Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000500002 |
Resumo: | In the search for new organisms and new secondary metabolites, a study was conducted to evaluate the diversity of endophytic bacteria from ethnovarieties of cassava cultivated by Brazilian Amazon Indian tribes and also to study the secondary metabolites produced by a Bacillus pumilus strain. Sixty seven cassava endophytic bacteria were subjected to 16S rRNA sequencing and FAME analysis. The bacterial profile revealed that 25% of all endophytic isolates belonged to the genus Bacillus. The isolate B. pumilus MAIIIM4a showed a strong inhibitory activity against the fungi Rhizoctonia solani, Pythium aphanidermatum and Sclerotium rolfsii. Secondary metabolites of this strain were extracted using hexane, dichloromethane and ethyl acetate. Extracts were subjected to bioautography and LC/MS analysis, which allowed the identification of pumilacidin, an antifungal compound produced by B. pumilus MAIIIM4a. The bacterial endophytic localization was confirmed by cassava cell tissue examination using scanning electron microscopy. |
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Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4aESI-MS/MSpumilacidinendophytic bacteriabioautographyIn the search for new organisms and new secondary metabolites, a study was conducted to evaluate the diversity of endophytic bacteria from ethnovarieties of cassava cultivated by Brazilian Amazon Indian tribes and also to study the secondary metabolites produced by a Bacillus pumilus strain. Sixty seven cassava endophytic bacteria were subjected to 16S rRNA sequencing and FAME analysis. The bacterial profile revealed that 25% of all endophytic isolates belonged to the genus Bacillus. The isolate B. pumilus MAIIIM4a showed a strong inhibitory activity against the fungi Rhizoctonia solani, Pythium aphanidermatum and Sclerotium rolfsii. Secondary metabolites of this strain were extracted using hexane, dichloromethane and ethyl acetate. Extracts were subjected to bioautography and LC/MS analysis, which allowed the identification of pumilacidin, an antifungal compound produced by B. pumilus MAIIIM4a. The bacterial endophytic localization was confirmed by cassava cell tissue examination using scanning electron microscopy.Escola Superior de Agricultura "Luiz de Queiroz"2009-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000500002Scientia Agricola v.66 n.5 2009reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162009000500002info:eu-repo/semantics/openAccessMelo,Flávia Mandolesi Pereira deFiore,Marli FátimaMoraes,Luiz Alberto Beraldo deSilva-Stenico,Maria EstelaScramin,ShirleiTeixeira,Manoel de AraújoMelo,Itamar Soares deeng2009-10-05T00:00:00Zoai:scielo:S0103-90162009000500002Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2009-10-05T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a |
title |
Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a |
spellingShingle |
Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a Melo,Flávia Mandolesi Pereira de ESI-MS/MS pumilacidin endophytic bacteria bioautography |
title_short |
Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a |
title_full |
Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a |
title_fullStr |
Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a |
title_full_unstemmed |
Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a |
title_sort |
Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a |
author |
Melo,Flávia Mandolesi Pereira de |
author_facet |
Melo,Flávia Mandolesi Pereira de Fiore,Marli Fátima Moraes,Luiz Alberto Beraldo de Silva-Stenico,Maria Estela Scramin,Shirlei Teixeira,Manoel de Araújo Melo,Itamar Soares de |
author_role |
author |
author2 |
Fiore,Marli Fátima Moraes,Luiz Alberto Beraldo de Silva-Stenico,Maria Estela Scramin,Shirlei Teixeira,Manoel de Araújo Melo,Itamar Soares de |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Melo,Flávia Mandolesi Pereira de Fiore,Marli Fátima Moraes,Luiz Alberto Beraldo de Silva-Stenico,Maria Estela Scramin,Shirlei Teixeira,Manoel de Araújo Melo,Itamar Soares de |
dc.subject.por.fl_str_mv |
ESI-MS/MS pumilacidin endophytic bacteria bioautography |
topic |
ESI-MS/MS pumilacidin endophytic bacteria bioautography |
description |
In the search for new organisms and new secondary metabolites, a study was conducted to evaluate the diversity of endophytic bacteria from ethnovarieties of cassava cultivated by Brazilian Amazon Indian tribes and also to study the secondary metabolites produced by a Bacillus pumilus strain. Sixty seven cassava endophytic bacteria were subjected to 16S rRNA sequencing and FAME analysis. The bacterial profile revealed that 25% of all endophytic isolates belonged to the genus Bacillus. The isolate B. pumilus MAIIIM4a showed a strong inhibitory activity against the fungi Rhizoctonia solani, Pythium aphanidermatum and Sclerotium rolfsii. Secondary metabolites of this strain were extracted using hexane, dichloromethane and ethyl acetate. Extracts were subjected to bioautography and LC/MS analysis, which allowed the identification of pumilacidin, an antifungal compound produced by B. pumilus MAIIIM4a. The bacterial endophytic localization was confirmed by cassava cell tissue examination using scanning electron microscopy. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000500002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000500002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-90162009000500002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
dc.source.none.fl_str_mv |
Scientia Agricola v.66 n.5 2009 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1748936461520994304 |