Hypothalamic transcriptional expression of the kisspeptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes

Detalhes bibliográficos
Autor(a) principal: Marcondes, Rodrigo Rodrigues
Data de Publicação: 2017
Outros Autores: Carvalho, Kátia Cândido, Giannocco, Gisele, Duarte, Daniele Coelho, Garcia, Natália, Soares-Junior, José Maria, da Silva, Ismael Dale Cotrim Guerreiro, Maliqueo, Manuel, Baracat, Edmund Chada, Maciel, Gustavo Arantes Rosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/138277
Resumo: OBJECTIVES: Polycystic ovary syndrome is a heterogeneous endocrine disorder that affects reproductive-age women. The mechanisms underlying the endocrine heterogeneity and neuroendocrinology of polycystic ovary syndrome are still unclear. In this study, we investigated the expression of the kisspeptin system and gonadotropin-releasing hormone pulse regulators in the hypothalamus as well as factors related to luteinizing hormone secretion in the pituitary of polycystic ovary syndrome rat models induced by testosterone or estradiol. METHODS: A single injection of testosterone propionate (1.25 mg) (n=10) or estradiol benzoate (0.5 mg) (n=10) was administered to female rats at 2 days of age to induce experimental polycystic ovary syndrome. Controls were injected with a vehicle (n=10). Animals were euthanized at 90-94 days of age, and the hypothalamus and pituitary gland were used for gene expression analysis. RESULTS: Rats exposed to testosterone exhibited increased transcriptional expression of the androgen receptor and estrogen receptor-β and reduced expression of kisspeptin in the hypothalamus. However, rats exposed to estradiol did not show any significant changes in hormone levels relative to controls but exhibited hypothalamic downregulation of kisspeptin, tachykinin 3 and estrogen receptor-α genes and upregulation of the gene that encodes the kisspeptin receptor. CONCLUSIONS: Testosterone- and estradiol-exposed rats with different endocrine phenotypes showed differential transcriptional expression of members of the kisspeptin system and sex steroid receptors in the hypothalamus. These differences might account for the different endocrine phenotypes found in testosterone- and estradiol-induced polycystic ovary syndrome rats.
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spelling Hypothalamic transcriptional expression of the kisspeptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypesPolycystic Ovary SyndromeHypothalamusAnimal ModelsKisspeptinTestosteroneOBJECTIVES: Polycystic ovary syndrome is a heterogeneous endocrine disorder that affects reproductive-age women. The mechanisms underlying the endocrine heterogeneity and neuroendocrinology of polycystic ovary syndrome are still unclear. In this study, we investigated the expression of the kisspeptin system and gonadotropin-releasing hormone pulse regulators in the hypothalamus as well as factors related to luteinizing hormone secretion in the pituitary of polycystic ovary syndrome rat models induced by testosterone or estradiol. METHODS: A single injection of testosterone propionate (1.25 mg) (n=10) or estradiol benzoate (0.5 mg) (n=10) was administered to female rats at 2 days of age to induce experimental polycystic ovary syndrome. Controls were injected with a vehicle (n=10). Animals were euthanized at 90-94 days of age, and the hypothalamus and pituitary gland were used for gene expression analysis. RESULTS: Rats exposed to testosterone exhibited increased transcriptional expression of the androgen receptor and estrogen receptor-β and reduced expression of kisspeptin in the hypothalamus. However, rats exposed to estradiol did not show any significant changes in hormone levels relative to controls but exhibited hypothalamic downregulation of kisspeptin, tachykinin 3 and estrogen receptor-α genes and upregulation of the gene that encodes the kisspeptin receptor. CONCLUSIONS: Testosterone- and estradiol-exposed rats with different endocrine phenotypes showed differential transcriptional expression of members of the kisspeptin system and sex steroid receptors in the hypothalamus. These differences might account for the different endocrine phenotypes found in testosterone- and estradiol-induced polycystic ovary syndrome rats.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2017-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/13827710.6061/clinics/2017(08)09Clinics; Vol. 72 No. 8 (2017); 510-514Clinics; v. 72 n. 8 (2017); 510-514Clinics; Vol. 72 Núm. 8 (2017); 510-5141980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/138277/133718Copyright (c) 2017 Clinicsinfo:eu-repo/semantics/openAccessMarcondes, Rodrigo RodriguesCarvalho, Kátia CândidoGiannocco, GiseleDuarte, Daniele CoelhoGarcia, NatáliaSoares-Junior, José Mariada Silva, Ismael Dale Cotrim GuerreiroMaliqueo, ManuelBaracat, Edmund ChadaMaciel, Gustavo Arantes Rosa2017-09-22T16:20:24Zoai:revistas.usp.br:article/138277Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2017-09-22T16:20:24Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Hypothalamic transcriptional expression of the kisspeptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes
title Hypothalamic transcriptional expression of the kisspeptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes
spellingShingle Hypothalamic transcriptional expression of the kisspeptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes
Marcondes, Rodrigo Rodrigues
Polycystic Ovary Syndrome
Hypothalamus
Animal Models
Kisspeptin
Testosterone
title_short Hypothalamic transcriptional expression of the kisspeptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes
title_full Hypothalamic transcriptional expression of the kisspeptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes
title_fullStr Hypothalamic transcriptional expression of the kisspeptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes
title_full_unstemmed Hypothalamic transcriptional expression of the kisspeptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes
title_sort Hypothalamic transcriptional expression of the kisspeptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes
author Marcondes, Rodrigo Rodrigues
author_facet Marcondes, Rodrigo Rodrigues
Carvalho, Kátia Cândido
Giannocco, Gisele
Duarte, Daniele Coelho
Garcia, Natália
Soares-Junior, José Maria
da Silva, Ismael Dale Cotrim Guerreiro
Maliqueo, Manuel
Baracat, Edmund Chada
Maciel, Gustavo Arantes Rosa
author_role author
author2 Carvalho, Kátia Cândido
Giannocco, Gisele
Duarte, Daniele Coelho
Garcia, Natália
Soares-Junior, José Maria
da Silva, Ismael Dale Cotrim Guerreiro
Maliqueo, Manuel
Baracat, Edmund Chada
Maciel, Gustavo Arantes Rosa
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Marcondes, Rodrigo Rodrigues
Carvalho, Kátia Cândido
Giannocco, Gisele
Duarte, Daniele Coelho
Garcia, Natália
Soares-Junior, José Maria
da Silva, Ismael Dale Cotrim Guerreiro
Maliqueo, Manuel
Baracat, Edmund Chada
Maciel, Gustavo Arantes Rosa
dc.subject.por.fl_str_mv Polycystic Ovary Syndrome
Hypothalamus
Animal Models
Kisspeptin
Testosterone
topic Polycystic Ovary Syndrome
Hypothalamus
Animal Models
Kisspeptin
Testosterone
description OBJECTIVES: Polycystic ovary syndrome is a heterogeneous endocrine disorder that affects reproductive-age women. The mechanisms underlying the endocrine heterogeneity and neuroendocrinology of polycystic ovary syndrome are still unclear. In this study, we investigated the expression of the kisspeptin system and gonadotropin-releasing hormone pulse regulators in the hypothalamus as well as factors related to luteinizing hormone secretion in the pituitary of polycystic ovary syndrome rat models induced by testosterone or estradiol. METHODS: A single injection of testosterone propionate (1.25 mg) (n=10) or estradiol benzoate (0.5 mg) (n=10) was administered to female rats at 2 days of age to induce experimental polycystic ovary syndrome. Controls were injected with a vehicle (n=10). Animals were euthanized at 90-94 days of age, and the hypothalamus and pituitary gland were used for gene expression analysis. RESULTS: Rats exposed to testosterone exhibited increased transcriptional expression of the androgen receptor and estrogen receptor-β and reduced expression of kisspeptin in the hypothalamus. However, rats exposed to estradiol did not show any significant changes in hormone levels relative to controls but exhibited hypothalamic downregulation of kisspeptin, tachykinin 3 and estrogen receptor-α genes and upregulation of the gene that encodes the kisspeptin receptor. CONCLUSIONS: Testosterone- and estradiol-exposed rats with different endocrine phenotypes showed differential transcriptional expression of members of the kisspeptin system and sex steroid receptors in the hypothalamus. These differences might account for the different endocrine phenotypes found in testosterone- and estradiol-induced polycystic ovary syndrome rats.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/138277
10.6061/clinics/2017(08)09
url https://www.revistas.usp.br/clinics/article/view/138277
identifier_str_mv 10.6061/clinics/2017(08)09
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/138277/133718
dc.rights.driver.fl_str_mv Copyright (c) 2017 Clinics
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Clinics
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 72 No. 8 (2017); 510-514
Clinics; v. 72 n. 8 (2017); 510-514
Clinics; Vol. 72 Núm. 8 (2017); 510-514
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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