Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae

Detalhes bibliográficos
Autor(a) principal: Kava - Cordeiro,V.
Data de Publicação: 1995
Outros Autores: Luna - Alves - Lima,E.A., Azevedo,J.L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161995000300023
Resumo: A wild strain of Metarhizium anisopliae, an entomopathogenic fungus, was submitted to three mutagenic agents: gamma radiation, ultraviolet light and nitrous acid. Survival curves were obtained and mutants were selected using different mutagenic doses which gave 1 to 5% survival. Morphological and auxotrophic mutants were isolated. Morphological mutants were grouped in a class with yellow conidia and other with pale vinaceous conidia as opposed to the green wild type conidia. Auxotrophic mutants had requirements for vitamin and aminoacid biosynthesis. More than 58% of the total auxotrophk mutants required proline/aipnine. Gamma radiation showed to be the most efficient mutagenic agent giving 0.2% of auxotrophk mutants followed by ultraviolet light (0.12%) and nitrous acid (0.06%).The conidial colour and auxotrophk mutants isolated until now from M. anisopliae were reviewed.
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spelling Survival and mutant production induced by mutagenic agents in Metarhizium anisopliaeMetarhizium anisopliaeauxotrophk mutantscolour mutantsmutagenic agentsgamma radiationultraviolet lightnitrous acidA wild strain of Metarhizium anisopliae, an entomopathogenic fungus, was submitted to three mutagenic agents: gamma radiation, ultraviolet light and nitrous acid. Survival curves were obtained and mutants were selected using different mutagenic doses which gave 1 to 5% survival. Morphological and auxotrophic mutants were isolated. Morphological mutants were grouped in a class with yellow conidia and other with pale vinaceous conidia as opposed to the green wild type conidia. Auxotrophic mutants had requirements for vitamin and aminoacid biosynthesis. More than 58% of the total auxotrophk mutants required proline/aipnine. Gamma radiation showed to be the most efficient mutagenic agent giving 0.2% of auxotrophk mutants followed by ultraviolet light (0.12%) and nitrous acid (0.06%).The conidial colour and auxotrophk mutants isolated until now from M. anisopliae were reviewed.Escola Superior de Agricultura "Luiz de Queiroz"1995-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161995000300023Scientia Agricola v.52 n.3 1995reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90161995000300023info:eu-repo/semantics/openAccessKava - Cordeiro,V.Luna - Alves - Lima,E.A.Azevedo,J.L.eng2005-06-23T00:00:00Zoai:scielo:S0103-90161995000300023Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2005-06-23T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae
title Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae
spellingShingle Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae
Kava - Cordeiro,V.
Metarhizium anisopliae
auxotrophk mutants
colour mutants
mutagenic agents
gamma radiation
ultraviolet light
nitrous acid
title_short Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae
title_full Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae
title_fullStr Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae
title_full_unstemmed Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae
title_sort Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae
author Kava - Cordeiro,V.
author_facet Kava - Cordeiro,V.
Luna - Alves - Lima,E.A.
Azevedo,J.L.
author_role author
author2 Luna - Alves - Lima,E.A.
Azevedo,J.L.
author2_role author
author
dc.contributor.author.fl_str_mv Kava - Cordeiro,V.
Luna - Alves - Lima,E.A.
Azevedo,J.L.
dc.subject.por.fl_str_mv Metarhizium anisopliae
auxotrophk mutants
colour mutants
mutagenic agents
gamma radiation
ultraviolet light
nitrous acid
topic Metarhizium anisopliae
auxotrophk mutants
colour mutants
mutagenic agents
gamma radiation
ultraviolet light
nitrous acid
description A wild strain of Metarhizium anisopliae, an entomopathogenic fungus, was submitted to three mutagenic agents: gamma radiation, ultraviolet light and nitrous acid. Survival curves were obtained and mutants were selected using different mutagenic doses which gave 1 to 5% survival. Morphological and auxotrophic mutants were isolated. Morphological mutants were grouped in a class with yellow conidia and other with pale vinaceous conidia as opposed to the green wild type conidia. Auxotrophic mutants had requirements for vitamin and aminoacid biosynthesis. More than 58% of the total auxotrophk mutants required proline/aipnine. Gamma radiation showed to be the most efficient mutagenic agent giving 0.2% of auxotrophk mutants followed by ultraviolet light (0.12%) and nitrous acid (0.06%).The conidial colour and auxotrophk mutants isolated until now from M. anisopliae were reviewed.
publishDate 1995
dc.date.none.fl_str_mv 1995-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161995000300023
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161995000300023
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-90161995000300023
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
dc.source.none.fl_str_mv Scientia Agricola v.52 n.3 1995
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
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