miR-9-1 gene methylation and DNMT3B (rs2424913) polymorphism may contribute to periodontitis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572020000100433 |
Resumo: | Abstract Genetic and epigenetic changes have been associated with periodontitis in various genes; however, little is known about genes involved in epigenetic mechanisms and in oxidative stress. Objective: This study aims to investigate the association of polymorphisms C677T in MTHFR (rs1801133) and −149C→T in DNMT3B (rs2424913), as well as the methylation profiles of MTHFR, miR-9-1, miR-9-3, SOD1, and CAT with periodontitis. The association between polymorphisms and DNA methylation profiles was also analyzed. Methodology: The population studied was composed of 100 nonsmokers of both sexes, divided into healthy and periodontitis groups. Genomic DNA was extracted from the epithelial buccal cells, which were collected through a mouthwash. Polymorphism analysis was performed through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), while methylation-specific PCR (MSP) or combined bisulfite restriction analysis techniques were applied for methylation analysis. Results: For DNMT3B, the T allele and the TT genotype were detected more frequently in the periodontitis group, as well as the methylated profile on the miR-9-1 promoter region. There was also a tendency towards promoter region methylation on the CAT sequence of individuals with periodontal disease. Conclusion: The polymorphism −149C→T in DNMT3B (rs2424913) and the methylated profile of the miR-9-1 promoter region are associated with periodontitis. |
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USP-17 |
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Journal of applied oral science (Online) |
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miR-9-1 gene methylation and DNMT3B (rs2424913) polymorphism may contribute to periodontitisPeriodontitisEpigeneticDNA methylationPolymorphismOxidative stressMicroRNAAbstract Genetic and epigenetic changes have been associated with periodontitis in various genes; however, little is known about genes involved in epigenetic mechanisms and in oxidative stress. Objective: This study aims to investigate the association of polymorphisms C677T in MTHFR (rs1801133) and −149C→T in DNMT3B (rs2424913), as well as the methylation profiles of MTHFR, miR-9-1, miR-9-3, SOD1, and CAT with periodontitis. The association between polymorphisms and DNA methylation profiles was also analyzed. Methodology: The population studied was composed of 100 nonsmokers of both sexes, divided into healthy and periodontitis groups. Genomic DNA was extracted from the epithelial buccal cells, which were collected through a mouthwash. Polymorphism analysis was performed through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), while methylation-specific PCR (MSP) or combined bisulfite restriction analysis techniques were applied for methylation analysis. Results: For DNMT3B, the T allele and the TT genotype were detected more frequently in the periodontitis group, as well as the methylated profile on the miR-9-1 promoter region. There was also a tendency towards promoter region methylation on the CAT sequence of individuals with periodontal disease. Conclusion: The polymorphism −149C→T in DNMT3B (rs2424913) and the methylated profile of the miR-9-1 promoter region are associated with periodontitis.Faculdade De Odontologia De Bauru - USP2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572020000100433Journal of Applied Oral Science v.28 2020reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-7757-2019-0583info:eu-repo/semantics/openAccessCoêlho,Marina CastroQueiroz,Ingrid CostaViana Filho,José Maria ChagasAquino,Sabrina Garcia dePersuhn,Darlene CamatiOliveira,Naila Francis Paulo deeng2020-03-30T00:00:00Zoai:scielo:S1678-77572020000100433Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2020-03-30T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
miR-9-1 gene methylation and DNMT3B (rs2424913) polymorphism may contribute to periodontitis |
title |
miR-9-1 gene methylation and DNMT3B (rs2424913) polymorphism may contribute to periodontitis |
spellingShingle |
miR-9-1 gene methylation and DNMT3B (rs2424913) polymorphism may contribute to periodontitis Coêlho,Marina Castro Periodontitis Epigenetic DNA methylation Polymorphism Oxidative stress MicroRNA |
title_short |
miR-9-1 gene methylation and DNMT3B (rs2424913) polymorphism may contribute to periodontitis |
title_full |
miR-9-1 gene methylation and DNMT3B (rs2424913) polymorphism may contribute to periodontitis |
title_fullStr |
miR-9-1 gene methylation and DNMT3B (rs2424913) polymorphism may contribute to periodontitis |
title_full_unstemmed |
miR-9-1 gene methylation and DNMT3B (rs2424913) polymorphism may contribute to periodontitis |
title_sort |
miR-9-1 gene methylation and DNMT3B (rs2424913) polymorphism may contribute to periodontitis |
author |
Coêlho,Marina Castro |
author_facet |
Coêlho,Marina Castro Queiroz,Ingrid Costa Viana Filho,José Maria Chagas Aquino,Sabrina Garcia de Persuhn,Darlene Camati Oliveira,Naila Francis Paulo de |
author_role |
author |
author2 |
Queiroz,Ingrid Costa Viana Filho,José Maria Chagas Aquino,Sabrina Garcia de Persuhn,Darlene Camati Oliveira,Naila Francis Paulo de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Coêlho,Marina Castro Queiroz,Ingrid Costa Viana Filho,José Maria Chagas Aquino,Sabrina Garcia de Persuhn,Darlene Camati Oliveira,Naila Francis Paulo de |
dc.subject.por.fl_str_mv |
Periodontitis Epigenetic DNA methylation Polymorphism Oxidative stress MicroRNA |
topic |
Periodontitis Epigenetic DNA methylation Polymorphism Oxidative stress MicroRNA |
description |
Abstract Genetic and epigenetic changes have been associated with periodontitis in various genes; however, little is known about genes involved in epigenetic mechanisms and in oxidative stress. Objective: This study aims to investigate the association of polymorphisms C677T in MTHFR (rs1801133) and −149C→T in DNMT3B (rs2424913), as well as the methylation profiles of MTHFR, miR-9-1, miR-9-3, SOD1, and CAT with periodontitis. The association between polymorphisms and DNA methylation profiles was also analyzed. Methodology: The population studied was composed of 100 nonsmokers of both sexes, divided into healthy and periodontitis groups. Genomic DNA was extracted from the epithelial buccal cells, which were collected through a mouthwash. Polymorphism analysis was performed through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), while methylation-specific PCR (MSP) or combined bisulfite restriction analysis techniques were applied for methylation analysis. Results: For DNMT3B, the T allele and the TT genotype were detected more frequently in the periodontitis group, as well as the methylated profile on the miR-9-1 promoter region. There was also a tendency towards promoter region methylation on the CAT sequence of individuals with periodontal disease. Conclusion: The polymorphism −149C→T in DNMT3B (rs2424913) and the methylated profile of the miR-9-1 promoter region are associated with periodontitis. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572020000100433 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572020000100433 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-7757-2019-0583 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science v.28 2020 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1748936440512774144 |