Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage

Detalhes bibliográficos
Autor(a) principal: Ribeiro-Santos, Fernanda Regina
Data de Publicação: 2021
Outros Autores: Silva, Geyson Galo da, Petean, Igor Bassi Ferreira, Arnez, Maya Fernanda Manfrin, Silva, Léa Assed Bezerra da, Faccioli, Lúcia Helena, Paula-Silva, Francisco Wanderley Garcia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/191015
Resumo: Objectives: Infection, inflammation and bone resorption are closely related events in apical periodontitis development. Therefore, we sought to investigate the role of cyclooxygenase (COX) in osteoclastogenesis and bone metabolism signaling in periapical bone tissue after bacterial lipopolysaccharide (LPS) inoculation into root canals. Methodology: Seventy two C57BL/6 mice had the root canals of the first molars inoculated with a solution containing LPS from E. coli (1.0 mg/mL) and received selective (celecoxib) or non-selective (indomethacin) COX-2 inhibitor. After 7, 14, 21 and 28 days the animals were euthanized and the tissues removed for total RNA extraction. Evaluation of gene expression was performed by qRT-PCR. Statistical analysis was performed using analysis of variance (ANOVA) followed by post-tests (α=0.05). Results: LPS induced expression of mRNA for COX-2 (Ptgs2) and PGE2 receptors (Ptger1, Ptger3 and Ptger4), indicating that cyclooxygenase is involved in periapical response to LPS. A signaling that favours bone resorption was observed because Tnfsf11 (RANKL), Vegfa, Ctsk, Mmp9, Cd36, Icam, Vcam1, Nfkb1 and Sox9 were upregulated in response to LPS. Indomethacin and celecoxib differentially modulated expression of osteoclastogenic and other bone metabolism genes: celecoxib downregulated Igf1r, Ctsk, Mmp9, Cd36, Icam1, Nfkb1, Smad3, Sox9, Csf3, Vcam1 and Itga3 whereas indomethacin inhibited Tgfbr1, Igf1r, Ctsk, Mmp9, Sox9, Cd36 and Icam1. Conclusions: We demonstrated that gene expression for COX-2 and PGE2 receptors was upregulated after LPS inoculation into the root canals. Additionally, early administration of indomethacin and celecoxib (NSAIDs) inhibited osteoclastogenic signaling. The relevance of the cyclooxygenase pathway in apical periodontitis was shown by a wide modulation in the expression of genes involved in both bone catabolism and anabolism.
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spelling Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockageApical periodontitisLipopolysaccharideBoneOsteoclastogenesisIndomethacinCelecoxibCyclooxygenaseObjectives: Infection, inflammation and bone resorption are closely related events in apical periodontitis development. Therefore, we sought to investigate the role of cyclooxygenase (COX) in osteoclastogenesis and bone metabolism signaling in periapical bone tissue after bacterial lipopolysaccharide (LPS) inoculation into root canals. Methodology: Seventy two C57BL/6 mice had the root canals of the first molars inoculated with a solution containing LPS from E. coli (1.0 mg/mL) and received selective (celecoxib) or non-selective (indomethacin) COX-2 inhibitor. After 7, 14, 21 and 28 days the animals were euthanized and the tissues removed for total RNA extraction. Evaluation of gene expression was performed by qRT-PCR. Statistical analysis was performed using analysis of variance (ANOVA) followed by post-tests (α=0.05). Results: LPS induced expression of mRNA for COX-2 (Ptgs2) and PGE2 receptors (Ptger1, Ptger3 and Ptger4), indicating that cyclooxygenase is involved in periapical response to LPS. A signaling that favours bone resorption was observed because Tnfsf11 (RANKL), Vegfa, Ctsk, Mmp9, Cd36, Icam, Vcam1, Nfkb1 and Sox9 were upregulated in response to LPS. Indomethacin and celecoxib differentially modulated expression of osteoclastogenic and other bone metabolism genes: celecoxib downregulated Igf1r, Ctsk, Mmp9, Cd36, Icam1, Nfkb1, Smad3, Sox9, Csf3, Vcam1 and Itga3 whereas indomethacin inhibited Tgfbr1, Igf1r, Ctsk, Mmp9, Sox9, Cd36 and Icam1. Conclusions: We demonstrated that gene expression for COX-2 and PGE2 receptors was upregulated after LPS inoculation into the root canals. Additionally, early administration of indomethacin and celecoxib (NSAIDs) inhibited osteoclastogenic signaling. The relevance of the cyclooxygenase pathway in apical periodontitis was shown by a wide modulation in the expression of genes involved in both bone catabolism and anabolism.Universidade de São Paulo. Faculdade de Odontologia de Bauru2021-09-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/19101510.1590/1678-7757-2018-0641Journal of Applied Oral Science; Vol. 27 (2019); e20180641Journal of Applied Oral Science; Vol. 27 (2019); e20180641Journal of Applied Oral Science; v. 27 (2019); e201806411678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/191015/176095Copyright (c) 2021 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRibeiro-Santos, Fernanda Regina Silva, Geyson Galo da Petean, Igor Bassi Ferreira Arnez, Maya Fernanda Manfrin Silva, Léa Assed Bezerra da Faccioli, Lúcia Helena Paula-Silva, Francisco Wanderley Garcia 2021-09-29T13:54:39Zoai:revistas.usp.br:article/191015Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2021-09-29T13:54:39Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage
title Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage
spellingShingle Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage
Ribeiro-Santos, Fernanda Regina
Apical periodontitis
Lipopolysaccharide
Bone
Osteoclastogenesis
Indomethacin
Celecoxib
Cyclooxygenase
title_short Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage
title_full Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage
title_fullStr Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage
title_full_unstemmed Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage
title_sort Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage
author Ribeiro-Santos, Fernanda Regina
author_facet Ribeiro-Santos, Fernanda Regina
Silva, Geyson Galo da
Petean, Igor Bassi Ferreira
Arnez, Maya Fernanda Manfrin
Silva, Léa Assed Bezerra da
Faccioli, Lúcia Helena
Paula-Silva, Francisco Wanderley Garcia
author_role author
author2 Silva, Geyson Galo da
Petean, Igor Bassi Ferreira
Arnez, Maya Fernanda Manfrin
Silva, Léa Assed Bezerra da
Faccioli, Lúcia Helena
Paula-Silva, Francisco Wanderley Garcia
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ribeiro-Santos, Fernanda Regina
Silva, Geyson Galo da
Petean, Igor Bassi Ferreira
Arnez, Maya Fernanda Manfrin
Silva, Léa Assed Bezerra da
Faccioli, Lúcia Helena
Paula-Silva, Francisco Wanderley Garcia
dc.subject.por.fl_str_mv Apical periodontitis
Lipopolysaccharide
Bone
Osteoclastogenesis
Indomethacin
Celecoxib
Cyclooxygenase
topic Apical periodontitis
Lipopolysaccharide
Bone
Osteoclastogenesis
Indomethacin
Celecoxib
Cyclooxygenase
description Objectives: Infection, inflammation and bone resorption are closely related events in apical periodontitis development. Therefore, we sought to investigate the role of cyclooxygenase (COX) in osteoclastogenesis and bone metabolism signaling in periapical bone tissue after bacterial lipopolysaccharide (LPS) inoculation into root canals. Methodology: Seventy two C57BL/6 mice had the root canals of the first molars inoculated with a solution containing LPS from E. coli (1.0 mg/mL) and received selective (celecoxib) or non-selective (indomethacin) COX-2 inhibitor. After 7, 14, 21 and 28 days the animals were euthanized and the tissues removed for total RNA extraction. Evaluation of gene expression was performed by qRT-PCR. Statistical analysis was performed using analysis of variance (ANOVA) followed by post-tests (α=0.05). Results: LPS induced expression of mRNA for COX-2 (Ptgs2) and PGE2 receptors (Ptger1, Ptger3 and Ptger4), indicating that cyclooxygenase is involved in periapical response to LPS. A signaling that favours bone resorption was observed because Tnfsf11 (RANKL), Vegfa, Ctsk, Mmp9, Cd36, Icam, Vcam1, Nfkb1 and Sox9 were upregulated in response to LPS. Indomethacin and celecoxib differentially modulated expression of osteoclastogenic and other bone metabolism genes: celecoxib downregulated Igf1r, Ctsk, Mmp9, Cd36, Icam1, Nfkb1, Smad3, Sox9, Csf3, Vcam1 and Itga3 whereas indomethacin inhibited Tgfbr1, Igf1r, Ctsk, Mmp9, Sox9, Cd36 and Icam1. Conclusions: We demonstrated that gene expression for COX-2 and PGE2 receptors was upregulated after LPS inoculation into the root canals. Additionally, early administration of indomethacin and celecoxib (NSAIDs) inhibited osteoclastogenic signaling. The relevance of the cyclooxygenase pathway in apical periodontitis was shown by a wide modulation in the expression of genes involved in both bone catabolism and anabolism.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-29
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/191015
10.1590/1678-7757-2018-0641
url https://www.revistas.usp.br/jaos/article/view/191015
identifier_str_mv 10.1590/1678-7757-2018-0641
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/191015/176095
dc.rights.driver.fl_str_mv Copyright (c) 2021 Journal of Applied Oral Science
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Journal of Applied Oral Science
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 27 (2019); e20180641
Journal of Applied Oral Science; Vol. 27 (2019); e20180641
Journal of Applied Oral Science; v. 27 (2019); e20180641
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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