The influence of sleep deprivation and obesity on DNA damage in female Zucker rats
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/72138 |
Resumo: | OBJECTIVE: The aim of this study was to evaluate overall genetic damage induced by total sleep deprivation in obese, female Zucker rats of differing ages. METHOD: Lean and obese Zucker rats at 3, 6, and 15 months old were randomly distributed into two groups for each age group: home-cage control and sleep-deprived (N = 5/group). The sleep-deprived groups were deprived sleep by gentle handling for 6 hours, whereas the home-cage control group was allowed to remain undisturbed in their home-cage. At the end of the sleep deprivation period, or after an equivalent amount of time for the home-cage control groups, the rats were brought to an adjacent room and decapitated. The blood, brain, and liver tissue were collected and stored individually to evaluate DNA damage. RESULTS: Significant genetic damage was observed only in 15-month-old rats. Genetic damage was present in the liver cells from sleep-deprived obese rats compared with lean rats in the same condition. Sleep deprivation was associated with genetic damage in brain cells regardless of obesity status. DNA damage was observed in the peripheral blood cells regardless of sleep condition or obesity status. CONCLUSION: Taken together, these results suggest that obesity was associated with genetic damage in liver cells, whereas sleep deprivation was associated with DNA damage in brain cells. These results also indicate that there is no synergistic effect of these noxious conditions on the overall level of genetic damage. In addition, the level of DNA damage was significantly higher in 15-month-old rats compared to younger rats. |
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Clinics |
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The influence of sleep deprivation and obesity on DNA damage in female Zucker ratsSleep DeprivationObesityZucker RatsDNA DamageComet AssayGentle HandlingFemaleOBJECTIVE: The aim of this study was to evaluate overall genetic damage induced by total sleep deprivation in obese, female Zucker rats of differing ages. METHOD: Lean and obese Zucker rats at 3, 6, and 15 months old were randomly distributed into two groups for each age group: home-cage control and sleep-deprived (N = 5/group). The sleep-deprived groups were deprived sleep by gentle handling for 6 hours, whereas the home-cage control group was allowed to remain undisturbed in their home-cage. At the end of the sleep deprivation period, or after an equivalent amount of time for the home-cage control groups, the rats were brought to an adjacent room and decapitated. The blood, brain, and liver tissue were collected and stored individually to evaluate DNA damage. RESULTS: Significant genetic damage was observed only in 15-month-old rats. Genetic damage was present in the liver cells from sleep-deprived obese rats compared with lean rats in the same condition. Sleep deprivation was associated with genetic damage in brain cells regardless of obesity status. DNA damage was observed in the peripheral blood cells regardless of sleep condition or obesity status. CONCLUSION: Taken together, these results suggest that obesity was associated with genetic damage in liver cells, whereas sleep deprivation was associated with DNA damage in brain cells. These results also indicate that there is no synergistic effect of these noxious conditions on the overall level of genetic damage. In addition, the level of DNA damage was significantly higher in 15-month-old rats compared to younger rats.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/7213810.1590/clin.v68i3.72138Clinics; Vol. 68 No. 3 (2013); 385-389Clinics; v. 68 n. 3 (2013); 385-389Clinics; Vol. 68 Núm. 3 (2013); 385-3891980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/72138/75373Tenorio, Neuli M.Ribeiro, Daniel A.Alvarenga, Tathiana A.Fracalossi, Ana Carolina C.Carlin, VivianeHirotsu, CamilaTufik, SergioAndersen, Monica L.info:eu-repo/semantics/openAccess2014-01-28T17:05:37Zoai:revistas.usp.br:article/72138Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2014-01-28T17:05:37Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
The influence of sleep deprivation and obesity on DNA damage in female Zucker rats |
title |
The influence of sleep deprivation and obesity on DNA damage in female Zucker rats |
spellingShingle |
The influence of sleep deprivation and obesity on DNA damage in female Zucker rats Tenorio, Neuli M. Sleep Deprivation Obesity Zucker Rats DNA Damage Comet Assay Gentle Handling Female |
title_short |
The influence of sleep deprivation and obesity on DNA damage in female Zucker rats |
title_full |
The influence of sleep deprivation and obesity on DNA damage in female Zucker rats |
title_fullStr |
The influence of sleep deprivation and obesity on DNA damage in female Zucker rats |
title_full_unstemmed |
The influence of sleep deprivation and obesity on DNA damage in female Zucker rats |
title_sort |
The influence of sleep deprivation and obesity on DNA damage in female Zucker rats |
author |
Tenorio, Neuli M. |
author_facet |
Tenorio, Neuli M. Ribeiro, Daniel A. Alvarenga, Tathiana A. Fracalossi, Ana Carolina C. Carlin, Viviane Hirotsu, Camila Tufik, Sergio Andersen, Monica L. |
author_role |
author |
author2 |
Ribeiro, Daniel A. Alvarenga, Tathiana A. Fracalossi, Ana Carolina C. Carlin, Viviane Hirotsu, Camila Tufik, Sergio Andersen, Monica L. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Tenorio, Neuli M. Ribeiro, Daniel A. Alvarenga, Tathiana A. Fracalossi, Ana Carolina C. Carlin, Viviane Hirotsu, Camila Tufik, Sergio Andersen, Monica L. |
dc.subject.por.fl_str_mv |
Sleep Deprivation Obesity Zucker Rats DNA Damage Comet Assay Gentle Handling Female |
topic |
Sleep Deprivation Obesity Zucker Rats DNA Damage Comet Assay Gentle Handling Female |
description |
OBJECTIVE: The aim of this study was to evaluate overall genetic damage induced by total sleep deprivation in obese, female Zucker rats of differing ages. METHOD: Lean and obese Zucker rats at 3, 6, and 15 months old were randomly distributed into two groups for each age group: home-cage control and sleep-deprived (N = 5/group). The sleep-deprived groups were deprived sleep by gentle handling for 6 hours, whereas the home-cage control group was allowed to remain undisturbed in their home-cage. At the end of the sleep deprivation period, or after an equivalent amount of time for the home-cage control groups, the rats were brought to an adjacent room and decapitated. The blood, brain, and liver tissue were collected and stored individually to evaluate DNA damage. RESULTS: Significant genetic damage was observed only in 15-month-old rats. Genetic damage was present in the liver cells from sleep-deprived obese rats compared with lean rats in the same condition. Sleep deprivation was associated with genetic damage in brain cells regardless of obesity status. DNA damage was observed in the peripheral blood cells regardless of sleep condition or obesity status. CONCLUSION: Taken together, these results suggest that obesity was associated with genetic damage in liver cells, whereas sleep deprivation was associated with DNA damage in brain cells. These results also indicate that there is no synergistic effect of these noxious conditions on the overall level of genetic damage. In addition, the level of DNA damage was significantly higher in 15-month-old rats compared to younger rats. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/72138 10.1590/clin.v68i3.72138 |
url |
https://www.revistas.usp.br/clinics/article/view/72138 |
identifier_str_mv |
10.1590/clin.v68i3.72138 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/72138/75373 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 68 No. 3 (2013); 385-389 Clinics; v. 68 n. 3 (2013); 385-389 Clinics; Vol. 68 Núm. 3 (2013); 385-389 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222759811284992 |