Evolution of mirror-image pain in temporomandibular joint osteoarthritis mouse model
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/187119 |
Resumo: | Mirror-image pain is a kind of pain that occurs on the contralateral side, but its pathogenesis remains unclear. Objective: To develop an osteoarthritis mouse model for investigating mirror-image pain through observing nocifensive behaviors, histological changes, and nociceptive activity at days 3, 7, 14, 21, and 28 after the chemical induction of unilateral temporomandibular joint (TMJ) osteoarthritis. Methodology: We randomly divided 6-week-old mice into sham and complete Freund adjuvant groups. To induce nocifensive behaviors, we applied 0.04 g of von Frey filament, 10 psi of air puff, and cold acetone on both sides of whisker pads at different days. The histology of TMJ on both sides was observed by hematoxylin/eosin staining and microcomputed tomography scanning. Furthermore, the nociceptive activity was evaluated using the phosphorylated cyclic AMP response element binding protein (pCREB) and a microglia marker at different days in the trigeminal subnucleus caudalis. Results: Nocifensive behaviors against mechanical and temperature stimuli on the contralateral side became stronger than the baseline on day 28, in agreement with the elevation of the pCREB and the microglia marker in the trigeminal subnucleus caudalis. Thus, hypernociception on the contralateral side occurred at day 28. Conclusions: Clearly, the TMJ model with unilateral osteoarthritis exhibited mirror-image pain. Therefore, this model is useful in investigating the pathogenesis of pain and in developing treatments. |
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Journal of applied oral science (Online) |
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Evolution of mirror-image pain in temporomandibular joint osteoarthritis mouse modelMirror-image painOsteoarthritisComplete Freund adjuvantTemporomandibular jointNocifensive behaviorsMirror-image pain is a kind of pain that occurs on the contralateral side, but its pathogenesis remains unclear. Objective: To develop an osteoarthritis mouse model for investigating mirror-image pain through observing nocifensive behaviors, histological changes, and nociceptive activity at days 3, 7, 14, 21, and 28 after the chemical induction of unilateral temporomandibular joint (TMJ) osteoarthritis. Methodology: We randomly divided 6-week-old mice into sham and complete Freund adjuvant groups. To induce nocifensive behaviors, we applied 0.04 g of von Frey filament, 10 psi of air puff, and cold acetone on both sides of whisker pads at different days. The histology of TMJ on both sides was observed by hematoxylin/eosin staining and microcomputed tomography scanning. Furthermore, the nociceptive activity was evaluated using the phosphorylated cyclic AMP response element binding protein (pCREB) and a microglia marker at different days in the trigeminal subnucleus caudalis. Results: Nocifensive behaviors against mechanical and temperature stimuli on the contralateral side became stronger than the baseline on day 28, in agreement with the elevation of the pCREB and the microglia marker in the trigeminal subnucleus caudalis. Thus, hypernociception on the contralateral side occurred at day 28. Conclusions: Clearly, the TMJ model with unilateral osteoarthritis exhibited mirror-image pain. Therefore, this model is useful in investigating the pathogenesis of pain and in developing treatments.Universidade de São Paulo. Faculdade de Odontologia de Bauru2021-06-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/18711910.1590/1678-7757-2020-0575 Journal of Applied Oral Science; Vol. 29 (2021); e20200575Journal of Applied Oral Science; Vol. 29 (2021); e20200575Journal of Applied Oral Science; v. 29 (2021); e202005751678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/187119/172870Copyright (c) 2021 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRotpenpian, Nattapon Tapechum, Sompol Vattarakorn, AnchaleeChindasri, WongsathitCare, ChitPakaprot, NarawutWanasuntronwong, Aree2021-06-14T18:07:40Zoai:revistas.usp.br:article/187119Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2021-06-14T18:07:40Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Evolution of mirror-image pain in temporomandibular joint osteoarthritis mouse model |
title |
Evolution of mirror-image pain in temporomandibular joint osteoarthritis mouse model |
spellingShingle |
Evolution of mirror-image pain in temporomandibular joint osteoarthritis mouse model Rotpenpian, Nattapon Mirror-image pain Osteoarthritis Complete Freund adjuvant Temporomandibular joint Nocifensive behaviors |
title_short |
Evolution of mirror-image pain in temporomandibular joint osteoarthritis mouse model |
title_full |
Evolution of mirror-image pain in temporomandibular joint osteoarthritis mouse model |
title_fullStr |
Evolution of mirror-image pain in temporomandibular joint osteoarthritis mouse model |
title_full_unstemmed |
Evolution of mirror-image pain in temporomandibular joint osteoarthritis mouse model |
title_sort |
Evolution of mirror-image pain in temporomandibular joint osteoarthritis mouse model |
author |
Rotpenpian, Nattapon |
author_facet |
Rotpenpian, Nattapon Tapechum, Sompol Vattarakorn, Anchalee Chindasri, Wongsathit Care, Chit Pakaprot, Narawut Wanasuntronwong, Aree |
author_role |
author |
author2 |
Tapechum, Sompol Vattarakorn, Anchalee Chindasri, Wongsathit Care, Chit Pakaprot, Narawut Wanasuntronwong, Aree |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Rotpenpian, Nattapon Tapechum, Sompol Vattarakorn, Anchalee Chindasri, Wongsathit Care, Chit Pakaprot, Narawut Wanasuntronwong, Aree |
dc.subject.por.fl_str_mv |
Mirror-image pain Osteoarthritis Complete Freund adjuvant Temporomandibular joint Nocifensive behaviors |
topic |
Mirror-image pain Osteoarthritis Complete Freund adjuvant Temporomandibular joint Nocifensive behaviors |
description |
Mirror-image pain is a kind of pain that occurs on the contralateral side, but its pathogenesis remains unclear. Objective: To develop an osteoarthritis mouse model for investigating mirror-image pain through observing nocifensive behaviors, histological changes, and nociceptive activity at days 3, 7, 14, 21, and 28 after the chemical induction of unilateral temporomandibular joint (TMJ) osteoarthritis. Methodology: We randomly divided 6-week-old mice into sham and complete Freund adjuvant groups. To induce nocifensive behaviors, we applied 0.04 g of von Frey filament, 10 psi of air puff, and cold acetone on both sides of whisker pads at different days. The histology of TMJ on both sides was observed by hematoxylin/eosin staining and microcomputed tomography scanning. Furthermore, the nociceptive activity was evaluated using the phosphorylated cyclic AMP response element binding protein (pCREB) and a microglia marker at different days in the trigeminal subnucleus caudalis. Results: Nocifensive behaviors against mechanical and temperature stimuli on the contralateral side became stronger than the baseline on day 28, in agreement with the elevation of the pCREB and the microglia marker in the trigeminal subnucleus caudalis. Thus, hypernociception on the contralateral side occurred at day 28. Conclusions: Clearly, the TMJ model with unilateral osteoarthritis exhibited mirror-image pain. Therefore, this model is useful in investigating the pathogenesis of pain and in developing treatments. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/187119 10.1590/1678-7757-2020-0575 |
url |
https://www.revistas.usp.br/jaos/article/view/187119 |
identifier_str_mv |
10.1590/1678-7757-2020-0575 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/187119/172870 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 29 (2021); e20200575 Journal of Applied Oral Science; Vol. 29 (2021); e20200575 Journal of Applied Oral Science; v. 29 (2021); e20200575 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221681236574208 |