Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500011 |
Resumo: | In commercial plantations of soybean in both the Southern and the Cerrado regions, contributions from biological nitrogen fixation (BNF) are generally proportionately high. When using the 15N natural abundance technique to quantify BNF inputs, it is essential to determine, with accuracy, the 15N abundance of the N derived from BNF (the 'B' value). This study aimed to determine the effect of four recommended strains of Bradyrhizobium spp. (two B. japonicum and two B. elkanii) on the 'B' value of soybean grown in pots in an open field using an equation based on the determination of δ15N natural abundance in a non-labelled soil, and estimate of the contribution of BNF derived from the use of 15N-isotope dilution in soils enriched with 15N. To evaluate N2 fixation by soybean, three non-N2-fixing reference crops were grown under the same conditions. Regardless of Bradyrhizobium strain, no differences were observed in dry matter, nodule weight and total N between labelled and non-labelled soil. The N2 fixation of the soybeans grown in the two soil conditions were similar. The mean 'B' values of the soybeans inoculated with the B. japonicum strains were -1.84 and -0.50 , while those inoculated with B. elkanii were -3.67 and -1.0 , for the shoot tissue and the whole plant, respectively. Finally, the 'B' value for the soybean crop varied considerably in function of the inoculated Bradyrhizobium strain, being most important when only the shoot tissue was utilised to estimate the proportion of N in the plant derived from N2 fixation. |
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Scientia Agrícola (Online) |
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Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybeanBradyrhizobium elkaniiBradyrhizobium japonicumGlycine max'B' valueIn commercial plantations of soybean in both the Southern and the Cerrado regions, contributions from biological nitrogen fixation (BNF) are generally proportionately high. When using the 15N natural abundance technique to quantify BNF inputs, it is essential to determine, with accuracy, the 15N abundance of the N derived from BNF (the 'B' value). This study aimed to determine the effect of four recommended strains of Bradyrhizobium spp. (two B. japonicum and two B. elkanii) on the 'B' value of soybean grown in pots in an open field using an equation based on the determination of δ15N natural abundance in a non-labelled soil, and estimate of the contribution of BNF derived from the use of 15N-isotope dilution in soils enriched with 15N. To evaluate N2 fixation by soybean, three non-N2-fixing reference crops were grown under the same conditions. Regardless of Bradyrhizobium strain, no differences were observed in dry matter, nodule weight and total N between labelled and non-labelled soil. The N2 fixation of the soybeans grown in the two soil conditions were similar. The mean 'B' values of the soybeans inoculated with the B. japonicum strains were -1.84 and -0.50 , while those inoculated with B. elkanii were -3.67 and -1.0 , for the shoot tissue and the whole plant, respectively. Finally, the 'B' value for the soybean crop varied considerably in function of the inoculated Bradyrhizobium strain, being most important when only the shoot tissue was utilised to estimate the proportion of N in the plant derived from N2 fixation.Escola Superior de Agricultura "Luiz de Queiroz"2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500011Scientia Agricola v.65 n.5 2008reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162008000500011info:eu-repo/semantics/openAccessGuimarães,Ana PaulaMorais,Rafael Fiusa deUrquiaga,SegundoBoddey,Robert MichaelAlves,Bruno José Rodrigueseng2008-09-16T00:00:00Zoai:scielo:S0103-90162008000500011Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2008-09-16T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean |
title |
Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean |
spellingShingle |
Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean Guimarães,Ana Paula Bradyrhizobium elkanii Bradyrhizobium japonicum Glycine max 'B' value |
title_short |
Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean |
title_full |
Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean |
title_fullStr |
Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean |
title_full_unstemmed |
Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean |
title_sort |
Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean |
author |
Guimarães,Ana Paula |
author_facet |
Guimarães,Ana Paula Morais,Rafael Fiusa de Urquiaga,Segundo Boddey,Robert Michael Alves,Bruno José Rodrigues |
author_role |
author |
author2 |
Morais,Rafael Fiusa de Urquiaga,Segundo Boddey,Robert Michael Alves,Bruno José Rodrigues |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Guimarães,Ana Paula Morais,Rafael Fiusa de Urquiaga,Segundo Boddey,Robert Michael Alves,Bruno José Rodrigues |
dc.subject.por.fl_str_mv |
Bradyrhizobium elkanii Bradyrhizobium japonicum Glycine max 'B' value |
topic |
Bradyrhizobium elkanii Bradyrhizobium japonicum Glycine max 'B' value |
description |
In commercial plantations of soybean in both the Southern and the Cerrado regions, contributions from biological nitrogen fixation (BNF) are generally proportionately high. When using the 15N natural abundance technique to quantify BNF inputs, it is essential to determine, with accuracy, the 15N abundance of the N derived from BNF (the 'B' value). This study aimed to determine the effect of four recommended strains of Bradyrhizobium spp. (two B. japonicum and two B. elkanii) on the 'B' value of soybean grown in pots in an open field using an equation based on the determination of δ15N natural abundance in a non-labelled soil, and estimate of the contribution of BNF derived from the use of 15N-isotope dilution in soils enriched with 15N. To evaluate N2 fixation by soybean, three non-N2-fixing reference crops were grown under the same conditions. Regardless of Bradyrhizobium strain, no differences were observed in dry matter, nodule weight and total N between labelled and non-labelled soil. The N2 fixation of the soybeans grown in the two soil conditions were similar. The mean 'B' values of the soybeans inoculated with the B. japonicum strains were -1.84 and -0.50 , while those inoculated with B. elkanii were -3.67 and -1.0 , for the shoot tissue and the whole plant, respectively. Finally, the 'B' value for the soybean crop varied considerably in function of the inoculated Bradyrhizobium strain, being most important when only the shoot tissue was utilised to estimate the proportion of N in the plant derived from N2 fixation. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-90162008000500011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
dc.source.none.fl_str_mv |
Scientia Agricola v.65 n.5 2008 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1748936461035503616 |