Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500015 |
Resumo: | Exposure to nickel (Ni) at high concentrations can lead to production of reactive oxygen species (ROS) resulting in oxidative damage at the cellular level. We investigated the antioxidative responses of Nicotiana tabacum cv BY-2 cell suspension to Ni stress (0.075 and 0.75 mM NiCl2) over a 72 h period with special attention to potential alterations in isoenzymes of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR). Two main SOD isoenzymes were observed, a Mn-SOD (band I) and a Fe-SOD (band II), as well as one CAT isoenzyme and four GR isoenzymes. Activity staining analysis revealed that CAT activity plays a major role in the early response to Ni-induced oxidative stress, particularly when the Ni concentration used was low, whilst a specific GR isoenzyme appears to respond to the Ni-induced oxidative stress when a much higher Ni concentration was used to induce the stress for the same period of treatment. These results illustrate the importance and advantages of determining individual isoenzyme activities. |
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Scientia Agrícola (Online) |
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|
spelling |
Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension cultureantioxidant enzymescatalasenickelsuperoxide dismutaseExposure to nickel (Ni) at high concentrations can lead to production of reactive oxygen species (ROS) resulting in oxidative damage at the cellular level. We investigated the antioxidative responses of Nicotiana tabacum cv BY-2 cell suspension to Ni stress (0.075 and 0.75 mM NiCl2) over a 72 h period with special attention to potential alterations in isoenzymes of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR). Two main SOD isoenzymes were observed, a Mn-SOD (band I) and a Fe-SOD (band II), as well as one CAT isoenzyme and four GR isoenzymes. Activity staining analysis revealed that CAT activity plays a major role in the early response to Ni-induced oxidative stress, particularly when the Ni concentration used was low, whilst a specific GR isoenzyme appears to respond to the Ni-induced oxidative stress when a much higher Ni concentration was used to induce the stress for the same period of treatment. These results illustrate the importance and advantages of determining individual isoenzyme activities.Escola Superior de Agricultura "Luiz de Queiroz"2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500015Scientia Agricola v.65 n.5 2008reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162008000500015info:eu-repo/semantics/openAccessPompeu,Georgia BertoniGratão,Priscila LupinoVitorello,Victor AlexandreAzevedo,Ricardo Antuneseng2008-09-16T00:00:00Zoai:scielo:S0103-90162008000500015Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2008-09-16T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture |
title |
Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture |
spellingShingle |
Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture Pompeu,Georgia Bertoni antioxidant enzymes catalase nickel superoxide dismutase |
title_short |
Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture |
title_full |
Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture |
title_fullStr |
Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture |
title_full_unstemmed |
Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture |
title_sort |
Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture |
author |
Pompeu,Georgia Bertoni |
author_facet |
Pompeu,Georgia Bertoni Gratão,Priscila Lupino Vitorello,Victor Alexandre Azevedo,Ricardo Antunes |
author_role |
author |
author2 |
Gratão,Priscila Lupino Vitorello,Victor Alexandre Azevedo,Ricardo Antunes |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Pompeu,Georgia Bertoni Gratão,Priscila Lupino Vitorello,Victor Alexandre Azevedo,Ricardo Antunes |
dc.subject.por.fl_str_mv |
antioxidant enzymes catalase nickel superoxide dismutase |
topic |
antioxidant enzymes catalase nickel superoxide dismutase |
description |
Exposure to nickel (Ni) at high concentrations can lead to production of reactive oxygen species (ROS) resulting in oxidative damage at the cellular level. We investigated the antioxidative responses of Nicotiana tabacum cv BY-2 cell suspension to Ni stress (0.075 and 0.75 mM NiCl2) over a 72 h period with special attention to potential alterations in isoenzymes of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR). Two main SOD isoenzymes were observed, a Mn-SOD (band I) and a Fe-SOD (band II), as well as one CAT isoenzyme and four GR isoenzymes. Activity staining analysis revealed that CAT activity plays a major role in the early response to Ni-induced oxidative stress, particularly when the Ni concentration used was low, whilst a specific GR isoenzyme appears to respond to the Ni-induced oxidative stress when a much higher Ni concentration was used to induce the stress for the same period of treatment. These results illustrate the importance and advantages of determining individual isoenzyme activities. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500015 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500015 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-90162008000500015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
dc.source.none.fl_str_mv |
Scientia Agricola v.65 n.5 2008 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1748936461042843648 |