Standardization of a spinal cord lesion model and neurologic evaluation using mice

Detalhes bibliográficos
Autor(a) principal: Borges, Paulo Alvim
Data de Publicação: 2018
Outros Autores: Cristante, Alexandre Fogaça, Barros-Filho, Tarcísio Eloy Pessoa de, Natalino, Renato Jose Mendonça, Santos, Gustavo Bispo dos, Marcon, Raphael Marcus
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/144532
Resumo: OBJECTIVE: To standardize a spinal cord lesion mouse model. METHODS: Thirty BALB/c mice were divided into five groups: four experimental groups and one control group (sham). The experimental groups were subjected to spinal cord lesion by a weight drop from different heights after laminectomy whereas the sham group only underwent laminectomy. Mice were observed for six weeks, and functional behavior scales were applied. The mice were then euthanized, and histological investigations were performed to confirm and score spinal cord lesion. The findings were evaluated to prove whether the method of administering spinal cord lesion was effective and different among the groups. Additionally, we correlated the results of the functional scales with the results from the histology evaluations to identify which scale is more reliable. RESULTS: One mouse presented autophagia, and six mice died during the experiment. Because four of the mice that died were in Group 5, Group 5 was excluded from the study. All the functional scales assessed proved to be significantly different from each other, and mice presented functional evolution during the experiment. Spinal cord lesion was confirmed by histology, and the results showed a high correlation between the Basso, Beattie, Bresnahan Locomotor Rating Scale and the Basso Mouse Scale. The mouse function scale showed a moderate to high correlation with the histological findings, and the horizontal ladder test had a high correlation with neurologic degeneration but no correlation with the other histological parameters evaluated. CONCLUSION: This spinal cord lesion mouse model proved to be effective and reliable with exception of lesions caused by a 10-g drop from 50 mm, which resulted in unacceptable mortality. The Basso, Beattie, Bresnahan Locomotor Rating Scale and Basso Mouse Scale are the most reliable functional assessments, and but the horizontal ladder test is not recommended.
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spelling Standardization of a spinal cord lesion model and neurologic evaluation using miceReference StandardsMiceSpinal CordSpinal Cord CompressionOBJECTIVE: To standardize a spinal cord lesion mouse model. METHODS: Thirty BALB/c mice were divided into five groups: four experimental groups and one control group (sham). The experimental groups were subjected to spinal cord lesion by a weight drop from different heights after laminectomy whereas the sham group only underwent laminectomy. Mice were observed for six weeks, and functional behavior scales were applied. The mice were then euthanized, and histological investigations were performed to confirm and score spinal cord lesion. The findings were evaluated to prove whether the method of administering spinal cord lesion was effective and different among the groups. Additionally, we correlated the results of the functional scales with the results from the histology evaluations to identify which scale is more reliable. RESULTS: One mouse presented autophagia, and six mice died during the experiment. Because four of the mice that died were in Group 5, Group 5 was excluded from the study. All the functional scales assessed proved to be significantly different from each other, and mice presented functional evolution during the experiment. Spinal cord lesion was confirmed by histology, and the results showed a high correlation between the Basso, Beattie, Bresnahan Locomotor Rating Scale and the Basso Mouse Scale. The mouse function scale showed a moderate to high correlation with the histological findings, and the horizontal ladder test had a high correlation with neurologic degeneration but no correlation with the other histological parameters evaluated. CONCLUSION: This spinal cord lesion mouse model proved to be effective and reliable with exception of lesions caused by a 10-g drop from 50 mm, which resulted in unacceptable mortality. The Basso, Beattie, Bresnahan Locomotor Rating Scale and Basso Mouse Scale are the most reliable functional assessments, and but the horizontal ladder test is not recommended.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/14453210.6061/clinics/2018/e293Clinics; Vol. 73 (2018); e293Clinics; v. 73 (2018); e293Clinics; Vol. 73 (2018); e2931980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/144532/138859Copyright (c) 2018 Clinicsinfo:eu-repo/semantics/openAccessBorges, Paulo AlvimCristante, Alexandre FogaçaBarros-Filho, Tarcísio Eloy Pessoa deNatalino, Renato Jose MendonçaSantos, Gustavo Bispo dosMarcon, Raphael Marcus2019-05-14T11:48:50Zoai:revistas.usp.br:article/144532Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2019-05-14T11:48:50Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Standardization of a spinal cord lesion model and neurologic evaluation using mice
title Standardization of a spinal cord lesion model and neurologic evaluation using mice
spellingShingle Standardization of a spinal cord lesion model and neurologic evaluation using mice
Borges, Paulo Alvim
Reference Standards
Mice
Spinal Cord
Spinal Cord Compression
title_short Standardization of a spinal cord lesion model and neurologic evaluation using mice
title_full Standardization of a spinal cord lesion model and neurologic evaluation using mice
title_fullStr Standardization of a spinal cord lesion model and neurologic evaluation using mice
title_full_unstemmed Standardization of a spinal cord lesion model and neurologic evaluation using mice
title_sort Standardization of a spinal cord lesion model and neurologic evaluation using mice
author Borges, Paulo Alvim
author_facet Borges, Paulo Alvim
Cristante, Alexandre Fogaça
Barros-Filho, Tarcísio Eloy Pessoa de
Natalino, Renato Jose Mendonça
Santos, Gustavo Bispo dos
Marcon, Raphael Marcus
author_role author
author2 Cristante, Alexandre Fogaça
Barros-Filho, Tarcísio Eloy Pessoa de
Natalino, Renato Jose Mendonça
Santos, Gustavo Bispo dos
Marcon, Raphael Marcus
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Borges, Paulo Alvim
Cristante, Alexandre Fogaça
Barros-Filho, Tarcísio Eloy Pessoa de
Natalino, Renato Jose Mendonça
Santos, Gustavo Bispo dos
Marcon, Raphael Marcus
dc.subject.por.fl_str_mv Reference Standards
Mice
Spinal Cord
Spinal Cord Compression
topic Reference Standards
Mice
Spinal Cord
Spinal Cord Compression
description OBJECTIVE: To standardize a spinal cord lesion mouse model. METHODS: Thirty BALB/c mice were divided into five groups: four experimental groups and one control group (sham). The experimental groups were subjected to spinal cord lesion by a weight drop from different heights after laminectomy whereas the sham group only underwent laminectomy. Mice were observed for six weeks, and functional behavior scales were applied. The mice were then euthanized, and histological investigations were performed to confirm and score spinal cord lesion. The findings were evaluated to prove whether the method of administering spinal cord lesion was effective and different among the groups. Additionally, we correlated the results of the functional scales with the results from the histology evaluations to identify which scale is more reliable. RESULTS: One mouse presented autophagia, and six mice died during the experiment. Because four of the mice that died were in Group 5, Group 5 was excluded from the study. All the functional scales assessed proved to be significantly different from each other, and mice presented functional evolution during the experiment. Spinal cord lesion was confirmed by histology, and the results showed a high correlation between the Basso, Beattie, Bresnahan Locomotor Rating Scale and the Basso Mouse Scale. The mouse function scale showed a moderate to high correlation with the histological findings, and the horizontal ladder test had a high correlation with neurologic degeneration but no correlation with the other histological parameters evaluated. CONCLUSION: This spinal cord lesion mouse model proved to be effective and reliable with exception of lesions caused by a 10-g drop from 50 mm, which resulted in unacceptable mortality. The Basso, Beattie, Bresnahan Locomotor Rating Scale and Basso Mouse Scale are the most reliable functional assessments, and but the horizontal ladder test is not recommended.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/144532
10.6061/clinics/2018/e293
url https://www.revistas.usp.br/clinics/article/view/144532
identifier_str_mv 10.6061/clinics/2018/e293
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/144532/138859
dc.rights.driver.fl_str_mv Copyright (c) 2018 Clinics
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Clinics
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 73 (2018); e293
Clinics; v. 73 (2018); e293
Clinics; Vol. 73 (2018); e293
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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