Low carbon/nitrogen ratio increases laccase production from basidiomycetes in solid substrate cultivation
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000300004 |
Resumo: | Basidiomycetes are laccase producers used for hydrolysis of lignocellulosic byproducts in fermentative processes and could be used on biofuel production or ruminant feeding. The objective of this study was to evaluate the effect of concentrations of non-protein nitrogen sources on laccase production and mycelial growth of Pleurotus ostreatus, Lentinula edodes and Agaricus blazei. The fungi were grown on soybean hulls to which urea (U), ammonium sulfate (AS) or mixture of AS:U (1:1) were added to achieve carbon/nitrogen (C/N) ratios of 5, 15, 20 or 30. The average longitudinal mycelial growth was measured and laccase activity was determined by the oxidation of 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid. Higher C/N ratios increased mycelial growth and decreased laccase production. The highest activities were obtained with a C/N ratio of 5. P. ostreatus, L. edodes and A. blazei produced more laccase when AS, AS:U and U, respectively, were added. In addition, C/N ratios lower than 30 induced laccase syntheses, inhibited mycelial growth and were a better condition for pre-hydrolysis of plant residues. |
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Low carbon/nitrogen ratio increases laccase production from basidiomycetes in solid substrate cultivationindustrial byproductlignocellulolyticenzymelignolyticsoybean hullBasidiomycetes are laccase producers used for hydrolysis of lignocellulosic byproducts in fermentative processes and could be used on biofuel production or ruminant feeding. The objective of this study was to evaluate the effect of concentrations of non-protein nitrogen sources on laccase production and mycelial growth of Pleurotus ostreatus, Lentinula edodes and Agaricus blazei. The fungi were grown on soybean hulls to which urea (U), ammonium sulfate (AS) or mixture of AS:U (1:1) were added to achieve carbon/nitrogen (C/N) ratios of 5, 15, 20 or 30. The average longitudinal mycelial growth was measured and laccase activity was determined by the oxidation of 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid. Higher C/N ratios increased mycelial growth and decreased laccase production. The highest activities were obtained with a C/N ratio of 5. P. ostreatus, L. edodes and A. blazei produced more laccase when AS, AS:U and U, respectively, were added. In addition, C/N ratios lower than 30 induced laccase syntheses, inhibited mycelial growth and were a better condition for pre-hydrolysis of plant residues.Escola Superior de Agricultura "Luiz de Queiroz"2011-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000300004Scientia Agricola v.68 n.3 2011reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162011000300004info:eu-repo/semantics/openAccessD'Agostini,Érica ClarissaMantovani,Talita Rafaele D'AgostiniValle,Juliana Silveira doPaccola-Meirelles,Luzia DorettoColauto,Nelson BarrosLinde,Giani Andreaeng2011-07-11T00:00:00Zoai:scielo:S0103-90162011000300004Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2011-07-11T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Low carbon/nitrogen ratio increases laccase production from basidiomycetes in solid substrate cultivation |
title |
Low carbon/nitrogen ratio increases laccase production from basidiomycetes in solid substrate cultivation |
spellingShingle |
Low carbon/nitrogen ratio increases laccase production from basidiomycetes in solid substrate cultivation D'Agostini,Érica Clarissa industrial byproduct lignocellulolytic enzyme lignolytic soybean hull |
title_short |
Low carbon/nitrogen ratio increases laccase production from basidiomycetes in solid substrate cultivation |
title_full |
Low carbon/nitrogen ratio increases laccase production from basidiomycetes in solid substrate cultivation |
title_fullStr |
Low carbon/nitrogen ratio increases laccase production from basidiomycetes in solid substrate cultivation |
title_full_unstemmed |
Low carbon/nitrogen ratio increases laccase production from basidiomycetes in solid substrate cultivation |
title_sort |
Low carbon/nitrogen ratio increases laccase production from basidiomycetes in solid substrate cultivation |
author |
D'Agostini,Érica Clarissa |
author_facet |
D'Agostini,Érica Clarissa Mantovani,Talita Rafaele D'Agostini Valle,Juliana Silveira do Paccola-Meirelles,Luzia Doretto Colauto,Nelson Barros Linde,Giani Andrea |
author_role |
author |
author2 |
Mantovani,Talita Rafaele D'Agostini Valle,Juliana Silveira do Paccola-Meirelles,Luzia Doretto Colauto,Nelson Barros Linde,Giani Andrea |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
D'Agostini,Érica Clarissa Mantovani,Talita Rafaele D'Agostini Valle,Juliana Silveira do Paccola-Meirelles,Luzia Doretto Colauto,Nelson Barros Linde,Giani Andrea |
dc.subject.por.fl_str_mv |
industrial byproduct lignocellulolytic enzyme lignolytic soybean hull |
topic |
industrial byproduct lignocellulolytic enzyme lignolytic soybean hull |
description |
Basidiomycetes are laccase producers used for hydrolysis of lignocellulosic byproducts in fermentative processes and could be used on biofuel production or ruminant feeding. The objective of this study was to evaluate the effect of concentrations of non-protein nitrogen sources on laccase production and mycelial growth of Pleurotus ostreatus, Lentinula edodes and Agaricus blazei. The fungi were grown on soybean hulls to which urea (U), ammonium sulfate (AS) or mixture of AS:U (1:1) were added to achieve carbon/nitrogen (C/N) ratios of 5, 15, 20 or 30. The average longitudinal mycelial growth was measured and laccase activity was determined by the oxidation of 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid. Higher C/N ratios increased mycelial growth and decreased laccase production. The highest activities were obtained with a C/N ratio of 5. P. ostreatus, L. edodes and A. blazei produced more laccase when AS, AS:U and U, respectively, were added. In addition, C/N ratios lower than 30 induced laccase syntheses, inhibited mycelial growth and were a better condition for pre-hydrolysis of plant residues. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000300004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000300004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-90162011000300004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
dc.source.none.fl_str_mv |
Scientia Agricola v.68 n.3 2011 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1748936462425915392 |