Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400010 |
Resumo: | Stresses such as cold and drought can impair plant yield and induce a highly complex array of responses. Sugarcane (Saccharum spp.) is cultivated in tropical and subtropical areas and is considered a cold-sensitive plant. We previously showed that cold stress induces the expression of several genes in in vitro sugarcane plantlets. Here we characterize one of those genes, SsNAC23, a member of the NAC family of plant-specific transcription factors, which are induced by low temperature and other stresses in several plant species. The expression of SsNAC23 was induced in sugarcane plants exposed to low temperatures (4ºC). With the aim of further understanding the regulatory network in response to stress, we used the yeast two-hybrid system to identify sugarcane proteins that interact with SsNAC23. Using SsNAC23 as bait, we screened a cDNA expression library of sugarcane plants submitted to 4ºC for 48 h. Several interacting partners were identified, including stress-related proteins, increasing our knowledge on how sugarcane plants respond to cold stress. One of these interacting partners, a thioredoxin h1, offers insights into the regulation of SsNAC23 activity. |
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Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plantsSsNAC, in situ hybridizationcold stressyeast two hybridStresses such as cold and drought can impair plant yield and induce a highly complex array of responses. Sugarcane (Saccharum spp.) is cultivated in tropical and subtropical areas and is considered a cold-sensitive plant. We previously showed that cold stress induces the expression of several genes in in vitro sugarcane plantlets. Here we characterize one of those genes, SsNAC23, a member of the NAC family of plant-specific transcription factors, which are induced by low temperature and other stresses in several plant species. The expression of SsNAC23 was induced in sugarcane plants exposed to low temperatures (4ºC). With the aim of further understanding the regulatory network in response to stress, we used the yeast two-hybrid system to identify sugarcane proteins that interact with SsNAC23. Using SsNAC23 as bait, we screened a cDNA expression library of sugarcane plants submitted to 4ºC for 48 h. Several interacting partners were identified, including stress-related proteins, increasing our knowledge on how sugarcane plants respond to cold stress. One of these interacting partners, a thioredoxin h1, offers insights into the regulation of SsNAC23 activity.Escola Superior de Agricultura "Luiz de Queiroz"2011-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400010Scientia Agricola v.68 n.4 2011reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162011000400010info:eu-repo/semantics/openAccessDitt,Renata FavaGentile,AgustinaTavares,Rafael GarciaCamargo,Sandra RodriguezFernandez,Jorge HernandezSilva,Marcio Jose daMenossi,Marceloeng2011-09-22T00:00:00Zoai:scielo:S0103-90162011000400010Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2011-09-22T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants |
title |
Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants |
spellingShingle |
Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants Ditt,Renata Fava SsNAC, in situ hybridization cold stress yeast two hybrid |
title_short |
Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants |
title_full |
Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants |
title_fullStr |
Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants |
title_full_unstemmed |
Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants |
title_sort |
Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants |
author |
Ditt,Renata Fava |
author_facet |
Ditt,Renata Fava Gentile,Agustina Tavares,Rafael Garcia Camargo,Sandra Rodriguez Fernandez,Jorge Hernandez Silva,Marcio Jose da Menossi,Marcelo |
author_role |
author |
author2 |
Gentile,Agustina Tavares,Rafael Garcia Camargo,Sandra Rodriguez Fernandez,Jorge Hernandez Silva,Marcio Jose da Menossi,Marcelo |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Ditt,Renata Fava Gentile,Agustina Tavares,Rafael Garcia Camargo,Sandra Rodriguez Fernandez,Jorge Hernandez Silva,Marcio Jose da Menossi,Marcelo |
dc.subject.por.fl_str_mv |
SsNAC, in situ hybridization cold stress yeast two hybrid |
topic |
SsNAC, in situ hybridization cold stress yeast two hybrid |
description |
Stresses such as cold and drought can impair plant yield and induce a highly complex array of responses. Sugarcane (Saccharum spp.) is cultivated in tropical and subtropical areas and is considered a cold-sensitive plant. We previously showed that cold stress induces the expression of several genes in in vitro sugarcane plantlets. Here we characterize one of those genes, SsNAC23, a member of the NAC family of plant-specific transcription factors, which are induced by low temperature and other stresses in several plant species. The expression of SsNAC23 was induced in sugarcane plants exposed to low temperatures (4ºC). With the aim of further understanding the regulatory network in response to stress, we used the yeast two-hybrid system to identify sugarcane proteins that interact with SsNAC23. Using SsNAC23 as bait, we screened a cDNA expression library of sugarcane plants submitted to 4ºC for 48 h. Several interacting partners were identified, including stress-related proteins, increasing our knowledge on how sugarcane plants respond to cold stress. One of these interacting partners, a thioredoxin h1, offers insights into the regulation of SsNAC23 activity. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400010 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-90162011000400010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
dc.source.none.fl_str_mv |
Scientia Agricola v.68 n.4 2011 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1748936462465761280 |