Optimization of oxalic acid production by fungi for biotechnological solubilization of rock phosphate
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162023000100203 |
Resumo: | ABSTRACT: This study describes a biotechnological strategy for producing and applying oxalic acid to solubilize phosphorus (P) from rock phosphate (RP). We evaluated six fungal species (Aspergillus niger FS1, Penicillium islandicum FS41, Pleurotus ostreatus PO1, Rhizoctonia solani Rhiz555, Sclerotium rolfsii Sr25, and Sclerotinia sclerotiorum Scl134) and three culture media (potato dextrose broth, Tsao and Strasser media) to maximize oxalic acid production. Among the fungal isolates tested and culture media, S. rolfsii Sr25 and Tsao medium showed efficient oxalic acid production. Tsao medium was optimized following a response surface methodology after initial screening of factors affecting RP solubilization. The optimized concentrations were 1 g L–1 NaNO3, 100 g L–1 glucose, 2 g L–1 KH2PO4, 4.5 g L–1 yeast extract, and 25 mg L–1 MgSO4·7H2O used for 20 days of incubation. Under these conditions, 71 mmol L–1 oxalic acid was obtained, representing a three-fold increase over production under non-optimized conditions (20 mmol L–1). Under optimized conditions, oxalic acid produced by S. rolfsii Sr25 reacted with low-solubility RP and solubilized 100 % of the P contained in ore. Thus, using S. rolfsii Sr25 to produce oxalic acid seems a promising biotechnological alternative for P solubilization from RP. |
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Optimization of oxalic acid production by fungi for biotechnological solubilization of rock phosphatephosphorusmicroorganismoxalatefertilizerABSTRACT: This study describes a biotechnological strategy for producing and applying oxalic acid to solubilize phosphorus (P) from rock phosphate (RP). We evaluated six fungal species (Aspergillus niger FS1, Penicillium islandicum FS41, Pleurotus ostreatus PO1, Rhizoctonia solani Rhiz555, Sclerotium rolfsii Sr25, and Sclerotinia sclerotiorum Scl134) and three culture media (potato dextrose broth, Tsao and Strasser media) to maximize oxalic acid production. Among the fungal isolates tested and culture media, S. rolfsii Sr25 and Tsao medium showed efficient oxalic acid production. Tsao medium was optimized following a response surface methodology after initial screening of factors affecting RP solubilization. The optimized concentrations were 1 g L–1 NaNO3, 100 g L–1 glucose, 2 g L–1 KH2PO4, 4.5 g L–1 yeast extract, and 25 mg L–1 MgSO4·7H2O used for 20 days of incubation. Under these conditions, 71 mmol L–1 oxalic acid was obtained, representing a three-fold increase over production under non-optimized conditions (20 mmol L–1). Under optimized conditions, oxalic acid produced by S. rolfsii Sr25 reacted with low-solubility RP and solubilized 100 % of the P contained in ore. Thus, using S. rolfsii Sr25 to produce oxalic acid seems a promising biotechnological alternative for P solubilization from RP.Escola Superior de Agricultura "Luiz de Queiroz"2023-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162023000100203Scientia Agricola v.80 2023reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-992x-2021-0076info:eu-repo/semantics/openAccessAmaro,Jeniffer Kelly CortesXavier,Laura VieiraRibeiro,Michelle Miranda Araújo de CarvalhoVieira,Bruno SérgioMendes,Gilberto de Oliveiraeng2022-05-09T00:00:00Zoai:scielo:S0103-90162023000100203Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2022-05-09T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Optimization of oxalic acid production by fungi for biotechnological solubilization of rock phosphate |
title |
Optimization of oxalic acid production by fungi for biotechnological solubilization of rock phosphate |
spellingShingle |
Optimization of oxalic acid production by fungi for biotechnological solubilization of rock phosphate Amaro,Jeniffer Kelly Cortes phosphorus microorganism oxalate fertilizer |
title_short |
Optimization of oxalic acid production by fungi for biotechnological solubilization of rock phosphate |
title_full |
Optimization of oxalic acid production by fungi for biotechnological solubilization of rock phosphate |
title_fullStr |
Optimization of oxalic acid production by fungi for biotechnological solubilization of rock phosphate |
title_full_unstemmed |
Optimization of oxalic acid production by fungi for biotechnological solubilization of rock phosphate |
title_sort |
Optimization of oxalic acid production by fungi for biotechnological solubilization of rock phosphate |
author |
Amaro,Jeniffer Kelly Cortes |
author_facet |
Amaro,Jeniffer Kelly Cortes Xavier,Laura Vieira Ribeiro,Michelle Miranda Araújo de Carvalho Vieira,Bruno Sérgio Mendes,Gilberto de Oliveira |
author_role |
author |
author2 |
Xavier,Laura Vieira Ribeiro,Michelle Miranda Araújo de Carvalho Vieira,Bruno Sérgio Mendes,Gilberto de Oliveira |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Amaro,Jeniffer Kelly Cortes Xavier,Laura Vieira Ribeiro,Michelle Miranda Araújo de Carvalho Vieira,Bruno Sérgio Mendes,Gilberto de Oliveira |
dc.subject.por.fl_str_mv |
phosphorus microorganism oxalate fertilizer |
topic |
phosphorus microorganism oxalate fertilizer |
description |
ABSTRACT: This study describes a biotechnological strategy for producing and applying oxalic acid to solubilize phosphorus (P) from rock phosphate (RP). We evaluated six fungal species (Aspergillus niger FS1, Penicillium islandicum FS41, Pleurotus ostreatus PO1, Rhizoctonia solani Rhiz555, Sclerotium rolfsii Sr25, and Sclerotinia sclerotiorum Scl134) and three culture media (potato dextrose broth, Tsao and Strasser media) to maximize oxalic acid production. Among the fungal isolates tested and culture media, S. rolfsii Sr25 and Tsao medium showed efficient oxalic acid production. Tsao medium was optimized following a response surface methodology after initial screening of factors affecting RP solubilization. The optimized concentrations were 1 g L–1 NaNO3, 100 g L–1 glucose, 2 g L–1 KH2PO4, 4.5 g L–1 yeast extract, and 25 mg L–1 MgSO4·7H2O used for 20 days of incubation. Under these conditions, 71 mmol L–1 oxalic acid was obtained, representing a three-fold increase over production under non-optimized conditions (20 mmol L–1). Under optimized conditions, oxalic acid produced by S. rolfsii Sr25 reacted with low-solubility RP and solubilized 100 % of the P contained in ore. Thus, using S. rolfsii Sr25 to produce oxalic acid seems a promising biotechnological alternative for P solubilization from RP. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162023000100203 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162023000100203 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-992x-2021-0076 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
dc.source.none.fl_str_mv |
Scientia Agricola v.80 2023 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1748936466138923008 |