Adrenomedulline improves ischemic left colonic anastomotic healing in an experimental rodent model
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/19496 |
Resumo: | BACKGROUND: Leakage from colonic anastomosis is a major complication causing increased mortality and morbidity. Ischemia is a well-known cause of this event. This study was designed to investigate the effects of adrenomedullin on the healing of ischemic colon anastomosis in a rat model. METHODS: Standardized left colon resection 3 cm above the peritoneal reflection and colonic anastomosis were performed in 40 Wistar rats that were divided into four groups. To mimic ischemia, the mesocolon was ligated 2 cm from either side of the anastomosis in all of the groups. The control groups (1 and 2) received no further treatment. The experimental groups (3 and 4) received adrenomedullin treatment. Adrenomedullin therapy was started in the perioperative period in group 3 and 4 rats (the therapeutic groups). Group 1 and group 3 rats were sacrificed on postoperative day 3. Group 2 and group 4 rats were sacrificed on postoperative day 7. After careful relaparotomy, bursting pressure, hydroxyproline, malondialdehyde, interleukin 6, nitric oxide, vascular endothelial growth factor, and tumor necrosis factor alpha levels were measured. Histopathological characteristics of the anastomosis were analyzed. RESULTS: The group 3 animals had a significantly higher bursting pressure than group 1 (p |
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Adrenomedulline improves ischemic left colonic anastomotic healing in an experimental rodent model Colonic anastomosisIschemiaAdrenomedullinOxidative damageNeovascularization BACKGROUND: Leakage from colonic anastomosis is a major complication causing increased mortality and morbidity. Ischemia is a well-known cause of this event. This study was designed to investigate the effects of adrenomedullin on the healing of ischemic colon anastomosis in a rat model. METHODS: Standardized left colon resection 3 cm above the peritoneal reflection and colonic anastomosis were performed in 40 Wistar rats that were divided into four groups. To mimic ischemia, the mesocolon was ligated 2 cm from either side of the anastomosis in all of the groups. The control groups (1 and 2) received no further treatment. The experimental groups (3 and 4) received adrenomedullin treatment. Adrenomedullin therapy was started in the perioperative period in group 3 and 4 rats (the therapeutic groups). Group 1 and group 3 rats were sacrificed on postoperative day 3. Group 2 and group 4 rats were sacrificed on postoperative day 7. After careful relaparotomy, bursting pressure, hydroxyproline, malondialdehyde, interleukin 6, nitric oxide, vascular endothelial growth factor, and tumor necrosis factor alpha levels were measured. Histopathological characteristics of the anastomosis were analyzed. RESULTS: The group 3 animals had a significantly higher bursting pressure than group 1 (pHospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1949610.1590/S1807-59322011001000021Clinics; Vol. 66 No. 10 (2011); 1805-1810 Clinics; v. 66 n. 10 (2011); 1805-1810 Clinics; Vol. 66 Núm. 10 (2011); 1805-1810 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/19496/21559Karatepe, OguzhanKurtulus, IdrisYalcin, OrhanBattal, MuharremKamali, GulcinAydin, Timucininfo:eu-repo/semantics/openAccess2012-05-23T16:44:03Zoai:revistas.usp.br:article/19496Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-23T16:44:03Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Adrenomedulline improves ischemic left colonic anastomotic healing in an experimental rodent model |
title |
Adrenomedulline improves ischemic left colonic anastomotic healing in an experimental rodent model |
spellingShingle |
Adrenomedulline improves ischemic left colonic anastomotic healing in an experimental rodent model Karatepe, Oguzhan Colonic anastomosis Ischemia Adrenomedullin Oxidative damage Neovascularization |
title_short |
Adrenomedulline improves ischemic left colonic anastomotic healing in an experimental rodent model |
title_full |
Adrenomedulline improves ischemic left colonic anastomotic healing in an experimental rodent model |
title_fullStr |
Adrenomedulline improves ischemic left colonic anastomotic healing in an experimental rodent model |
title_full_unstemmed |
Adrenomedulline improves ischemic left colonic anastomotic healing in an experimental rodent model |
title_sort |
Adrenomedulline improves ischemic left colonic anastomotic healing in an experimental rodent model |
author |
Karatepe, Oguzhan |
author_facet |
Karatepe, Oguzhan Kurtulus, Idris Yalcin, Orhan Battal, Muharrem Kamali, Gulcin Aydin, Timucin |
author_role |
author |
author2 |
Kurtulus, Idris Yalcin, Orhan Battal, Muharrem Kamali, Gulcin Aydin, Timucin |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Karatepe, Oguzhan Kurtulus, Idris Yalcin, Orhan Battal, Muharrem Kamali, Gulcin Aydin, Timucin |
dc.subject.por.fl_str_mv |
Colonic anastomosis Ischemia Adrenomedullin Oxidative damage Neovascularization |
topic |
Colonic anastomosis Ischemia Adrenomedullin Oxidative damage Neovascularization |
description |
BACKGROUND: Leakage from colonic anastomosis is a major complication causing increased mortality and morbidity. Ischemia is a well-known cause of this event. This study was designed to investigate the effects of adrenomedullin on the healing of ischemic colon anastomosis in a rat model. METHODS: Standardized left colon resection 3 cm above the peritoneal reflection and colonic anastomosis were performed in 40 Wistar rats that were divided into four groups. To mimic ischemia, the mesocolon was ligated 2 cm from either side of the anastomosis in all of the groups. The control groups (1 and 2) received no further treatment. The experimental groups (3 and 4) received adrenomedullin treatment. Adrenomedullin therapy was started in the perioperative period in group 3 and 4 rats (the therapeutic groups). Group 1 and group 3 rats were sacrificed on postoperative day 3. Group 2 and group 4 rats were sacrificed on postoperative day 7. After careful relaparotomy, bursting pressure, hydroxyproline, malondialdehyde, interleukin 6, nitric oxide, vascular endothelial growth factor, and tumor necrosis factor alpha levels were measured. Histopathological characteristics of the anastomosis were analyzed. RESULTS: The group 3 animals had a significantly higher bursting pressure than group 1 (p |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/19496 10.1590/S1807-59322011001000021 |
url |
https://www.revistas.usp.br/clinics/article/view/19496 |
identifier_str_mv |
10.1590/S1807-59322011001000021 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/19496/21559 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 66 No. 10 (2011); 1805-1810 Clinics; v. 66 n. 10 (2011); 1805-1810 Clinics; Vol. 66 Núm. 10 (2011); 1805-1810 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222757359714304 |