Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs

Detalhes bibliográficos
Autor(a) principal: Paim, Tiago do Prado
Data de Publicação: 2019
Outros Autores: Viana, Pauline, Tilburg, Mauricio Fraga van, Moura, Arlindo de Alencar, Souza, Jurandir Rodrigues de, McManus, Concepta, Abdalla, Adibe Luiz, Louvandini, Helder
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: https://www.revistas.usp.br/sa/article/view/160634
Resumo: Gossypol easily pairs with lysine side chains and enzymes involved in the cellular growth process. The effect of gossypol (a compound present in cotton co-products) in ruminant metabolism and meat quality is not yet clear. This study was undertaken in order to evaluate the effects of cotton co-products in lamb muscle proteome. Twenty-four Santa Inês ram lambs, 5-months old (20.6 ± 1.9 kg), were randomly assigned to four treatments: control (without cottonseed), whole cottonseed, cottonseed meal and high oil cottonseed meal. At 95 days into the experiment, lambs were slaughtered and samples from Longissimus dorsi were collected. Proteins were extracted and analyzed by 2-D electrophoresis. Spots showing a significant effect from the treatment (the “treatment effect”) and present in more than 90 % of the samples were identified using mass spectrometry. Cotton co-products decreased the abundance of aldehyde and malate dehydrogenases, creatine kinase M-type and Adenosine triphosphate (ATP) synthase. They also increased four proteins related to muscle contraction. Thus, feeding cotton co-products to lambs changed the abundance of important muscle proteins. A cotton co-product diet induced a negative impact on the energy supply of muscle cells and, consequently, the abundance of ATP dependent proteins (contractile apparatus) increased, probably in order to offset and maintain muscle function. These proteomic changes can promote our understanding of alterations in the sensorial properties of meat due to cotton co-product diets in further investigations.
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spelling Feeding effects of cottonseed and its co-products on the meat proteome from ram lambsgossypoldehydrogenaseskeletal musclesheepfeedlotGossypol easily pairs with lysine side chains and enzymes involved in the cellular growth process. The effect of gossypol (a compound present in cotton co-products) in ruminant metabolism and meat quality is not yet clear. This study was undertaken in order to evaluate the effects of cotton co-products in lamb muscle proteome. Twenty-four Santa Inês ram lambs, 5-months old (20.6 ± 1.9 kg), were randomly assigned to four treatments: control (without cottonseed), whole cottonseed, cottonseed meal and high oil cottonseed meal. At 95 days into the experiment, lambs were slaughtered and samples from Longissimus dorsi were collected. Proteins were extracted and analyzed by 2-D electrophoresis. Spots showing a significant effect from the treatment (the “treatment effect”) and present in more than 90 % of the samples were identified using mass spectrometry. Cotton co-products decreased the abundance of aldehyde and malate dehydrogenases, creatine kinase M-type and Adenosine triphosphate (ATP) synthase. They also increased four proteins related to muscle contraction. Thus, feeding cotton co-products to lambs changed the abundance of important muscle proteins. A cotton co-product diet induced a negative impact on the energy supply of muscle cells and, consequently, the abundance of ATP dependent proteins (contractile apparatus) increased, probably in order to offset and maintain muscle function. These proteomic changes can promote our understanding of alterations in the sensorial properties of meat due to cotton co-product diets in further investigations.Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz2019-08-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/sa/article/view/16063410.1590/1678-992x-2018-0072Scientia Agricola; v. 76 n. 6 (2019); 463-472Scientia Agricola; Vol. 76 Núm. 6 (2019); 463-472Scientia Agricola; Vol. 76 No. 6 (2019); 463-4721678-992X0103-9016reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/sa/article/view/160634/154888Copyright (c) 2019 Scientia Agricolainfo:eu-repo/semantics/openAccessPaim, Tiago do PradoViana, PaulineTilburg, Mauricio Fraga vanMoura, Arlindo de AlencarSouza, Jurandir Rodrigues deMcManus, ConceptaAbdalla, Adibe LuizLouvandini, Helder2019-08-02T17:12:54Zoai:revistas.usp.br:article/160634Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2019-08-02T17:12:54Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs
title Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs
spellingShingle Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs
Paim, Tiago do Prado
gossypol
dehydrogenase
skeletal muscle
sheep
feedlot
title_short Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs
title_full Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs
title_fullStr Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs
title_full_unstemmed Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs
title_sort Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs
author Paim, Tiago do Prado
author_facet Paim, Tiago do Prado
Viana, Pauline
Tilburg, Mauricio Fraga van
Moura, Arlindo de Alencar
Souza, Jurandir Rodrigues de
McManus, Concepta
Abdalla, Adibe Luiz
Louvandini, Helder
author_role author
author2 Viana, Pauline
Tilburg, Mauricio Fraga van
Moura, Arlindo de Alencar
Souza, Jurandir Rodrigues de
McManus, Concepta
Abdalla, Adibe Luiz
Louvandini, Helder
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Paim, Tiago do Prado
Viana, Pauline
Tilburg, Mauricio Fraga van
Moura, Arlindo de Alencar
Souza, Jurandir Rodrigues de
McManus, Concepta
Abdalla, Adibe Luiz
Louvandini, Helder
dc.subject.por.fl_str_mv gossypol
dehydrogenase
skeletal muscle
sheep
feedlot
topic gossypol
dehydrogenase
skeletal muscle
sheep
feedlot
description Gossypol easily pairs with lysine side chains and enzymes involved in the cellular growth process. The effect of gossypol (a compound present in cotton co-products) in ruminant metabolism and meat quality is not yet clear. This study was undertaken in order to evaluate the effects of cotton co-products in lamb muscle proteome. Twenty-four Santa Inês ram lambs, 5-months old (20.6 ± 1.9 kg), were randomly assigned to four treatments: control (without cottonseed), whole cottonseed, cottonseed meal and high oil cottonseed meal. At 95 days into the experiment, lambs were slaughtered and samples from Longissimus dorsi were collected. Proteins were extracted and analyzed by 2-D electrophoresis. Spots showing a significant effect from the treatment (the “treatment effect”) and present in more than 90 % of the samples were identified using mass spectrometry. Cotton co-products decreased the abundance of aldehyde and malate dehydrogenases, creatine kinase M-type and Adenosine triphosphate (ATP) synthase. They also increased four proteins related to muscle contraction. Thus, feeding cotton co-products to lambs changed the abundance of important muscle proteins. A cotton co-product diet induced a negative impact on the energy supply of muscle cells and, consequently, the abundance of ATP dependent proteins (contractile apparatus) increased, probably in order to offset and maintain muscle function. These proteomic changes can promote our understanding of alterations in the sensorial properties of meat due to cotton co-product diets in further investigations.
publishDate 2019
dc.date.none.fl_str_mv 2019-08-02
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/sa/article/view/160634
10.1590/1678-992x-2018-0072
url https://www.revistas.usp.br/sa/article/view/160634
identifier_str_mv 10.1590/1678-992x-2018-0072
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/sa/article/view/160634/154888
dc.rights.driver.fl_str_mv Copyright (c) 2019 Scientia Agricola
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Scientia Agricola
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
publisher.none.fl_str_mv Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
dc.source.none.fl_str_mv Scientia Agricola; v. 76 n. 6 (2019); 463-472
Scientia Agricola; Vol. 76 Núm. 6 (2019); 463-472
Scientia Agricola; Vol. 76 No. 6 (2019); 463-472
1678-992X
0103-9016
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
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