Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a

Detalhes bibliográficos
Autor(a) principal: Melo,Flávia Mandolesi Pereira de
Data de Publicação: 2009
Outros Autores: Fiore,Marli Fátima, Moraes,Luiz Alberto Beraldo de, Silva-Stenico,Maria Estela, Scramin,Shirlei, Teixeira,Manoel de Araújo, Melo,Itamar Soares de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000500002
Resumo: In the search for new organisms and new secondary metabolites, a study was conducted to evaluate the diversity of endophytic bacteria from ethnovarieties of cassava cultivated by Brazilian Amazon Indian tribes and also to study the secondary metabolites produced by a Bacillus pumilus strain. Sixty seven cassava endophytic bacteria were subjected to 16S rRNA sequencing and FAME analysis. The bacterial profile revealed that 25% of all endophytic isolates belonged to the genus Bacillus. The isolate B. pumilus MAIIIM4a showed a strong inhibitory activity against the fungi Rhizoctonia solani, Pythium aphanidermatum and Sclerotium rolfsii. Secondary metabolites of this strain were extracted using hexane, dichloromethane and ethyl acetate. Extracts were subjected to bioautography and LC/MS analysis, which allowed the identification of pumilacidin, an antifungal compound produced by B. pumilus MAIIIM4a. The bacterial endophytic localization was confirmed by cassava cell tissue examination using scanning electron microscopy.
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spelling Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4aESI-MS/MSpumilacidinendophytic bacteriabioautographyIn the search for new organisms and new secondary metabolites, a study was conducted to evaluate the diversity of endophytic bacteria from ethnovarieties of cassava cultivated by Brazilian Amazon Indian tribes and also to study the secondary metabolites produced by a Bacillus pumilus strain. Sixty seven cassava endophytic bacteria were subjected to 16S rRNA sequencing and FAME analysis. The bacterial profile revealed that 25% of all endophytic isolates belonged to the genus Bacillus. The isolate B. pumilus MAIIIM4a showed a strong inhibitory activity against the fungi Rhizoctonia solani, Pythium aphanidermatum and Sclerotium rolfsii. Secondary metabolites of this strain were extracted using hexane, dichloromethane and ethyl acetate. Extracts were subjected to bioautography and LC/MS analysis, which allowed the identification of pumilacidin, an antifungal compound produced by B. pumilus MAIIIM4a. The bacterial endophytic localization was confirmed by cassava cell tissue examination using scanning electron microscopy.Escola Superior de Agricultura "Luiz de Queiroz"2009-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000500002Scientia Agricola v.66 n.5 2009reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162009000500002info:eu-repo/semantics/openAccessMelo,Flávia Mandolesi Pereira deFiore,Marli FátimaMoraes,Luiz Alberto Beraldo deSilva-Stenico,Maria EstelaScramin,ShirleiTeixeira,Manoel de AraújoMelo,Itamar Soares deeng2009-10-05T00:00:00Zoai:scielo:S0103-90162009000500002Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2009-10-05T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a
title Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a
spellingShingle Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a
Melo,Flávia Mandolesi Pereira de
ESI-MS/MS
pumilacidin
endophytic bacteria
bioautography
title_short Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a
title_full Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a
title_fullStr Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a
title_full_unstemmed Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a
title_sort Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a
author Melo,Flávia Mandolesi Pereira de
author_facet Melo,Flávia Mandolesi Pereira de
Fiore,Marli Fátima
Moraes,Luiz Alberto Beraldo de
Silva-Stenico,Maria Estela
Scramin,Shirlei
Teixeira,Manoel de Araújo
Melo,Itamar Soares de
author_role author
author2 Fiore,Marli Fátima
Moraes,Luiz Alberto Beraldo de
Silva-Stenico,Maria Estela
Scramin,Shirlei
Teixeira,Manoel de Araújo
Melo,Itamar Soares de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Melo,Flávia Mandolesi Pereira de
Fiore,Marli Fátima
Moraes,Luiz Alberto Beraldo de
Silva-Stenico,Maria Estela
Scramin,Shirlei
Teixeira,Manoel de Araújo
Melo,Itamar Soares de
dc.subject.por.fl_str_mv ESI-MS/MS
pumilacidin
endophytic bacteria
bioautography
topic ESI-MS/MS
pumilacidin
endophytic bacteria
bioautography
description In the search for new organisms and new secondary metabolites, a study was conducted to evaluate the diversity of endophytic bacteria from ethnovarieties of cassava cultivated by Brazilian Amazon Indian tribes and also to study the secondary metabolites produced by a Bacillus pumilus strain. Sixty seven cassava endophytic bacteria were subjected to 16S rRNA sequencing and FAME analysis. The bacterial profile revealed that 25% of all endophytic isolates belonged to the genus Bacillus. The isolate B. pumilus MAIIIM4a showed a strong inhibitory activity against the fungi Rhizoctonia solani, Pythium aphanidermatum and Sclerotium rolfsii. Secondary metabolites of this strain were extracted using hexane, dichloromethane and ethyl acetate. Extracts were subjected to bioautography and LC/MS analysis, which allowed the identification of pumilacidin, an antifungal compound produced by B. pumilus MAIIIM4a. The bacterial endophytic localization was confirmed by cassava cell tissue examination using scanning electron microscopy.
publishDate 2009
dc.date.none.fl_str_mv 2009-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000500002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000500002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-90162009000500002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
dc.source.none.fl_str_mv Scientia Agricola v.66 n.5 2009
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
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