Calcium, potassium and magnesium treatment of Chrysanthemum morifolium cv. "Bi Time" and callogenesis in vitro

Detalhes bibliográficos
Autor(a) principal: Borgatto,Fábio
Data de Publicação: 2002
Outros Autores: Dias,Carlos Tadeu dos Santos, Amaral,Antônio Francisco Campos, Melo,Murilo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000400011
Resumo: The chemical composition and vegetative vigor of the donor plant are essential for the satisfactory performance of explants in vitro. In order to test the effect of potassium, calcium and magnesium nutritional status of Chrysanthemum morifolium plants on callogenesis in vitro, pot plants growing in sand were irrigated with nutrient solution containing different levels of potassium (0; 58.5; 117 and 234 mg L-1), calcium (0; 50; 100 e 200 mg L-1) and magnesium (0;12; 48 e 96 mg L-1). After 30 and 45 days, explants (shoot segments and leaf discs) were collected, desinfected and inoculated on MS solid medium supplemented with 0.1 mg L-1 of kinetin and 5.0 mg L-1 of nafthalene acetic acid for callogenesis induction. Callogenesis evaluated as callus fresh weight was affected by nutrients treatment. Callus growth on leaf explants was inversely proporcional to potassium concentration and directly proportional to magnesium concentration in shoot explants. The calcium effect on callogensis of leaf explants was dependent on treatment duration. For 30 days treatment callogenseis was inversely related to calcium concentration and after 45 days was directly related to calcium concentration.
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spelling Calcium, potassium and magnesium treatment of Chrysanthemum morifolium cv. "Bi Time" and callogenesis in vitromatrix plantmineral nutritiontissue cultureThe chemical composition and vegetative vigor of the donor plant are essential for the satisfactory performance of explants in vitro. In order to test the effect of potassium, calcium and magnesium nutritional status of Chrysanthemum morifolium plants on callogenesis in vitro, pot plants growing in sand were irrigated with nutrient solution containing different levels of potassium (0; 58.5; 117 and 234 mg L-1), calcium (0; 50; 100 e 200 mg L-1) and magnesium (0;12; 48 e 96 mg L-1). After 30 and 45 days, explants (shoot segments and leaf discs) were collected, desinfected and inoculated on MS solid medium supplemented with 0.1 mg L-1 of kinetin and 5.0 mg L-1 of nafthalene acetic acid for callogenesis induction. Callogenesis evaluated as callus fresh weight was affected by nutrients treatment. Callus growth on leaf explants was inversely proporcional to potassium concentration and directly proportional to magnesium concentration in shoot explants. The calcium effect on callogensis of leaf explants was dependent on treatment duration. For 30 days treatment callogenseis was inversely related to calcium concentration and after 45 days was directly related to calcium concentration.Escola Superior de Agricultura "Luiz de Queiroz"2002-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000400011Scientia Agricola v.59 n.4 2002reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162002000400011info:eu-repo/semantics/openAccessBorgatto,FábioDias,Carlos Tadeu dos SantosAmaral,Antônio Francisco CamposMelo,Muriloeng2002-10-16T00:00:00Zoai:scielo:S0103-90162002000400011Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2002-10-16T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Calcium, potassium and magnesium treatment of Chrysanthemum morifolium cv. "Bi Time" and callogenesis in vitro
title Calcium, potassium and magnesium treatment of Chrysanthemum morifolium cv. "Bi Time" and callogenesis in vitro
spellingShingle Calcium, potassium and magnesium treatment of Chrysanthemum morifolium cv. "Bi Time" and callogenesis in vitro
Borgatto,Fábio
matrix plant
mineral nutrition
tissue culture
title_short Calcium, potassium and magnesium treatment of Chrysanthemum morifolium cv. "Bi Time" and callogenesis in vitro
title_full Calcium, potassium and magnesium treatment of Chrysanthemum morifolium cv. "Bi Time" and callogenesis in vitro
title_fullStr Calcium, potassium and magnesium treatment of Chrysanthemum morifolium cv. "Bi Time" and callogenesis in vitro
title_full_unstemmed Calcium, potassium and magnesium treatment of Chrysanthemum morifolium cv. "Bi Time" and callogenesis in vitro
title_sort Calcium, potassium and magnesium treatment of Chrysanthemum morifolium cv. "Bi Time" and callogenesis in vitro
author Borgatto,Fábio
author_facet Borgatto,Fábio
Dias,Carlos Tadeu dos Santos
Amaral,Antônio Francisco Campos
Melo,Murilo
author_role author
author2 Dias,Carlos Tadeu dos Santos
Amaral,Antônio Francisco Campos
Melo,Murilo
author2_role author
author
author
dc.contributor.author.fl_str_mv Borgatto,Fábio
Dias,Carlos Tadeu dos Santos
Amaral,Antônio Francisco Campos
Melo,Murilo
dc.subject.por.fl_str_mv matrix plant
mineral nutrition
tissue culture
topic matrix plant
mineral nutrition
tissue culture
description The chemical composition and vegetative vigor of the donor plant are essential for the satisfactory performance of explants in vitro. In order to test the effect of potassium, calcium and magnesium nutritional status of Chrysanthemum morifolium plants on callogenesis in vitro, pot plants growing in sand were irrigated with nutrient solution containing different levels of potassium (0; 58.5; 117 and 234 mg L-1), calcium (0; 50; 100 e 200 mg L-1) and magnesium (0;12; 48 e 96 mg L-1). After 30 and 45 days, explants (shoot segments and leaf discs) were collected, desinfected and inoculated on MS solid medium supplemented with 0.1 mg L-1 of kinetin and 5.0 mg L-1 of nafthalene acetic acid for callogenesis induction. Callogenesis evaluated as callus fresh weight was affected by nutrients treatment. Callus growth on leaf explants was inversely proporcional to potassium concentration and directly proportional to magnesium concentration in shoot explants. The calcium effect on callogensis of leaf explants was dependent on treatment duration. For 30 days treatment callogenseis was inversely related to calcium concentration and after 45 days was directly related to calcium concentration.
publishDate 2002
dc.date.none.fl_str_mv 2002-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000400011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000400011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-90162002000400011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
dc.source.none.fl_str_mv Scientia Agricola v.59 n.4 2002
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
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