Identification of the Er1 resistence gene and RNase S-alleles in Malus prunifolia var. ringo rootstock

Detalhes bibliográficos
Autor(a) principal: Agapito-Tenfen,Sarah Zanon
Data de Publicação: 2015
Outros Autores: Dantas,Adriana Cibele de Mesquita, Denardi,Frederico, Nodari,Rubens Onofre
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000100062
Resumo: Woolly apple aphid (WAA; Eriosoma lanigerum Hausm.) is a major insect pest that has significant economic impact on apple growers worldwide. Modern breeding technologies rely on several molecular tools to help breeders select genetic determinants for traits of interest. Consequently, there is a need for specific markers linked to the genes of interest. Apple scions and rootstocks have an additional barrier to the introduction of pest resistance genes due to the presence of self-incompatibility S-RNase alleles. The genetic characterization and early identification of these alleles can amplify the contribution of a breeding program to the selection of resistant genitors that are as compatible as possible. In this study, we identified the Er1 gene involved in the resistance to WAA in Malus prunifolia var. ringo, also known as ‘Maruba Kaido’ rootstock, and we analyzed the inheritance pattern of the WAA resistance Er1 gene in a segregant population derived from Malus pumila ‘M.9’ and ‘Maruba Kaido’ rootstocks. The self-incompatibility of S-RNase alleles S6S26 of ‘Maruba Kaido’ were also identified along with their inheritance pattern. We also confirmed the identification of the S1S3 alleles in the ‘M.9’ rootstock. To the best of our knowledge, this is the first study to characterize WAA resistance and RNase S-alleles in ‘Maruba Kaido’. Furthermore, we discuss the potential use of the genetic markers for these genes and their potential impact on apple breeding programs.
id USP-18_51da55eda8501ee17b929c5f08c55a62
oai_identifier_str oai:scielo:S0103-90162015000100062
network_acronym_str USP-18
network_name_str Scientia Agrícola (Online)
repository_id_str
spelling Identification of the Er1 resistence gene and RNase S-alleles in Malus prunifolia var. ringo rootstockMalus pumilawoolly apple aphidself-incompatibilitybulk segregant analysismarker-assisted selectionWoolly apple aphid (WAA; Eriosoma lanigerum Hausm.) is a major insect pest that has significant economic impact on apple growers worldwide. Modern breeding technologies rely on several molecular tools to help breeders select genetic determinants for traits of interest. Consequently, there is a need for specific markers linked to the genes of interest. Apple scions and rootstocks have an additional barrier to the introduction of pest resistance genes due to the presence of self-incompatibility S-RNase alleles. The genetic characterization and early identification of these alleles can amplify the contribution of a breeding program to the selection of resistant genitors that are as compatible as possible. In this study, we identified the Er1 gene involved in the resistance to WAA in Malus prunifolia var. ringo, also known as ‘Maruba Kaido’ rootstock, and we analyzed the inheritance pattern of the WAA resistance Er1 gene in a segregant population derived from Malus pumila ‘M.9’ and ‘Maruba Kaido’ rootstocks. The self-incompatibility of S-RNase alleles S6S26 of ‘Maruba Kaido’ were also identified along with their inheritance pattern. We also confirmed the identification of the S1S3 alleles in the ‘M.9’ rootstock. To the best of our knowledge, this is the first study to characterize WAA resistance and RNase S-alleles in ‘Maruba Kaido’. Furthermore, we discuss the potential use of the genetic markers for these genes and their potential impact on apple breeding programs.Escola Superior de Agricultura "Luiz de Queiroz"2015-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000100062Scientia Agricola v.72 n.1 2015reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/0103-9016-2013-0210info:eu-repo/semantics/openAccessAgapito-Tenfen,Sarah ZanonDantas,Adriana Cibele de MesquitaDenardi,FredericoNodari,Rubens Onofreeng2015-03-20T00:00:00Zoai:scielo:S0103-90162015000100062Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2015-03-20T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Identification of the Er1 resistence gene and RNase S-alleles in Malus prunifolia var. ringo rootstock
title Identification of the Er1 resistence gene and RNase S-alleles in Malus prunifolia var. ringo rootstock
spellingShingle Identification of the Er1 resistence gene and RNase S-alleles in Malus prunifolia var. ringo rootstock
Agapito-Tenfen,Sarah Zanon
Malus pumila
woolly apple aphid
self-incompatibility
bulk segregant analysis
marker-assisted selection
title_short Identification of the Er1 resistence gene and RNase S-alleles in Malus prunifolia var. ringo rootstock
title_full Identification of the Er1 resistence gene and RNase S-alleles in Malus prunifolia var. ringo rootstock
title_fullStr Identification of the Er1 resistence gene and RNase S-alleles in Malus prunifolia var. ringo rootstock
title_full_unstemmed Identification of the Er1 resistence gene and RNase S-alleles in Malus prunifolia var. ringo rootstock
title_sort Identification of the Er1 resistence gene and RNase S-alleles in Malus prunifolia var. ringo rootstock
author Agapito-Tenfen,Sarah Zanon
author_facet Agapito-Tenfen,Sarah Zanon
Dantas,Adriana Cibele de Mesquita
Denardi,Frederico
Nodari,Rubens Onofre
author_role author
author2 Dantas,Adriana Cibele de Mesquita
Denardi,Frederico
Nodari,Rubens Onofre
author2_role author
author
author
dc.contributor.author.fl_str_mv Agapito-Tenfen,Sarah Zanon
Dantas,Adriana Cibele de Mesquita
Denardi,Frederico
Nodari,Rubens Onofre
dc.subject.por.fl_str_mv Malus pumila
woolly apple aphid
self-incompatibility
bulk segregant analysis
marker-assisted selection
topic Malus pumila
woolly apple aphid
self-incompatibility
bulk segregant analysis
marker-assisted selection
description Woolly apple aphid (WAA; Eriosoma lanigerum Hausm.) is a major insect pest that has significant economic impact on apple growers worldwide. Modern breeding technologies rely on several molecular tools to help breeders select genetic determinants for traits of interest. Consequently, there is a need for specific markers linked to the genes of interest. Apple scions and rootstocks have an additional barrier to the introduction of pest resistance genes due to the presence of self-incompatibility S-RNase alleles. The genetic characterization and early identification of these alleles can amplify the contribution of a breeding program to the selection of resistant genitors that are as compatible as possible. In this study, we identified the Er1 gene involved in the resistance to WAA in Malus prunifolia var. ringo, also known as ‘Maruba Kaido’ rootstock, and we analyzed the inheritance pattern of the WAA resistance Er1 gene in a segregant population derived from Malus pumila ‘M.9’ and ‘Maruba Kaido’ rootstocks. The self-incompatibility of S-RNase alleles S6S26 of ‘Maruba Kaido’ were also identified along with their inheritance pattern. We also confirmed the identification of the S1S3 alleles in the ‘M.9’ rootstock. To the best of our knowledge, this is the first study to characterize WAA resistance and RNase S-alleles in ‘Maruba Kaido’. Furthermore, we discuss the potential use of the genetic markers for these genes and their potential impact on apple breeding programs.
publishDate 2015
dc.date.none.fl_str_mv 2015-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000100062
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000100062
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-9016-2013-0210
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
dc.source.none.fl_str_mv Scientia Agricola v.72 n.1 2015
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
_version_ 1748936463718809600