Importance of detection of Strongyloides stercoralis DNA in fecal samples from patients with type 2 diabetes mellitus
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/213513 |
Resumo: | Objective: The association between diabetes and Strongyloides infection remains controversial. This study aimed to detect Strongyloides stercoralis DNA in the feces of patients with Diabetes Mellitus type 2 (DM2). Methods: Fecal samples were analyzed via the Lutz, Rugai, and agar plate culture methods. PCR amplification was performed using two targets (PCR-genus and PCR-species) located on the S. stercoralis 18S ribosomal. Results: The positivity for S. stercoralis using parasitological methods was 1.1%. PCR-genus (14.13%) demonstrated a higher positivity than PCR-species (9.78%). Conclusion: The results confirm the greater positivity of the molecular diagnosis in relation to parasitological methods, reinforcing its use as an additional tool for the diagnosis of S. stercoralis infection in patients with DM2 living in endemic areas for this helminthiasis. |
id |
USP-19_757ee0482d5db9cd41a31205fee1c347 |
---|---|
oai_identifier_str |
oai:revistas.usp.br:article/213513 |
network_acronym_str |
USP-19 |
network_name_str |
Clinics |
repository_id_str |
|
spelling |
Importance of detection of Strongyloides stercoralis DNA in fecal samples from patients with type 2 diabetes mellitusStrongyloidiasiDiabetes mellitusParasitological diagnosticMolecular diagnosticObjective: The association between diabetes and Strongyloides infection remains controversial. This study aimed to detect Strongyloides stercoralis DNA in the feces of patients with Diabetes Mellitus type 2 (DM2). Methods: Fecal samples were analyzed via the Lutz, Rugai, and agar plate culture methods. PCR amplification was performed using two targets (PCR-genus and PCR-species) located on the S. stercoralis 18S ribosomal. Results: The positivity for S. stercoralis using parasitological methods was 1.1%. PCR-genus (14.13%) demonstrated a higher positivity than PCR-species (9.78%). Conclusion: The results confirm the greater positivity of the molecular diagnosis in relation to parasitological methods, reinforcing its use as an additional tool for the diagnosis of S. stercoralis infection in patients with DM2 living in endemic areas for this helminthiasis.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2022-07-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/21351310.1016/j.clinsp.2022.100060Clinics; Vol. 77 (2022); 100060Clinics; v. 77 (2022); 100060Clinics; Vol. 77 (2022); 1000601980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/213513/195611Copyright (c) 2023 Clinicsinfo:eu-repo/semantics/openAccessMazzaro, Márcia CarolinaSantos, Émelin Alves dosMelo, Gessica Baptista deMarques, Priscila DuarteSouza, Laura VilelaElias-Oliveira, JeffersonSilva, Bruna Campos daGryschek, Ronaldo César BorgesPaula, Fabiana Martins deRodrigues, Rosângela Maria2023-07-06T13:04:57Zoai:revistas.usp.br:article/213513Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2023-07-06T13:04:57Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Importance of detection of Strongyloides stercoralis DNA in fecal samples from patients with type 2 diabetes mellitus |
title |
Importance of detection of Strongyloides stercoralis DNA in fecal samples from patients with type 2 diabetes mellitus |
spellingShingle |
Importance of detection of Strongyloides stercoralis DNA in fecal samples from patients with type 2 diabetes mellitus Mazzaro, Márcia Carolina Strongyloidiasi Diabetes mellitus Parasitological diagnostic Molecular diagnostic |
title_short |
Importance of detection of Strongyloides stercoralis DNA in fecal samples from patients with type 2 diabetes mellitus |
title_full |
Importance of detection of Strongyloides stercoralis DNA in fecal samples from patients with type 2 diabetes mellitus |
title_fullStr |
Importance of detection of Strongyloides stercoralis DNA in fecal samples from patients with type 2 diabetes mellitus |
title_full_unstemmed |
Importance of detection of Strongyloides stercoralis DNA in fecal samples from patients with type 2 diabetes mellitus |
title_sort |
Importance of detection of Strongyloides stercoralis DNA in fecal samples from patients with type 2 diabetes mellitus |
author |
Mazzaro, Márcia Carolina |
author_facet |
Mazzaro, Márcia Carolina Santos, Émelin Alves dos Melo, Gessica Baptista de Marques, Priscila Duarte Souza, Laura Vilela Elias-Oliveira, Jefferson Silva, Bruna Campos da Gryschek, Ronaldo César Borges Paula, Fabiana Martins de Rodrigues, Rosângela Maria |
author_role |
author |
author2 |
Santos, Émelin Alves dos Melo, Gessica Baptista de Marques, Priscila Duarte Souza, Laura Vilela Elias-Oliveira, Jefferson Silva, Bruna Campos da Gryschek, Ronaldo César Borges Paula, Fabiana Martins de Rodrigues, Rosângela Maria |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Mazzaro, Márcia Carolina Santos, Émelin Alves dos Melo, Gessica Baptista de Marques, Priscila Duarte Souza, Laura Vilela Elias-Oliveira, Jefferson Silva, Bruna Campos da Gryschek, Ronaldo César Borges Paula, Fabiana Martins de Rodrigues, Rosângela Maria |
dc.subject.por.fl_str_mv |
Strongyloidiasi Diabetes mellitus Parasitological diagnostic Molecular diagnostic |
topic |
Strongyloidiasi Diabetes mellitus Parasitological diagnostic Molecular diagnostic |
description |
Objective: The association between diabetes and Strongyloides infection remains controversial. This study aimed to detect Strongyloides stercoralis DNA in the feces of patients with Diabetes Mellitus type 2 (DM2). Methods: Fecal samples were analyzed via the Lutz, Rugai, and agar plate culture methods. PCR amplification was performed using two targets (PCR-genus and PCR-species) located on the S. stercoralis 18S ribosomal. Results: The positivity for S. stercoralis using parasitological methods was 1.1%. PCR-genus (14.13%) demonstrated a higher positivity than PCR-species (9.78%). Conclusion: The results confirm the greater positivity of the molecular diagnosis in relation to parasitological methods, reinforcing its use as an additional tool for the diagnosis of S. stercoralis infection in patients with DM2 living in endemic areas for this helminthiasis. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-11 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/213513 10.1016/j.clinsp.2022.100060 |
url |
https://www.revistas.usp.br/clinics/article/view/213513 |
identifier_str_mv |
10.1016/j.clinsp.2022.100060 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/213513/195611 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Clinics info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Clinics |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 77 (2022); 100060 Clinics; v. 77 (2022); 100060 Clinics; Vol. 77 (2022); 100060 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222766650097664 |