Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/199687 |
Resumo: | Purpose: To compare, both qualitatively and quantitatively, the inflammatory cells, vascular density and IL-6 immunolabeled cells present in the pulp after pulpotomy with white MTA versus 15.5% ferric sulfate (FS). Methodology: Forty-eight mandibular first molars from 24 Wistar rats were divided into MTA or FS groups and subdivided according to the period after pulpotomy procedure (24, 48 and 72 hours). Four teeth (sound and untreated) were used as controls. Histological sections were obtained and assessed through the descriptive analysis of morphological aspects of pulp tissue and the quantification of inflammatory cells, vascular density and interleukin-6 (IL-6) expression. Data were statistically analyzed (p<0.05). Results: The number of inflammatory cells was similar in both groups, being predominantly localized at the cervical radicular third. In the MTA group, increased inflammation was observed at 48 hours. Vascular density was similar in both groups and over time, being predominant in the medium radicular third. No correlation was found between the number of inflammatory cells and the vascular density. Pulp tissue was more organized in MTA-treated teeth. In both groups, a weak to moderate IL-6 expression was detected in odontoblasts and inflammatory cells. Comparing both groups, there was a greater IL-6 expression in the cervical radicular third of teeth treated with MTA at 24 hours and in the medium and apical thirds at 72 hours, while in the FS group a greater IL-6 expression was found in the apical third at 24 hours. Conclusion: The MTA group presented better histological features and greater IL-6 expression than the FS group. However, no difference was observed between the groups regarding the inflammatory status and vascularization, suggesting the usefulness of FS as a low-cost alternative to MTA. |
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USP-17 |
network_name_str |
Journal of applied oral science (Online) |
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Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfateMaterials testingFerric sulfatePulpotomyInterleukin 6Purpose: To compare, both qualitatively and quantitatively, the inflammatory cells, vascular density and IL-6 immunolabeled cells present in the pulp after pulpotomy with white MTA versus 15.5% ferric sulfate (FS). Methodology: Forty-eight mandibular first molars from 24 Wistar rats were divided into MTA or FS groups and subdivided according to the period after pulpotomy procedure (24, 48 and 72 hours). Four teeth (sound and untreated) were used as controls. Histological sections were obtained and assessed through the descriptive analysis of morphological aspects of pulp tissue and the quantification of inflammatory cells, vascular density and interleukin-6 (IL-6) expression. Data were statistically analyzed (p<0.05). Results: The number of inflammatory cells was similar in both groups, being predominantly localized at the cervical radicular third. In the MTA group, increased inflammation was observed at 48 hours. Vascular density was similar in both groups and over time, being predominant in the medium radicular third. No correlation was found between the number of inflammatory cells and the vascular density. Pulp tissue was more organized in MTA-treated teeth. In both groups, a weak to moderate IL-6 expression was detected in odontoblasts and inflammatory cells. Comparing both groups, there was a greater IL-6 expression in the cervical radicular third of teeth treated with MTA at 24 hours and in the medium and apical thirds at 72 hours, while in the FS group a greater IL-6 expression was found in the apical third at 24 hours. Conclusion: The MTA group presented better histological features and greater IL-6 expression than the FS group. However, no difference was observed between the groups regarding the inflammatory status and vascularization, suggesting the usefulness of FS as a low-cost alternative to MTA.Universidade de São Paulo. Faculdade de Odontologia de Bauru2022-08-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/19968710.1590/1678-7757-2018-0550Journal of Applied Oral Science; Vol. 27 (2019); e20180550Journal of Applied Oral Science; Vol. 27 (2019); e20180550Journal of Applied Oral Science; v. 27 (2019); e201805501678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/199687/184949Copyright (c) 2022 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSakai, Vivien ThiemyNeves, Juliana dos SantosMartins, Natália SilvaOliveira, Thais MarchiniGarbelini, Cássia Cilene DezanPegoraro, Camila de Oliveira RodiniCosme-Silva, LeopoldoJunqueira, Marina AzevedoLopes, Camila Soares2022-07-01T13:02:41Zoai:revistas.usp.br:article/199687Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2022-07-01T13:02:41Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate |
title |
Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate |
spellingShingle |
Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate Sakai, Vivien Thiemy Materials testing Ferric sulfate Pulpotomy Interleukin 6 |
title_short |
Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate |
title_full |
Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate |
title_fullStr |
Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate |
title_full_unstemmed |
Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate |
title_sort |
Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate |
author |
Sakai, Vivien Thiemy |
author_facet |
Sakai, Vivien Thiemy Neves, Juliana dos Santos Martins, Natália Silva Oliveira, Thais Marchini Garbelini, Cássia Cilene Dezan Pegoraro, Camila de Oliveira Rodini Cosme-Silva, Leopoldo Junqueira, Marina Azevedo Lopes, Camila Soares |
author_role |
author |
author2 |
Neves, Juliana dos Santos Martins, Natália Silva Oliveira, Thais Marchini Garbelini, Cássia Cilene Dezan Pegoraro, Camila de Oliveira Rodini Cosme-Silva, Leopoldo Junqueira, Marina Azevedo Lopes, Camila Soares |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Sakai, Vivien Thiemy Neves, Juliana dos Santos Martins, Natália Silva Oliveira, Thais Marchini Garbelini, Cássia Cilene Dezan Pegoraro, Camila de Oliveira Rodini Cosme-Silva, Leopoldo Junqueira, Marina Azevedo Lopes, Camila Soares |
dc.subject.por.fl_str_mv |
Materials testing Ferric sulfate Pulpotomy Interleukin 6 |
topic |
Materials testing Ferric sulfate Pulpotomy Interleukin 6 |
description |
Purpose: To compare, both qualitatively and quantitatively, the inflammatory cells, vascular density and IL-6 immunolabeled cells present in the pulp after pulpotomy with white MTA versus 15.5% ferric sulfate (FS). Methodology: Forty-eight mandibular first molars from 24 Wistar rats were divided into MTA or FS groups and subdivided according to the period after pulpotomy procedure (24, 48 and 72 hours). Four teeth (sound and untreated) were used as controls. Histological sections were obtained and assessed through the descriptive analysis of morphological aspects of pulp tissue and the quantification of inflammatory cells, vascular density and interleukin-6 (IL-6) expression. Data were statistically analyzed (p<0.05). Results: The number of inflammatory cells was similar in both groups, being predominantly localized at the cervical radicular third. In the MTA group, increased inflammation was observed at 48 hours. Vascular density was similar in both groups and over time, being predominant in the medium radicular third. No correlation was found between the number of inflammatory cells and the vascular density. Pulp tissue was more organized in MTA-treated teeth. In both groups, a weak to moderate IL-6 expression was detected in odontoblasts and inflammatory cells. Comparing both groups, there was a greater IL-6 expression in the cervical radicular third of teeth treated with MTA at 24 hours and in the medium and apical thirds at 72 hours, while in the FS group a greater IL-6 expression was found in the apical third at 24 hours. Conclusion: The MTA group presented better histological features and greater IL-6 expression than the FS group. However, no difference was observed between the groups regarding the inflammatory status and vascularization, suggesting the usefulness of FS as a low-cost alternative to MTA. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08-02 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/199687 10.1590/1678-7757-2018-0550 |
url |
https://www.revistas.usp.br/jaos/article/view/199687 |
identifier_str_mv |
10.1590/1678-7757-2018-0550 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/199687/184949 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 27 (2019); e20180550 Journal of Applied Oral Science; Vol. 27 (2019); e20180550 Journal of Applied Oral Science; v. 27 (2019); e20180550 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221682618597376 |