Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/191015 |
Resumo: | Objectives: Infection, inflammation and bone resorption are closely related events in apical periodontitis development. Therefore, we sought to investigate the role of cyclooxygenase (COX) in osteoclastogenesis and bone metabolism signaling in periapical bone tissue after bacterial lipopolysaccharide (LPS) inoculation into root canals. Methodology: Seventy two C57BL/6 mice had the root canals of the first molars inoculated with a solution containing LPS from E. coli (1.0 mg/mL) and received selective (celecoxib) or non-selective (indomethacin) COX-2 inhibitor. After 7, 14, 21 and 28 days the animals were euthanized and the tissues removed for total RNA extraction. Evaluation of gene expression was performed by qRT-PCR. Statistical analysis was performed using analysis of variance (ANOVA) followed by post-tests (α=0.05). Results: LPS induced expression of mRNA for COX-2 (Ptgs2) and PGE2 receptors (Ptger1, Ptger3 and Ptger4), indicating that cyclooxygenase is involved in periapical response to LPS. A signaling that favours bone resorption was observed because Tnfsf11 (RANKL), Vegfa, Ctsk, Mmp9, Cd36, Icam, Vcam1, Nfkb1 and Sox9 were upregulated in response to LPS. Indomethacin and celecoxib differentially modulated expression of osteoclastogenic and other bone metabolism genes: celecoxib downregulated Igf1r, Ctsk, Mmp9, Cd36, Icam1, Nfkb1, Smad3, Sox9, Csf3, Vcam1 and Itga3 whereas indomethacin inhibited Tgfbr1, Igf1r, Ctsk, Mmp9, Sox9, Cd36 and Icam1. Conclusions: We demonstrated that gene expression for COX-2 and PGE2 receptors was upregulated after LPS inoculation into the root canals. Additionally, early administration of indomethacin and celecoxib (NSAIDs) inhibited osteoclastogenic signaling. The relevance of the cyclooxygenase pathway in apical periodontitis was shown by a wide modulation in the expression of genes involved in both bone catabolism and anabolism. |
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Journal of applied oral science (Online) |
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Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockageApical periodontitisLipopolysaccharideBoneOsteoclastogenesisIndomethacinCelecoxibCyclooxygenaseObjectives: Infection, inflammation and bone resorption are closely related events in apical periodontitis development. Therefore, we sought to investigate the role of cyclooxygenase (COX) in osteoclastogenesis and bone metabolism signaling in periapical bone tissue after bacterial lipopolysaccharide (LPS) inoculation into root canals. Methodology: Seventy two C57BL/6 mice had the root canals of the first molars inoculated with a solution containing LPS from E. coli (1.0 mg/mL) and received selective (celecoxib) or non-selective (indomethacin) COX-2 inhibitor. After 7, 14, 21 and 28 days the animals were euthanized and the tissues removed for total RNA extraction. Evaluation of gene expression was performed by qRT-PCR. Statistical analysis was performed using analysis of variance (ANOVA) followed by post-tests (α=0.05). Results: LPS induced expression of mRNA for COX-2 (Ptgs2) and PGE2 receptors (Ptger1, Ptger3 and Ptger4), indicating that cyclooxygenase is involved in periapical response to LPS. A signaling that favours bone resorption was observed because Tnfsf11 (RANKL), Vegfa, Ctsk, Mmp9, Cd36, Icam, Vcam1, Nfkb1 and Sox9 were upregulated in response to LPS. Indomethacin and celecoxib differentially modulated expression of osteoclastogenic and other bone metabolism genes: celecoxib downregulated Igf1r, Ctsk, Mmp9, Cd36, Icam1, Nfkb1, Smad3, Sox9, Csf3, Vcam1 and Itga3 whereas indomethacin inhibited Tgfbr1, Igf1r, Ctsk, Mmp9, Sox9, Cd36 and Icam1. Conclusions: We demonstrated that gene expression for COX-2 and PGE2 receptors was upregulated after LPS inoculation into the root canals. Additionally, early administration of indomethacin and celecoxib (NSAIDs) inhibited osteoclastogenic signaling. The relevance of the cyclooxygenase pathway in apical periodontitis was shown by a wide modulation in the expression of genes involved in both bone catabolism and anabolism.Universidade de São Paulo. Faculdade de Odontologia de Bauru2021-09-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/19101510.1590/1678-7757-2018-0641Journal of Applied Oral Science; Vol. 27 (2019); e20180641Journal of Applied Oral Science; Vol. 27 (2019); e20180641Journal of Applied Oral Science; v. 27 (2019); e201806411678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/191015/176095Copyright (c) 2021 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRibeiro-Santos, Fernanda Regina Silva, Geyson Galo da Petean, Igor Bassi Ferreira Arnez, Maya Fernanda Manfrin Silva, Léa Assed Bezerra da Faccioli, Lúcia Helena Paula-Silva, Francisco Wanderley Garcia 2021-09-29T13:54:39Zoai:revistas.usp.br:article/191015Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2021-09-29T13:54:39Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage |
title |
Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage |
spellingShingle |
Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage Ribeiro-Santos, Fernanda Regina Apical periodontitis Lipopolysaccharide Bone Osteoclastogenesis Indomethacin Celecoxib Cyclooxygenase |
title_short |
Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage |
title_full |
Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage |
title_fullStr |
Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage |
title_full_unstemmed |
Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage |
title_sort |
Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage |
author |
Ribeiro-Santos, Fernanda Regina |
author_facet |
Ribeiro-Santos, Fernanda Regina Silva, Geyson Galo da Petean, Igor Bassi Ferreira Arnez, Maya Fernanda Manfrin Silva, Léa Assed Bezerra da Faccioli, Lúcia Helena Paula-Silva, Francisco Wanderley Garcia |
author_role |
author |
author2 |
Silva, Geyson Galo da Petean, Igor Bassi Ferreira Arnez, Maya Fernanda Manfrin Silva, Léa Assed Bezerra da Faccioli, Lúcia Helena Paula-Silva, Francisco Wanderley Garcia |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Ribeiro-Santos, Fernanda Regina Silva, Geyson Galo da Petean, Igor Bassi Ferreira Arnez, Maya Fernanda Manfrin Silva, Léa Assed Bezerra da Faccioli, Lúcia Helena Paula-Silva, Francisco Wanderley Garcia |
dc.subject.por.fl_str_mv |
Apical periodontitis Lipopolysaccharide Bone Osteoclastogenesis Indomethacin Celecoxib Cyclooxygenase |
topic |
Apical periodontitis Lipopolysaccharide Bone Osteoclastogenesis Indomethacin Celecoxib Cyclooxygenase |
description |
Objectives: Infection, inflammation and bone resorption are closely related events in apical periodontitis development. Therefore, we sought to investigate the role of cyclooxygenase (COX) in osteoclastogenesis and bone metabolism signaling in periapical bone tissue after bacterial lipopolysaccharide (LPS) inoculation into root canals. Methodology: Seventy two C57BL/6 mice had the root canals of the first molars inoculated with a solution containing LPS from E. coli (1.0 mg/mL) and received selective (celecoxib) or non-selective (indomethacin) COX-2 inhibitor. After 7, 14, 21 and 28 days the animals were euthanized and the tissues removed for total RNA extraction. Evaluation of gene expression was performed by qRT-PCR. Statistical analysis was performed using analysis of variance (ANOVA) followed by post-tests (α=0.05). Results: LPS induced expression of mRNA for COX-2 (Ptgs2) and PGE2 receptors (Ptger1, Ptger3 and Ptger4), indicating that cyclooxygenase is involved in periapical response to LPS. A signaling that favours bone resorption was observed because Tnfsf11 (RANKL), Vegfa, Ctsk, Mmp9, Cd36, Icam, Vcam1, Nfkb1 and Sox9 were upregulated in response to LPS. Indomethacin and celecoxib differentially modulated expression of osteoclastogenic and other bone metabolism genes: celecoxib downregulated Igf1r, Ctsk, Mmp9, Cd36, Icam1, Nfkb1, Smad3, Sox9, Csf3, Vcam1 and Itga3 whereas indomethacin inhibited Tgfbr1, Igf1r, Ctsk, Mmp9, Sox9, Cd36 and Icam1. Conclusions: We demonstrated that gene expression for COX-2 and PGE2 receptors was upregulated after LPS inoculation into the root canals. Additionally, early administration of indomethacin and celecoxib (NSAIDs) inhibited osteoclastogenic signaling. The relevance of the cyclooxygenase pathway in apical periodontitis was shown by a wide modulation in the expression of genes involved in both bone catabolism and anabolism. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/191015 10.1590/1678-7757-2018-0641 |
url |
https://www.revistas.usp.br/jaos/article/view/191015 |
identifier_str_mv |
10.1590/1678-7757-2018-0641 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/191015/176095 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 27 (2019); e20180641 Journal of Applied Oral Science; Vol. 27 (2019); e20180641 Journal of Applied Oral Science; v. 27 (2019); e20180641 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221682494865408 |