Cupriavidus necator strains: zinc and cadmium tolerance and bioaccumulation

Detalhes bibliográficos
Autor(a) principal: Ferreira, Paulo Ademar Avelar
Data de Publicação: 2018
Outros Autores: Bomfeti, Cleide Aparecida, Soares, Cláudio Roberto Fonsêca de Souza, Soares, Bruno Lima, Moreira, Fatima Maria de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: https://www.revistas.usp.br/sa/article/view/147484
Resumo: Among soil microorganisms, the genus Cupriavidus has garnered particular scientific, economic and ecological interest because of its ability to fix nitrogen and tolerate high concentrations of metals. The aim of this study was to analyze four strains of Cupriavidus necator for their ability to tolerate and bioaccumulate cadmium and zinc. The tolerance of these strains to these metals was assessed in liquid culture medium containing different concentrations of Zn + Cd and in soil solutions and soils contaminated with multiple elements including Zn, Cd, Cu and Pb. The four strains showed high tolerance to Zn and Cd, both in culture medium and when inoculated into contaminated soil solutions or multi-element contaminated soil. The UFLA02-71 strain displayed the highest ability to bioaccumulate these metals. It was able to accumulate 93.76 µmol g cell−1 of Zn and 16.03 µmol g cell−1 of Cd when cultured in liquid medium with a total heavy metal concentration of 9,140 µmol L−1 (9,000 Zn + 140 Cd) and was able to accumulate 16.98 µmol g cell−1 of Cd in the soil solution. An increase in the pH of the culture medium resulting from the growth of the C. necator strains reduced the Zn2+ and Cd2+ ions in the medium and increased the concentrations of the ZnHPO4 and CdHPO4 species in solution. Thus, we concluded that they show great potential for use in the bioremediation of HM-contaminated areas.
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spelling Cupriavidus necator strains: zinc and cadmium tolerance and bioaccumulationβ-proteobacteriabioremediationheavy-metalsoil pollution Among soil microorganisms, the genus Cupriavidus has garnered particular scientific, economic and ecological interest because of its ability to fix nitrogen and tolerate high concentrations of metals. The aim of this study was to analyze four strains of Cupriavidus necator for their ability to tolerate and bioaccumulate cadmium and zinc. The tolerance of these strains to these metals was assessed in liquid culture medium containing different concentrations of Zn + Cd and in soil solutions and soils contaminated with multiple elements including Zn, Cd, Cu and Pb. The four strains showed high tolerance to Zn and Cd, both in culture medium and when inoculated into contaminated soil solutions or multi-element contaminated soil. The UFLA02-71 strain displayed the highest ability to bioaccumulate these metals. It was able to accumulate 93.76 µmol g cell−1 of Zn and 16.03 µmol g cell−1 of Cd when cultured in liquid medium with a total heavy metal concentration of 9,140 µmol L−1 (9,000 Zn + 140 Cd) and was able to accumulate 16.98 µmol g cell−1 of Cd in the soil solution. An increase in the pH of the culture medium resulting from the growth of the C. necator strains reduced the Zn2+ and Cd2+ ions in the medium and increased the concentrations of the ZnHPO4 and CdHPO4 species in solution. Thus, we concluded that they show great potential for use in the bioremediation of HM-contaminated areas.Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz2018-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/sa/article/view/14748410.1590/1678-992x-2017-0071Scientia Agricola; v. 75 n. 6 (2018); 452-460Scientia Agricola; Vol. 75 Núm. 6 (2018); 452-460Scientia Agricola; Vol. 75 No. 6 (2018); 452-4601678-992X0103-9016reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/sa/article/view/147484/141000Copyright (c) 2018 Scientia Agricolainfo:eu-repo/semantics/openAccessFerreira, Paulo Ademar AvelarBomfeti, Cleide AparecidaSoares, Cláudio Roberto Fonsêca de SouzaSoares, Bruno LimaMoreira, Fatima Maria de Souza2018-06-25T17:28:21Zoai:revistas.usp.br:article/147484Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2018-06-25T17:28:21Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Cupriavidus necator strains: zinc and cadmium tolerance and bioaccumulation
title Cupriavidus necator strains: zinc and cadmium tolerance and bioaccumulation
spellingShingle Cupriavidus necator strains: zinc and cadmium tolerance and bioaccumulation
Ferreira, Paulo Ademar Avelar
β-proteobacteria
bioremediation
heavy-metal
soil pollution
title_short Cupriavidus necator strains: zinc and cadmium tolerance and bioaccumulation
title_full Cupriavidus necator strains: zinc and cadmium tolerance and bioaccumulation
title_fullStr Cupriavidus necator strains: zinc and cadmium tolerance and bioaccumulation
title_full_unstemmed Cupriavidus necator strains: zinc and cadmium tolerance and bioaccumulation
title_sort Cupriavidus necator strains: zinc and cadmium tolerance and bioaccumulation
author Ferreira, Paulo Ademar Avelar
author_facet Ferreira, Paulo Ademar Avelar
Bomfeti, Cleide Aparecida
Soares, Cláudio Roberto Fonsêca de Souza
Soares, Bruno Lima
Moreira, Fatima Maria de Souza
author_role author
author2 Bomfeti, Cleide Aparecida
Soares, Cláudio Roberto Fonsêca de Souza
Soares, Bruno Lima
Moreira, Fatima Maria de Souza
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ferreira, Paulo Ademar Avelar
Bomfeti, Cleide Aparecida
Soares, Cláudio Roberto Fonsêca de Souza
Soares, Bruno Lima
Moreira, Fatima Maria de Souza
dc.subject.por.fl_str_mv β-proteobacteria
bioremediation
heavy-metal
soil pollution
topic β-proteobacteria
bioremediation
heavy-metal
soil pollution
description Among soil microorganisms, the genus Cupriavidus has garnered particular scientific, economic and ecological interest because of its ability to fix nitrogen and tolerate high concentrations of metals. The aim of this study was to analyze four strains of Cupriavidus necator for their ability to tolerate and bioaccumulate cadmium and zinc. The tolerance of these strains to these metals was assessed in liquid culture medium containing different concentrations of Zn + Cd and in soil solutions and soils contaminated with multiple elements including Zn, Cd, Cu and Pb. The four strains showed high tolerance to Zn and Cd, both in culture medium and when inoculated into contaminated soil solutions or multi-element contaminated soil. The UFLA02-71 strain displayed the highest ability to bioaccumulate these metals. It was able to accumulate 93.76 µmol g cell−1 of Zn and 16.03 µmol g cell−1 of Cd when cultured in liquid medium with a total heavy metal concentration of 9,140 µmol L−1 (9,000 Zn + 140 Cd) and was able to accumulate 16.98 µmol g cell−1 of Cd in the soil solution. An increase in the pH of the culture medium resulting from the growth of the C. necator strains reduced the Zn2+ and Cd2+ ions in the medium and increased the concentrations of the ZnHPO4 and CdHPO4 species in solution. Thus, we concluded that they show great potential for use in the bioremediation of HM-contaminated areas.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/sa/article/view/147484
10.1590/1678-992x-2017-0071
url https://www.revistas.usp.br/sa/article/view/147484
identifier_str_mv 10.1590/1678-992x-2017-0071
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/sa/article/view/147484/141000
dc.rights.driver.fl_str_mv Copyright (c) 2018 Scientia Agricola
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Scientia Agricola
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
publisher.none.fl_str_mv Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
dc.source.none.fl_str_mv Scientia Agricola; v. 75 n. 6 (2018); 452-460
Scientia Agricola; Vol. 75 Núm. 6 (2018); 452-460
Scientia Agricola; Vol. 75 No. 6 (2018); 452-460
1678-992X
0103-9016
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
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