Molecular fingerprinting of Fusarium oxysporum f. sp. passiflorae isolates using AFLP markers
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | https://www.revistas.usp.br/sa/article/view/52991 |
Resumo: | Fusarium oxysporum f. sp. passiflorae W.L. Gordon (FOP) is one of the most important fungal pathogens of passion fruits. Understanding molecular variation of isolates from different areas is of utmost importance. Molecular fingerprinting on 14 isolates of FOP were conducted using AFLP molecular markers (Amplified Fragment Length Polymorphism), and their genetic variability were estimated. Twenty-five AFLP primer combinations were selected for amplification of FOP isolates and one for Fusarium oxysporum f. sp. cubense W.C. Snyder & H.N. Hansen (FOC), resulting in 99% polymorphic fragments, with an average of 40 fragments per primer combination. Specific fingerprints could be generated for most of the isolates evaluated; we observed a high power of discrimination of the AFLP primer combinations, with the presence/absence of up to 26 specific fragments per isolate. Thus, specific fingerprinting was obtained for 10 of the 15 isolates analyzed. The values of the polymorphic information content, the index and the resolving power of the markers showed wide variation and reflected the high informative contents of the primers used in the characterization of the FOP isolates. The FOP isolates were divided into four groups, irrespective of their geographic origins, with the allocation of 5, 7, 1 and 1 FOP isolates into Groups II, III, IV and V, respectively. A wide genetic diversity was observed in FOP isolates, which should be taken into consideration when implementing strategies for the improvement of passion fruit in the search for cultivars with multiple resistance to different isolates. |
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Molecular fingerprinting of Fusarium oxysporum f. sp. passiflorae isolates using AFLP markers passion fruitfusarium wiltfingerprintvariabilitymolecular marker Fusarium oxysporum f. sp. passiflorae W.L. Gordon (FOP) is one of the most important fungal pathogens of passion fruits. Understanding molecular variation of isolates from different areas is of utmost importance. Molecular fingerprinting on 14 isolates of FOP were conducted using AFLP molecular markers (Amplified Fragment Length Polymorphism), and their genetic variability were estimated. Twenty-five AFLP primer combinations were selected for amplification of FOP isolates and one for Fusarium oxysporum f. sp. cubense W.C. Snyder & H.N. Hansen (FOC), resulting in 99% polymorphic fragments, with an average of 40 fragments per primer combination. Specific fingerprints could be generated for most of the isolates evaluated; we observed a high power of discrimination of the AFLP primer combinations, with the presence/absence of up to 26 specific fragments per isolate. Thus, specific fingerprinting was obtained for 10 of the 15 isolates analyzed. The values of the polymorphic information content, the index and the resolving power of the markers showed wide variation and reflected the high informative contents of the primers used in the characterization of the FOP isolates. The FOP isolates were divided into four groups, irrespective of their geographic origins, with the allocation of 5, 7, 1 and 1 FOP isolates into Groups II, III, IV and V, respectively. A wide genetic diversity was observed in FOP isolates, which should be taken into consideration when implementing strategies for the improvement of passion fruit in the search for cultivars with multiple resistance to different isolates. Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz2013-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/sa/article/view/5299110.1590/S0103-90162013000200008Scientia Agricola; v. 70 n. 2 (2013); 108-115Scientia Agricola; Vol. 70 Núm. 2 (2013); 108-115Scientia Agricola; Vol. 70 No. 2 (2013); 108-1151678-992X0103-9016reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/sa/article/view/52991/57047Copyright (c) 2015 Scientia Agricolainfo:eu-repo/semantics/openAccessSilva, Aline dos SantosOliveira, Eder Jorge deHaddad, FernandoJesus, Onildo Nunes deOliveira, Saulo Alves Santos deCosta, Maria Angélica Pereira de Carvalho2015-07-07T19:19:12Zoai:revistas.usp.br:article/52991Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2015-07-07T19:19:12Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Molecular fingerprinting of Fusarium oxysporum f. sp. passiflorae isolates using AFLP markers |
title |
Molecular fingerprinting of Fusarium oxysporum f. sp. passiflorae isolates using AFLP markers |
spellingShingle |
Molecular fingerprinting of Fusarium oxysporum f. sp. passiflorae isolates using AFLP markers Silva, Aline dos Santos passion fruit fusarium wilt fingerprint variability molecular marker |
title_short |
Molecular fingerprinting of Fusarium oxysporum f. sp. passiflorae isolates using AFLP markers |
title_full |
Molecular fingerprinting of Fusarium oxysporum f. sp. passiflorae isolates using AFLP markers |
title_fullStr |
Molecular fingerprinting of Fusarium oxysporum f. sp. passiflorae isolates using AFLP markers |
title_full_unstemmed |
Molecular fingerprinting of Fusarium oxysporum f. sp. passiflorae isolates using AFLP markers |
title_sort |
Molecular fingerprinting of Fusarium oxysporum f. sp. passiflorae isolates using AFLP markers |
author |
Silva, Aline dos Santos |
author_facet |
Silva, Aline dos Santos Oliveira, Eder Jorge de Haddad, Fernando Jesus, Onildo Nunes de Oliveira, Saulo Alves Santos de Costa, Maria Angélica Pereira de Carvalho |
author_role |
author |
author2 |
Oliveira, Eder Jorge de Haddad, Fernando Jesus, Onildo Nunes de Oliveira, Saulo Alves Santos de Costa, Maria Angélica Pereira de Carvalho |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Aline dos Santos Oliveira, Eder Jorge de Haddad, Fernando Jesus, Onildo Nunes de Oliveira, Saulo Alves Santos de Costa, Maria Angélica Pereira de Carvalho |
dc.subject.por.fl_str_mv |
passion fruit fusarium wilt fingerprint variability molecular marker |
topic |
passion fruit fusarium wilt fingerprint variability molecular marker |
description |
Fusarium oxysporum f. sp. passiflorae W.L. Gordon (FOP) is one of the most important fungal pathogens of passion fruits. Understanding molecular variation of isolates from different areas is of utmost importance. Molecular fingerprinting on 14 isolates of FOP were conducted using AFLP molecular markers (Amplified Fragment Length Polymorphism), and their genetic variability were estimated. Twenty-five AFLP primer combinations were selected for amplification of FOP isolates and one for Fusarium oxysporum f. sp. cubense W.C. Snyder & H.N. Hansen (FOC), resulting in 99% polymorphic fragments, with an average of 40 fragments per primer combination. Specific fingerprints could be generated for most of the isolates evaluated; we observed a high power of discrimination of the AFLP primer combinations, with the presence/absence of up to 26 specific fragments per isolate. Thus, specific fingerprinting was obtained for 10 of the 15 isolates analyzed. The values of the polymorphic information content, the index and the resolving power of the markers showed wide variation and reflected the high informative contents of the primers used in the characterization of the FOP isolates. The FOP isolates were divided into four groups, irrespective of their geographic origins, with the allocation of 5, 7, 1 and 1 FOP isolates into Groups II, III, IV and V, respectively. A wide genetic diversity was observed in FOP isolates, which should be taken into consideration when implementing strategies for the improvement of passion fruit in the search for cultivars with multiple resistance to different isolates. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/sa/article/view/52991 10.1590/S0103-90162013000200008 |
url |
https://www.revistas.usp.br/sa/article/view/52991 |
identifier_str_mv |
10.1590/S0103-90162013000200008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/sa/article/view/52991/57047 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Scientia Agricola info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Scientia Agricola |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz |
publisher.none.fl_str_mv |
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz |
dc.source.none.fl_str_mv |
Scientia Agricola; v. 70 n. 2 (2013); 108-115 Scientia Agricola; Vol. 70 Núm. 2 (2013); 108-115 Scientia Agricola; Vol. 70 No. 2 (2013); 108-115 1678-992X 0103-9016 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1800222791941750784 |