Reaction of sources of resistance to white mold to microsatellite haplotypes of Sclerotinia sclerotiorum
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | https://www.revistas.usp.br/sa/article/view/113408 |
Resumo: | White mold caused by the fungus Sclerotinia sclerotiorum is the most yield-limiting disease of common bean in Brazil. To date, there has been no commercial cultivar resistant to this disease. In a greenhouse we evaluated white mold resistance sources (Cornell 605, A195 and G122) against eight isolates of S. sclerotiorum from five Brazilian states. A Brazilian cultivar (BRSMG Madrepérola) and a susceptible check (Beryl) were used as control. Treatments were arranged in factorial combinations (5 × 8) in a completely random design with four replicates. Disease severity was assessed on a rating scale of 1-to-9 together with lesion length, which was used to determine an area under the disease progress curve (AUDPC). Polymorphisms detected in ten microsatellite loci were used to assess variability between the isolates. Each isolate was a distinct haplotype; they formed a genetic tree with two clusters. One cluster was formed by three isolates collected from the states of Minas Gerais and São Paulo (southeastern); the others, by isolates from Paraná, Santa Catarina (southern), Goiás (Mid-western), and again, Minas Gerais. Genotype × isolate interaction was significant. In general, Beryl was more susceptible than BRSMG Madrepérola. Considering the AUDPC and/or the white mold reaction score, Cornell 605 exhibited more physiological resistance than BRSMG Madrepérola to seven isolates, A195 to five isolates, and G122 to two isolates. Our results suggest that Cornell 605 is the best source of resistance to white mold for the southern region, whereas Cornell 605 and A195 are somewhat superior to G122 for the southeastern and mid-western regions. |
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Scientia Agrícola (Online) |
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Reaction of sources of resistance to white mold to microsatellite haplotypes of Sclerotinia sclerotiorum White mold caused by the fungus Sclerotinia sclerotiorum is the most yield-limiting disease of common bean in Brazil. To date, there has been no commercial cultivar resistant to this disease. In a greenhouse we evaluated white mold resistance sources (Cornell 605, A195 and G122) against eight isolates of S. sclerotiorum from five Brazilian states. A Brazilian cultivar (BRSMG Madrepérola) and a susceptible check (Beryl) were used as control. Treatments were arranged in factorial combinations (5 × 8) in a completely random design with four replicates. Disease severity was assessed on a rating scale of 1-to-9 together with lesion length, which was used to determine an area under the disease progress curve (AUDPC). Polymorphisms detected in ten microsatellite loci were used to assess variability between the isolates. Each isolate was a distinct haplotype; they formed a genetic tree with two clusters. One cluster was formed by three isolates collected from the states of Minas Gerais and São Paulo (southeastern); the others, by isolates from Paraná, Santa Catarina (southern), Goiás (Mid-western), and again, Minas Gerais. Genotype × isolate interaction was significant. In general, Beryl was more susceptible than BRSMG Madrepérola. Considering the AUDPC and/or the white mold reaction score, Cornell 605 exhibited more physiological resistance than BRSMG Madrepérola to seven isolates, A195 to five isolates, and G122 to two isolates. Our results suggest that Cornell 605 is the best source of resistance to white mold for the southern region, whereas Cornell 605 and A195 are somewhat superior to G122 for the southeastern and mid-western regions. Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz2016-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/sa/article/view/11340810.1590/0103-9016-2015-0085Scientia Agricola; v. 73 n. 2 (2016); 184-188Scientia Agricola; Vol. 73 Núm. 2 (2016); 184-188Scientia Agricola; Vol. 73 No. 2 (2016); 184-1881678-992X0103-9016reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/sa/article/view/113408/111371Copyright (c) 2016 Scientia Agricolainfo:eu-repo/semantics/openAccessLehner, Miller da SilvaPaula Júnior, Trazilbo José deVieira, Rogério FariaLima, Renan CardosoSoares, Bruno de AlmeidaSilva, Rhaphael Alves2016-03-28T20:23:53Zoai:revistas.usp.br:article/113408Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2016-03-28T20:23:53Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Reaction of sources of resistance to white mold to microsatellite haplotypes of Sclerotinia sclerotiorum |
title |
Reaction of sources of resistance to white mold to microsatellite haplotypes of Sclerotinia sclerotiorum |
spellingShingle |
Reaction of sources of resistance to white mold to microsatellite haplotypes of Sclerotinia sclerotiorum Lehner, Miller da Silva |
title_short |
Reaction of sources of resistance to white mold to microsatellite haplotypes of Sclerotinia sclerotiorum |
title_full |
Reaction of sources of resistance to white mold to microsatellite haplotypes of Sclerotinia sclerotiorum |
title_fullStr |
Reaction of sources of resistance to white mold to microsatellite haplotypes of Sclerotinia sclerotiorum |
title_full_unstemmed |
Reaction of sources of resistance to white mold to microsatellite haplotypes of Sclerotinia sclerotiorum |
title_sort |
Reaction of sources of resistance to white mold to microsatellite haplotypes of Sclerotinia sclerotiorum |
author |
Lehner, Miller da Silva |
author_facet |
Lehner, Miller da Silva Paula Júnior, Trazilbo José de Vieira, Rogério Faria Lima, Renan Cardoso Soares, Bruno de Almeida Silva, Rhaphael Alves |
author_role |
author |
author2 |
Paula Júnior, Trazilbo José de Vieira, Rogério Faria Lima, Renan Cardoso Soares, Bruno de Almeida Silva, Rhaphael Alves |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Lehner, Miller da Silva Paula Júnior, Trazilbo José de Vieira, Rogério Faria Lima, Renan Cardoso Soares, Bruno de Almeida Silva, Rhaphael Alves |
description |
White mold caused by the fungus Sclerotinia sclerotiorum is the most yield-limiting disease of common bean in Brazil. To date, there has been no commercial cultivar resistant to this disease. In a greenhouse we evaluated white mold resistance sources (Cornell 605, A195 and G122) against eight isolates of S. sclerotiorum from five Brazilian states. A Brazilian cultivar (BRSMG Madrepérola) and a susceptible check (Beryl) were used as control. Treatments were arranged in factorial combinations (5 × 8) in a completely random design with four replicates. Disease severity was assessed on a rating scale of 1-to-9 together with lesion length, which was used to determine an area under the disease progress curve (AUDPC). Polymorphisms detected in ten microsatellite loci were used to assess variability between the isolates. Each isolate was a distinct haplotype; they formed a genetic tree with two clusters. One cluster was formed by three isolates collected from the states of Minas Gerais and São Paulo (southeastern); the others, by isolates from Paraná, Santa Catarina (southern), Goiás (Mid-western), and again, Minas Gerais. Genotype × isolate interaction was significant. In general, Beryl was more susceptible than BRSMG Madrepérola. Considering the AUDPC and/or the white mold reaction score, Cornell 605 exhibited more physiological resistance than BRSMG Madrepérola to seven isolates, A195 to five isolates, and G122 to two isolates. Our results suggest that Cornell 605 is the best source of resistance to white mold for the southern region, whereas Cornell 605 and A195 are somewhat superior to G122 for the southeastern and mid-western regions. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/sa/article/view/113408 10.1590/0103-9016-2015-0085 |
url |
https://www.revistas.usp.br/sa/article/view/113408 |
identifier_str_mv |
10.1590/0103-9016-2015-0085 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/sa/article/view/113408/111371 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2016 Scientia Agricola info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2016 Scientia Agricola |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz |
publisher.none.fl_str_mv |
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz |
dc.source.none.fl_str_mv |
Scientia Agricola; v. 73 n. 2 (2016); 184-188 Scientia Agricola; Vol. 73 Núm. 2 (2016); 184-188 Scientia Agricola; Vol. 73 No. 2 (2016); 184-188 1678-992X 0103-9016 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1800222792824651776 |