Usefulness of aqueous and vitreous humor analysis in infectious uveitis

• Autor(a) principal: Santos, Helen Nazareth Veloso dos
• Data de Publicação: 2020
• Outros Autores: Ferracioli-Oda, Eduardo, Barbosa, Thaisa Silveira, Otani, Camila Sayuri Vicentini, Tanaka, Tatiana, Silva, Luciane de Carvalho Sarahyba da, Lopes, Guilherme de Oliveira, Doi, Andre, Hirata, Carlos Eduardo, Yamamoto, Joyce Hisae
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Clinics
• Texto Completo: https://www.revistas.usp.br/clinics/article/view/167130
• topic: Polymerase Chain Reaction; Infectious Disease; Uveitis; Aqueous Humor; Vitreous Humor

OBJECTIVE: To evaluate the role of intraocular fluid analysis as a diagnostic aid for uveitis. METHODS: Twenty-eight samples (27 patients including 3 HIV-infected patients) with active (n=24) or non-active (n=4) uveitis were submitted to aqueous (AH; n=12) or vitreous humor (VH) analysis (n=16). All samples were analyzed by quantitative PCR for herpes simplex virus (HSV), varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein-Barr virus (EBV) and Toxoplasma gondii. RESULTS: The positivity of the PCR in AH was 41.7% (5/12), with 50% (2/4) in immunocompetent and 67% (2/3) in HIV+ patients. The positivity of the PCR in VH was 31.2% (5/16), with 13% (1/8) in immunocompetent and 50% (4/8) in immunosuppressed HIV negative patients. The analysis was a determinant in the diagnostic definition in 58% of HA and 50% of VH. CONCLUSION: Even in posterior uveitis, initial AH analysis may be helpful. A careful formulation of possible clinical diagnosis seems to increase the chance of intraocular sample analysis being meaningful.