Cytogenetic identification of wheat-Psathyrostachys huashanica amphiploid × triticale progenies for English grain aphid resistance
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000300004 |
Resumo: | English grain aphid (EGA, Sitobion avenae Fabricius) is an important pest in wheat (Triticum aestivum L.). To develop EGA-resistant varieties, introducing the desirable genes from related species is regarded as an efficient avenue. In this study, the F1, F2 and F3 plants derived from the cross of EGA-susceptible wheat-Psathyrostachys huashanica Keng ex Kuo amphiploid (PHW-SA, AABBDDNsNs) and EGA-resistant triticale (Zhongsi 828, AABBRR) were analyzed for EGA resistance. Consequently, PHW-SA was moderately susceptible while Zhongsi 828 and their F1 hybrids were immune, suggesting that the resistance is dominant. All the F2 plants showed high resistance or immunity over two years, indicating that EGA resistance genes are more likely carried by the rye (Secale cereale L.) chromosomes rather than the genomes A or B of Zhongsi 828. In the F3 generation, 25 of 239 lines became susceptible. Giemsa C-banding patterns revealed that these F3 lines had 38-40 chromosomes, including complete rye genome except 5R (and 2R in five lines). Genomic in situ hybridization analysis confirmed this result. During meiosis, all the chromosomes formed bivalents. Six bivalents in 20 lines and five bivalents in five lines were characterized from rye. In contrast, their F2 parental lines had 42 chromosomes (21 bivalents), containing 1R-7R of rye. No P. huashanica chromosomes were detected. Therefore, we propose that the rye chromosome 5R may be related to EGA resistance. |
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Scientia Agrícola (Online) |
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Cytogenetic identification of wheat-Psathyrostachys huashanica amphiploid × triticale progenies for English grain aphid resistancechromosome constitutionsgenetic resourceswide crossEnglish grain aphid (EGA, Sitobion avenae Fabricius) is an important pest in wheat (Triticum aestivum L.). To develop EGA-resistant varieties, introducing the desirable genes from related species is regarded as an efficient avenue. In this study, the F1, F2 and F3 plants derived from the cross of EGA-susceptible wheat-Psathyrostachys huashanica Keng ex Kuo amphiploid (PHW-SA, AABBDDNsNs) and EGA-resistant triticale (Zhongsi 828, AABBRR) were analyzed for EGA resistance. Consequently, PHW-SA was moderately susceptible while Zhongsi 828 and their F1 hybrids were immune, suggesting that the resistance is dominant. All the F2 plants showed high resistance or immunity over two years, indicating that EGA resistance genes are more likely carried by the rye (Secale cereale L.) chromosomes rather than the genomes A or B of Zhongsi 828. In the F3 generation, 25 of 239 lines became susceptible. Giemsa C-banding patterns revealed that these F3 lines had 38-40 chromosomes, including complete rye genome except 5R (and 2R in five lines). Genomic in situ hybridization analysis confirmed this result. During meiosis, all the chromosomes formed bivalents. Six bivalents in 20 lines and five bivalents in five lines were characterized from rye. In contrast, their F2 parental lines had 42 chromosomes (21 bivalents), containing 1R-7R of rye. No P. huashanica chromosomes were detected. Therefore, we propose that the rye chromosome 5R may be related to EGA resistance.Escola Superior de Agricultura "Luiz de Queiroz"2013-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000300004Scientia Agricola v.70 n.3 2013reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162013000300004info:eu-repo/semantics/openAccessXie,QuanKang,HouyangSparkes,Debbie LouiseTao,ShanHu,ZhiqinXu,LiliFan,XingSha,LinaZhang,HaiqinWang,YiZeng,JianZhou,Yonghongeng2013-05-14T00:00:00Zoai:scielo:S0103-90162013000300004Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2013-05-14T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Cytogenetic identification of wheat-Psathyrostachys huashanica amphiploid × triticale progenies for English grain aphid resistance |
title |
Cytogenetic identification of wheat-Psathyrostachys huashanica amphiploid × triticale progenies for English grain aphid resistance |
spellingShingle |
Cytogenetic identification of wheat-Psathyrostachys huashanica amphiploid × triticale progenies for English grain aphid resistance Xie,Quan chromosome constitutions genetic resources wide cross |
title_short |
Cytogenetic identification of wheat-Psathyrostachys huashanica amphiploid × triticale progenies for English grain aphid resistance |
title_full |
Cytogenetic identification of wheat-Psathyrostachys huashanica amphiploid × triticale progenies for English grain aphid resistance |
title_fullStr |
Cytogenetic identification of wheat-Psathyrostachys huashanica amphiploid × triticale progenies for English grain aphid resistance |
title_full_unstemmed |
Cytogenetic identification of wheat-Psathyrostachys huashanica amphiploid × triticale progenies for English grain aphid resistance |
title_sort |
Cytogenetic identification of wheat-Psathyrostachys huashanica amphiploid × triticale progenies for English grain aphid resistance |
author |
Xie,Quan |
author_facet |
Xie,Quan Kang,Houyang Sparkes,Debbie Louise Tao,Shan Hu,Zhiqin Xu,Lili Fan,Xing Sha,Lina Zhang,Haiqin Wang,Yi Zeng,Jian Zhou,Yonghong |
author_role |
author |
author2 |
Kang,Houyang Sparkes,Debbie Louise Tao,Shan Hu,Zhiqin Xu,Lili Fan,Xing Sha,Lina Zhang,Haiqin Wang,Yi Zeng,Jian Zhou,Yonghong |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Xie,Quan Kang,Houyang Sparkes,Debbie Louise Tao,Shan Hu,Zhiqin Xu,Lili Fan,Xing Sha,Lina Zhang,Haiqin Wang,Yi Zeng,Jian Zhou,Yonghong |
dc.subject.por.fl_str_mv |
chromosome constitutions genetic resources wide cross |
topic |
chromosome constitutions genetic resources wide cross |
description |
English grain aphid (EGA, Sitobion avenae Fabricius) is an important pest in wheat (Triticum aestivum L.). To develop EGA-resistant varieties, introducing the desirable genes from related species is regarded as an efficient avenue. In this study, the F1, F2 and F3 plants derived from the cross of EGA-susceptible wheat-Psathyrostachys huashanica Keng ex Kuo amphiploid (PHW-SA, AABBDDNsNs) and EGA-resistant triticale (Zhongsi 828, AABBRR) were analyzed for EGA resistance. Consequently, PHW-SA was moderately susceptible while Zhongsi 828 and their F1 hybrids were immune, suggesting that the resistance is dominant. All the F2 plants showed high resistance or immunity over two years, indicating that EGA resistance genes are more likely carried by the rye (Secale cereale L.) chromosomes rather than the genomes A or B of Zhongsi 828. In the F3 generation, 25 of 239 lines became susceptible. Giemsa C-banding patterns revealed that these F3 lines had 38-40 chromosomes, including complete rye genome except 5R (and 2R in five lines). Genomic in situ hybridization analysis confirmed this result. During meiosis, all the chromosomes formed bivalents. Six bivalents in 20 lines and five bivalents in five lines were characterized from rye. In contrast, their F2 parental lines had 42 chromosomes (21 bivalents), containing 1R-7R of rye. No P. huashanica chromosomes were detected. Therefore, we propose that the rye chromosome 5R may be related to EGA resistance. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000300004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000300004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-90162013000300004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
dc.source.none.fl_str_mv |
Scientia Agricola v.70 n.3 2013 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1748936462950203392 |