, dentin surface was completely covered by smear layer and all dentinal tubules were occluded. In the phosphoric acid-etched group, dentin surface was completely clean and presented exposed dentinal tubule openings; this was the only group in which the tubules exhibited the funnel-shaped aspect. In the groups conditioned with Clearfil SE Bond primer and iBond, which are less acidic than phosphoric acid, tubule openings were occluded or partially occluded, though smear layer removal was observed. SE Bond was more efficient in removing the smear layer than iBond. In the Clearfil SE Bond group, the cuff-like aspect of peritubular dentin was more evident. It may be concluded all tested conditioners were able to change dentin morphology. However, it cannot be stated that the agent aggressiveness was the only cause of the micromorphological alterations because a single morphological pattern was not established for each group, but rather an association of different aspects, according to the aggressiveness of the tested conditioner. Universidade de São Paulo. Faculdade de Odontologia de Bauru2008-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/354710.1590/S1678-77572008000200004Journal of Applied Oral Science; Vol. 16 No. 2 (2008); 100-105 Journal of Applied Oral Science; Vol. 16 Núm. 2 (2008); 100-105 Journal of Applied Oral Science; v. 16 n. 2 (2008); 100-105 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3547/4237Copyright (c) 2008 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessSusin, Alexandre HenriqueAlves, Luana SeveroMelo, Gliciana Piovesan deLenzi, Tathiane Larissa2012-04-27T12:00:14Zoai:revistas.usp.br:article/3547Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T12:00:14Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Comparative scanning electron microscopic study of the effect of different dental conditioners on dentin micromorphology |
title |
Comparative scanning electron microscopic study of the effect of different dental conditioners on dentin micromorphology |
spellingShingle |
Comparative scanning electron microscopic study of the effect of different dental conditioners on dentin micromorphology Susin, Alexandre Henrique Adhesive system Self-etching adhesives Dental conditioners |
title_short |
Comparative scanning electron microscopic study of the effect of different dental conditioners on dentin micromorphology |
title_full |
Comparative scanning electron microscopic study of the effect of different dental conditioners on dentin micromorphology |
title_fullStr |
Comparative scanning electron microscopic study of the effect of different dental conditioners on dentin micromorphology |
title_full_unstemmed |
Comparative scanning electron microscopic study of the effect of different dental conditioners on dentin micromorphology |
title_sort |
Comparative scanning electron microscopic study of the effect of different dental conditioners on dentin micromorphology |
author |
Susin, Alexandre Henrique |
author_facet |
Susin, Alexandre Henrique Alves, Luana Severo Melo, Gliciana Piovesan de Lenzi, Tathiane Larissa |
author_role |
author |
author2 |
Alves, Luana Severo Melo, Gliciana Piovesan de Lenzi, Tathiane Larissa |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Susin, Alexandre Henrique Alves, Luana Severo Melo, Gliciana Piovesan de Lenzi, Tathiane Larissa |
dc.subject.por.fl_str_mv |
Adhesive system Self-etching adhesives Dental conditioners |
topic |
Adhesive system Self-etching adhesives Dental conditioners |
description |
This study evaluated comparatively by scanning electron microscopy (SEM) the effect of different dental conditioners on dentin micromorphology, when used according to the same protocol. Forty dentin sticks were obtained from 20 caries-free third human molars and were assigned to 4 groups corresponding to 3 conditioners (phosphoric acid 37%, Clearfil SE Bond and iBond) and an untreated control group. After application of the conditioners, the specimens were immersed in 50% ethanol solution during 10 s, chemically fixed and dehydrated to prepare them to SEM analysis. In the control group, dentin surface was completely covered by smear layer and all dentinal tubules were occluded. In the phosphoric acid-etched group, dentin surface was completely clean and presented exposed dentinal tubule openings; this was the only group in which the tubules exhibited the funnel-shaped aspect. In the groups conditioned with Clearfil SE Bond primer and iBond, which are less acidic than phosphoric acid, tubule openings were occluded or partially occluded, though smear layer removal was observed. SE Bond was more efficient in removing the smear layer than iBond. In the Clearfil SE Bond group, the cuff-like aspect of peritubular dentin was more evident. It may be concluded all tested conditioners were able to change dentin morphology. However, it cannot be stated that the agent aggressiveness was the only cause of the micromorphological alterations because a single morphological pattern was not established for each group, but rather an association of different aspects, according to the aggressiveness of the tested conditioner. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3547 10.1590/S1678-77572008000200004 |
url |
https://www.revistas.usp.br/jaos/article/view/3547 |
identifier_str_mv |
10.1590/S1678-77572008000200004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3547/4237 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2008 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2008 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 16 No. 2 (2008); 100-105 Journal of Applied Oral Science; Vol. 16 Núm. 2 (2008); 100-105 Journal of Applied Oral Science; v. 16 n. 2 (2008); 100-105 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221673910173696 |