Biochemical changes and accumulation in "pacu" fish (Metynnis argenteus) exposed to paclobutrazol
Main Author: | |
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Publication Date: | 2002 |
Other Authors: | , , , |
Format: | Article |
Language: | por |
Source: | Scientia Agrícola (Online) |
Download full: | https://www.revistas.usp.br/sa/article/view/21750 |
Summary: | Paclobutrazol is a plant growth regulator with fungicide properties which is widely used in Brazilian Northeast orchards. This chemical remains stable in the soil for several months and its half-life in water is longer than three weeks. Because of these characteristics, the aquatic contamination by paclobutrazol may occur, potentially harming aquatic fauna and human health. This work evaluates the bioconcentration and the activities of the enzymes: glutamic oxaloacetic transaminase (GOT), lactate dehydrogenase (LDH) and glutathione S-transferase (GST) in the Brazilian fish "pacu prata" (Metynnis argenteus) exposed to paclobutrazol for 28 days. Results showed a significant increase in the enzymatic activity of LDH and GST in liver, for concentrations equals to 3 mg L-1 (28º day exposure) and 30 mg L-1 (7º day exposure), respectively. Paclobutrazol residues in muscle reached 166 mg kg-1 at the end of the period of exposure for the measured concentration in the water equivalent to 2.7 mg L-1. The bioconcentration factor estimated for the steady state condition was 73. It was concluded that the measure of LDH and GST activities could be used as biomarkers of paclobutrazol exposure. This exposure may already be in extreme conditions to cause significant risk to humans consuming contaminated fish. |
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Biochemical changes and accumulation in "pacu" fish (Metynnis argenteus) exposed to paclobutrazol Alterações bioquímicas e acúmulo em pacus (Metynnis argenteus) expostos ao paclobutrazol bioconcentraçãopeixetoxicidadebioconcentrationfishtoxicity Paclobutrazol is a plant growth regulator with fungicide properties which is widely used in Brazilian Northeast orchards. This chemical remains stable in the soil for several months and its half-life in water is longer than three weeks. Because of these characteristics, the aquatic contamination by paclobutrazol may occur, potentially harming aquatic fauna and human health. This work evaluates the bioconcentration and the activities of the enzymes: glutamic oxaloacetic transaminase (GOT), lactate dehydrogenase (LDH) and glutathione S-transferase (GST) in the Brazilian fish "pacu prata" (Metynnis argenteus) exposed to paclobutrazol for 28 days. Results showed a significant increase in the enzymatic activity of LDH and GST in liver, for concentrations equals to 3 mg L-1 (28º day exposure) and 30 mg L-1 (7º day exposure), respectively. Paclobutrazol residues in muscle reached 166 mg kg-1 at the end of the period of exposure for the measured concentration in the water equivalent to 2.7 mg L-1. The bioconcentration factor estimated for the steady state condition was 73. It was concluded that the measure of LDH and GST activities could be used as biomarkers of paclobutrazol exposure. This exposure may already be in extreme conditions to cause significant risk to humans consuming contaminated fish. O paclobutrazol, um regulador de crescimento vegetal com propriedades fungicidas, é muito utilizado na região Nordeste do Brasil em áreas de produção de frutíferas. Este composto pode permanecer estável por vários meses após sua aplicação no solo, sendo sua meia vida na água superior a três semanas. Corpos de água superficiais nas proximidades das culturas são portanto susceptíveis de serem contaminados pelo paclobutrazol, com o risco de promover efeitos adversos para a fauna aquática e para a saúde humana. No presente trabalho foi estudada a bioconcentração e algumas alterações bioquímicas, medidas pela atividade das enzimas transaminase glutâmico-oxalacética (TGO), lactato desidrogenase (LDH) e glutationa S-transferase (GST) em tecidos do peixe pacú prata (Metynnis argenteus), exposto ao paclobutrazol durante 28 dias. O paclobutrazol aumentou a atividade de LDH e GST no fígado para as concentrações de 3 mg L-1 (28º dia de exposição) e 30 mg L-1 (7º dia de exposição), respectivamente. No 28º dia, a concentração de paclobutrazol no músculo atingiu níveis residuais de 166 mg kg-1 para a concentração média determinada na água de 2,7 mg L-1, estimando-se um fator de bioconcentração (FBC) para a situação de equilíbrio equivalente a 73. A medida da atividade de LDH e GST pode ser utilizada como biomarcador de exposição ao paclobutrazol. Esta exposição deve estar em situações extremas para proporcionar risco significativo em humanos que se alimentam de peixes contendo resíduos do agroquímico. Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz2002-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/sa/article/view/2175010.1590/S0103-90162002000300005Scientia Agricola; v. 59 n. 3 (2002); 441-446Scientia Agricola; Vol. 59 No. 3 (2002); 441-446Scientia Agricola; Vol. 59 Núm. 3 (2002); 441-4461678-992X0103-9016reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/sa/article/view/21750/23774Copyright (c) 2015 Scientia Agricolainfo:eu-repo/semantics/openAccessJonsson, Claudio MartínFerracini, Vera LúciaParaíba, Lourival CostaRangel, MaurícioAguiar, Sandra Regina2015-07-07T12:31:46Zoai:revistas.usp.br:article/21750Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2015-07-07T12:31:46Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Biochemical changes and accumulation in "pacu" fish (Metynnis argenteus) exposed to paclobutrazol Alterações bioquímicas e acúmulo em pacus (Metynnis argenteus) expostos ao paclobutrazol |
title |
Biochemical changes and accumulation in "pacu" fish (Metynnis argenteus) exposed to paclobutrazol |
spellingShingle |
Biochemical changes and accumulation in "pacu" fish (Metynnis argenteus) exposed to paclobutrazol Jonsson, Claudio Martín bioconcentração peixe toxicidade bioconcentration fish toxicity |
title_short |
Biochemical changes and accumulation in "pacu" fish (Metynnis argenteus) exposed to paclobutrazol |
title_full |
Biochemical changes and accumulation in "pacu" fish (Metynnis argenteus) exposed to paclobutrazol |
title_fullStr |
Biochemical changes and accumulation in "pacu" fish (Metynnis argenteus) exposed to paclobutrazol |
title_full_unstemmed |
Biochemical changes and accumulation in "pacu" fish (Metynnis argenteus) exposed to paclobutrazol |
title_sort |
Biochemical changes and accumulation in "pacu" fish (Metynnis argenteus) exposed to paclobutrazol |
author |
Jonsson, Claudio Martín |
author_facet |
Jonsson, Claudio Martín Ferracini, Vera Lúcia Paraíba, Lourival Costa Rangel, Maurício Aguiar, Sandra Regina |
author_role |
author |
author2 |
Ferracini, Vera Lúcia Paraíba, Lourival Costa Rangel, Maurício Aguiar, Sandra Regina |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Jonsson, Claudio Martín Ferracini, Vera Lúcia Paraíba, Lourival Costa Rangel, Maurício Aguiar, Sandra Regina |
dc.subject.por.fl_str_mv |
bioconcentração peixe toxicidade bioconcentration fish toxicity |
topic |
bioconcentração peixe toxicidade bioconcentration fish toxicity |
description |
Paclobutrazol is a plant growth regulator with fungicide properties which is widely used in Brazilian Northeast orchards. This chemical remains stable in the soil for several months and its half-life in water is longer than three weeks. Because of these characteristics, the aquatic contamination by paclobutrazol may occur, potentially harming aquatic fauna and human health. This work evaluates the bioconcentration and the activities of the enzymes: glutamic oxaloacetic transaminase (GOT), lactate dehydrogenase (LDH) and glutathione S-transferase (GST) in the Brazilian fish "pacu prata" (Metynnis argenteus) exposed to paclobutrazol for 28 days. Results showed a significant increase in the enzymatic activity of LDH and GST in liver, for concentrations equals to 3 mg L-1 (28º day exposure) and 30 mg L-1 (7º day exposure), respectively. Paclobutrazol residues in muscle reached 166 mg kg-1 at the end of the period of exposure for the measured concentration in the water equivalent to 2.7 mg L-1. The bioconcentration factor estimated for the steady state condition was 73. It was concluded that the measure of LDH and GST activities could be used as biomarkers of paclobutrazol exposure. This exposure may already be in extreme conditions to cause significant risk to humans consuming contaminated fish. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/sa/article/view/21750 10.1590/S0103-90162002000300005 |
url |
https://www.revistas.usp.br/sa/article/view/21750 |
identifier_str_mv |
10.1590/S0103-90162002000300005 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/sa/article/view/21750/23774 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Scientia Agricola info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Scientia Agricola |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz |
publisher.none.fl_str_mv |
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz |
dc.source.none.fl_str_mv |
Scientia Agricola; v. 59 n. 3 (2002); 441-446 Scientia Agricola; Vol. 59 No. 3 (2002); 441-446 Scientia Agricola; Vol. 59 Núm. 3 (2002); 441-446 1678-992X 0103-9016 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1787713253186469888 |