Polyphasic analysis of Acidovorax citrulli strains from northeastern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000300252 |
Resumo: | ABSTRACT Bacterial fruit blotch (BFB) of cucurbit plants is caused by Acidovorax citrulli and represents a serious concern to melon (Cucumis melo L.) growers worldwide, including those in Brazil. Thirty-four A. citrulli strains from different melon production areas of northeastern Brazil were characterized for their virulence on melon fruits and their substrate utilization and molecular profiles. Based on the analysis of BFB severity on melon fruits, the A. citrulli strains were divided into three groups, classified as mildly, moderately or highly virulent. Although host-related groups were not observed, the watermelon and ‘melão-pepino’ strains exhibited only low or moderate virulence on melon fruit. Substrate utilization profiles revealed that 94 % of the 95 tested compounds were used by A. citrulli strains as a carbon source. Overall, based on substrate utilization, low variability was observed with no relationship to host of origin. The formation of one group of A. citrulli strains based on Repetitive Sequence-based PCR (rep-PCR) analysis confirmed the low variability observed in the substrate utilization analyses. Bayesian inference based on the analysis of 23S rDNA partial sequence data resulted in one well-supported clade and clustered the strains with the A. citrulli-type species with high posterior probability support. Based on the markers used, the Brazilian A. citrulli strains belong to a single group, which corresponds to the previously described Group I for this bacterium in the United States. |
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Polyphasic analysis of Acidovorax citrulli strains from northeastern BrazilCucumis melobacterial fruit blotchvariabilityABSTRACT Bacterial fruit blotch (BFB) of cucurbit plants is caused by Acidovorax citrulli and represents a serious concern to melon (Cucumis melo L.) growers worldwide, including those in Brazil. Thirty-four A. citrulli strains from different melon production areas of northeastern Brazil were characterized for their virulence on melon fruits and their substrate utilization and molecular profiles. Based on the analysis of BFB severity on melon fruits, the A. citrulli strains were divided into three groups, classified as mildly, moderately or highly virulent. Although host-related groups were not observed, the watermelon and ‘melão-pepino’ strains exhibited only low or moderate virulence on melon fruit. Substrate utilization profiles revealed that 94 % of the 95 tested compounds were used by A. citrulli strains as a carbon source. Overall, based on substrate utilization, low variability was observed with no relationship to host of origin. The formation of one group of A. citrulli strains based on Repetitive Sequence-based PCR (rep-PCR) analysis confirmed the low variability observed in the substrate utilization analyses. Bayesian inference based on the analysis of 23S rDNA partial sequence data resulted in one well-supported clade and clustered the strains with the A. citrulli-type species with high posterior probability support. Based on the markers used, the Brazilian A. citrulli strains belong to a single group, which corresponds to the previously described Group I for this bacterium in the United States.Escola Superior de Agricultura "Luiz de Queiroz"2016-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000300252Scientia Agricola v.73 n.3 2016reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/0103-9016-2015-0088info:eu-repo/semantics/openAccessSilva,Kirley Michele MarquesXavier,André SilvaGama,Marco Aurélio SiqueiraLima,Nelson BernardiLyra,Maria do Carmo Castanho PereiraMariano,Rosa Lima RamosSouza,Elineide Barbosaeng2016-05-16T00:00:00Zoai:scielo:S0103-90162016000300252Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2016-05-16T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Polyphasic analysis of Acidovorax citrulli strains from northeastern Brazil |
title |
Polyphasic analysis of Acidovorax citrulli strains from northeastern Brazil |
spellingShingle |
Polyphasic analysis of Acidovorax citrulli strains from northeastern Brazil Silva,Kirley Michele Marques Cucumis melo bacterial fruit blotch variability |
title_short |
Polyphasic analysis of Acidovorax citrulli strains from northeastern Brazil |
title_full |
Polyphasic analysis of Acidovorax citrulli strains from northeastern Brazil |
title_fullStr |
Polyphasic analysis of Acidovorax citrulli strains from northeastern Brazil |
title_full_unstemmed |
Polyphasic analysis of Acidovorax citrulli strains from northeastern Brazil |
title_sort |
Polyphasic analysis of Acidovorax citrulli strains from northeastern Brazil |
author |
Silva,Kirley Michele Marques |
author_facet |
Silva,Kirley Michele Marques Xavier,André Silva Gama,Marco Aurélio Siqueira Lima,Nelson Bernardi Lyra,Maria do Carmo Castanho Pereira Mariano,Rosa Lima Ramos Souza,Elineide Barbosa |
author_role |
author |
author2 |
Xavier,André Silva Gama,Marco Aurélio Siqueira Lima,Nelson Bernardi Lyra,Maria do Carmo Castanho Pereira Mariano,Rosa Lima Ramos Souza,Elineide Barbosa |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Silva,Kirley Michele Marques Xavier,André Silva Gama,Marco Aurélio Siqueira Lima,Nelson Bernardi Lyra,Maria do Carmo Castanho Pereira Mariano,Rosa Lima Ramos Souza,Elineide Barbosa |
dc.subject.por.fl_str_mv |
Cucumis melo bacterial fruit blotch variability |
topic |
Cucumis melo bacterial fruit blotch variability |
description |
ABSTRACT Bacterial fruit blotch (BFB) of cucurbit plants is caused by Acidovorax citrulli and represents a serious concern to melon (Cucumis melo L.) growers worldwide, including those in Brazil. Thirty-four A. citrulli strains from different melon production areas of northeastern Brazil were characterized for their virulence on melon fruits and their substrate utilization and molecular profiles. Based on the analysis of BFB severity on melon fruits, the A. citrulli strains were divided into three groups, classified as mildly, moderately or highly virulent. Although host-related groups were not observed, the watermelon and ‘melão-pepino’ strains exhibited only low or moderate virulence on melon fruit. Substrate utilization profiles revealed that 94 % of the 95 tested compounds were used by A. citrulli strains as a carbon source. Overall, based on substrate utilization, low variability was observed with no relationship to host of origin. The formation of one group of A. citrulli strains based on Repetitive Sequence-based PCR (rep-PCR) analysis confirmed the low variability observed in the substrate utilization analyses. Bayesian inference based on the analysis of 23S rDNA partial sequence data resulted in one well-supported clade and clustered the strains with the A. citrulli-type species with high posterior probability support. Based on the markers used, the Brazilian A. citrulli strains belong to a single group, which corresponds to the previously described Group I for this bacterium in the United States. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000300252 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000300252 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-9016-2015-0088 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
dc.source.none.fl_str_mv |
Scientia Agricola v.73 n.3 2016 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1748936463890776064 |