Cytogenetic mapping of the ALMT (aluminum-activated malate transporter) gene in wheat genotypes

Detalhes bibliográficos
Autor(a) principal: Oliveira,Érica Cristina de
Data de Publicação: 2020
Outros Autores: Pinto-Maglio,Cecília Alzira Ferreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000501102
Resumo: ABSTRACT: Presence of the ALMT1 (aluminum-activated malate transporter) gene confers resistance to aluminum toxicity in Triticum aestivum (common wheat). No resistant cultivars of Triticum turgidum ssp. Durum Desf. (durum wheat) have been registered in Brazil. The aim of this study was to map the ALMT1 through application of the FISH (fluorescence in situ hybridization) technique in five wheat genotypes, common and durum, from the Active Germplasm Bank (AGB) of the Instituto Agronômico (IAC): BH 1146, P19, P33, Anahuac, and IAC 1003. FISH-ALMT1 signals were registered in Anahuac (sensitive) chromosomes and in BH 1146, P19, and P33 (resistant) chromosomes. In the three resistant genotypes, a characteristic double FISH signal was found, located in different chromosomes of the complements: in BH 1146 in chromosome 5D, in P19 in 3B, and in P33 in 6B. This FISH - ALMT1 mapping allows for introgression of aluminum resistance in sensitive cultivars through breeding programs using introgression lines containing these carrier chromosomes.
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spelling Cytogenetic mapping of the ALMT (aluminum-activated malate transporter) gene in wheat genotypesTriticum sp.molecular cytogeneticsfluorescence in situ hybridizationaluminum tolerancetoxicityABSTRACT: Presence of the ALMT1 (aluminum-activated malate transporter) gene confers resistance to aluminum toxicity in Triticum aestivum (common wheat). No resistant cultivars of Triticum turgidum ssp. Durum Desf. (durum wheat) have been registered in Brazil. The aim of this study was to map the ALMT1 through application of the FISH (fluorescence in situ hybridization) technique in five wheat genotypes, common and durum, from the Active Germplasm Bank (AGB) of the Instituto Agronômico (IAC): BH 1146, P19, P33, Anahuac, and IAC 1003. FISH-ALMT1 signals were registered in Anahuac (sensitive) chromosomes and in BH 1146, P19, and P33 (resistant) chromosomes. In the three resistant genotypes, a characteristic double FISH signal was found, located in different chromosomes of the complements: in BH 1146 in chromosome 5D, in P19 in 3B, and in P33 in 6B. This FISH - ALMT1 mapping allows for introgression of aluminum resistance in sensitive cultivars through breeding programs using introgression lines containing these carrier chromosomes.Escola Superior de Agricultura "Luiz de Queiroz"2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000501102Scientia Agricola v.77 n.5 2020reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-992x-2019-0012info:eu-repo/semantics/openAccessOliveira,Érica Cristina dePinto-Maglio,Cecília Alzira Ferreiraeng2019-12-12T00:00:00Zoai:scielo:S0103-90162020000501102Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2019-12-12T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Cytogenetic mapping of the ALMT (aluminum-activated malate transporter) gene in wheat genotypes
title Cytogenetic mapping of the ALMT (aluminum-activated malate transporter) gene in wheat genotypes
spellingShingle Cytogenetic mapping of the ALMT (aluminum-activated malate transporter) gene in wheat genotypes
Oliveira,Érica Cristina de
Triticum sp.
molecular cytogenetics
fluorescence in situ hybridization
aluminum tolerance
toxicity
title_short Cytogenetic mapping of the ALMT (aluminum-activated malate transporter) gene in wheat genotypes
title_full Cytogenetic mapping of the ALMT (aluminum-activated malate transporter) gene in wheat genotypes
title_fullStr Cytogenetic mapping of the ALMT (aluminum-activated malate transporter) gene in wheat genotypes
title_full_unstemmed Cytogenetic mapping of the ALMT (aluminum-activated malate transporter) gene in wheat genotypes
title_sort Cytogenetic mapping of the ALMT (aluminum-activated malate transporter) gene in wheat genotypes
author Oliveira,Érica Cristina de
author_facet Oliveira,Érica Cristina de
Pinto-Maglio,Cecília Alzira Ferreira
author_role author
author2 Pinto-Maglio,Cecília Alzira Ferreira
author2_role author
dc.contributor.author.fl_str_mv Oliveira,Érica Cristina de
Pinto-Maglio,Cecília Alzira Ferreira
dc.subject.por.fl_str_mv Triticum sp.
molecular cytogenetics
fluorescence in situ hybridization
aluminum tolerance
toxicity
topic Triticum sp.
molecular cytogenetics
fluorescence in situ hybridization
aluminum tolerance
toxicity
description ABSTRACT: Presence of the ALMT1 (aluminum-activated malate transporter) gene confers resistance to aluminum toxicity in Triticum aestivum (common wheat). No resistant cultivars of Triticum turgidum ssp. Durum Desf. (durum wheat) have been registered in Brazil. The aim of this study was to map the ALMT1 through application of the FISH (fluorescence in situ hybridization) technique in five wheat genotypes, common and durum, from the Active Germplasm Bank (AGB) of the Instituto Agronômico (IAC): BH 1146, P19, P33, Anahuac, and IAC 1003. FISH-ALMT1 signals were registered in Anahuac (sensitive) chromosomes and in BH 1146, P19, and P33 (resistant) chromosomes. In the three resistant genotypes, a characteristic double FISH signal was found, located in different chromosomes of the complements: in BH 1146 in chromosome 5D, in P19 in 3B, and in P33 in 6B. This FISH - ALMT1 mapping allows for introgression of aluminum resistance in sensitive cultivars through breeding programs using introgression lines containing these carrier chromosomes.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000501102
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000501102
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-992x-2019-0012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
dc.source.none.fl_str_mv Scientia Agricola v.77 n.5 2020
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
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