Mechanism underlying Müller cell pyroptosis and its role in the development of proliferative vitreoretinopathy
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/214039 |
Resumo: | Objectives: To explore the mechanism underlying Müller Cell Pyroptosis (MCP) and its role in the development of Proliferative Vitreoretinopathy (PVR). Method: The expression of pyroptosis-related factors, namely, cysteinyl aspartate-specific proteinase (caspase-1), interleukin (IL)-1β, IL-18, and Gasdermin D (GSDMD), was detected by quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) and western blotting at the mRNA and protein levels, respectively, in retinal tissues. Müller and spontaneously Arising Retinal Pigment Epithelia (ARPE)-19 primary cells with GSDMD overexpression or knockdown were cultivated. Western blotting was used to detect the levels of the following pyroptosis-related factors in retinal tissues: caspase-1, IL-1β, IL-18, and GSDMD. Through Cell Adhesion (CA) experiments, the changes in ARPE-19 CA in each group were observed. The migration and invasion of ARPE-19 cells were measured using the Transwell assay. The proliferation of ARPE-19 cells was measured with a Cell Counting Kit 8 (CCK-8) assay. Finally, the expression of the cytokines IL-1β and IL-18 in the ARPE-19 cell culture medium was detected using the Enzyme-Linked Immunosorbent Assay (ELISA). Results: Compared with the surrounding normal tissues, the expression of caspase-1, IL-1β, IL-18, and GSDMD at the protein and mRNA levels in the retinal proliferative membrane samples of the patients decreased significantly (p < 0.05). MCP significantly enhanced ARPE-19 CA, migration and invasion, proliferation, and cytokine expression (p < 0.05). Conclusions: MCP can promote the development of PVR lesions. |
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Clinics |
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Mechanism underlying Müller cell pyroptosis and its role in the development of proliferative vitreoretinopathyMüller cellsPyroptosisProliferative vitreoretinopathyObjectives: To explore the mechanism underlying Müller Cell Pyroptosis (MCP) and its role in the development of Proliferative Vitreoretinopathy (PVR). Method: The expression of pyroptosis-related factors, namely, cysteinyl aspartate-specific proteinase (caspase-1), interleukin (IL)-1β, IL-18, and Gasdermin D (GSDMD), was detected by quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) and western blotting at the mRNA and protein levels, respectively, in retinal tissues. Müller and spontaneously Arising Retinal Pigment Epithelia (ARPE)-19 primary cells with GSDMD overexpression or knockdown were cultivated. Western blotting was used to detect the levels of the following pyroptosis-related factors in retinal tissues: caspase-1, IL-1β, IL-18, and GSDMD. Through Cell Adhesion (CA) experiments, the changes in ARPE-19 CA in each group were observed. The migration and invasion of ARPE-19 cells were measured using the Transwell assay. The proliferation of ARPE-19 cells was measured with a Cell Counting Kit 8 (CCK-8) assay. Finally, the expression of the cytokines IL-1β and IL-18 in the ARPE-19 cell culture medium was detected using the Enzyme-Linked Immunosorbent Assay (ELISA). Results: Compared with the surrounding normal tissues, the expression of caspase-1, IL-1β, IL-18, and GSDMD at the protein and mRNA levels in the retinal proliferative membrane samples of the patients decreased significantly (p < 0.05). MCP significantly enhanced ARPE-19 CA, migration and invasion, proliferation, and cytokine expression (p < 0.05). Conclusions: MCP can promote the development of PVR lesions.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2023-07-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/21403910.1016/j.clinsp.2023.100241Clinics; Vol. 78 (2023); 100241Clinics; v. 78 (2023); 100241Clinics; Vol. 78 (2023); 1002411980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/214039/196274Copyright (c) 2023 Clinicsinfo:eu-repo/semantics/openAccessBai, YueXie, MaosongZhu, Yihua2023-07-06T13:05:40Zoai:revistas.usp.br:article/214039Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2023-07-06T13:05:40Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Mechanism underlying Müller cell pyroptosis and its role in the development of proliferative vitreoretinopathy |
title |
Mechanism underlying Müller cell pyroptosis and its role in the development of proliferative vitreoretinopathy |
spellingShingle |
Mechanism underlying Müller cell pyroptosis and its role in the development of proliferative vitreoretinopathy Bai, Yue Müller cells Pyroptosis Proliferative vitreoretinopathy |
title_short |
Mechanism underlying Müller cell pyroptosis and its role in the development of proliferative vitreoretinopathy |
title_full |
Mechanism underlying Müller cell pyroptosis and its role in the development of proliferative vitreoretinopathy |
title_fullStr |
Mechanism underlying Müller cell pyroptosis and its role in the development of proliferative vitreoretinopathy |
title_full_unstemmed |
Mechanism underlying Müller cell pyroptosis and its role in the development of proliferative vitreoretinopathy |
title_sort |
Mechanism underlying Müller cell pyroptosis and its role in the development of proliferative vitreoretinopathy |
author |
Bai, Yue |
author_facet |
Bai, Yue Xie, Maosong Zhu, Yihua |
author_role |
author |
author2 |
Xie, Maosong Zhu, Yihua |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Bai, Yue Xie, Maosong Zhu, Yihua |
dc.subject.por.fl_str_mv |
Müller cells Pyroptosis Proliferative vitreoretinopathy |
topic |
Müller cells Pyroptosis Proliferative vitreoretinopathy |
description |
Objectives: To explore the mechanism underlying Müller Cell Pyroptosis (MCP) and its role in the development of Proliferative Vitreoretinopathy (PVR). Method: The expression of pyroptosis-related factors, namely, cysteinyl aspartate-specific proteinase (caspase-1), interleukin (IL)-1β, IL-18, and Gasdermin D (GSDMD), was detected by quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) and western blotting at the mRNA and protein levels, respectively, in retinal tissues. Müller and spontaneously Arising Retinal Pigment Epithelia (ARPE)-19 primary cells with GSDMD overexpression or knockdown were cultivated. Western blotting was used to detect the levels of the following pyroptosis-related factors in retinal tissues: caspase-1, IL-1β, IL-18, and GSDMD. Through Cell Adhesion (CA) experiments, the changes in ARPE-19 CA in each group were observed. The migration and invasion of ARPE-19 cells were measured using the Transwell assay. The proliferation of ARPE-19 cells was measured with a Cell Counting Kit 8 (CCK-8) assay. Finally, the expression of the cytokines IL-1β and IL-18 in the ARPE-19 cell culture medium was detected using the Enzyme-Linked Immunosorbent Assay (ELISA). Results: Compared with the surrounding normal tissues, the expression of caspase-1, IL-1β, IL-18, and GSDMD at the protein and mRNA levels in the retinal proliferative membrane samples of the patients decreased significantly (p < 0.05). MCP significantly enhanced ARPE-19 CA, migration and invasion, proliferation, and cytokine expression (p < 0.05). Conclusions: MCP can promote the development of PVR lesions. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-05 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/214039 10.1016/j.clinsp.2023.100241 |
url |
https://www.revistas.usp.br/clinics/article/view/214039 |
identifier_str_mv |
10.1016/j.clinsp.2023.100241 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/214039/196274 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Clinics info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Clinics |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 78 (2023); 100241 Clinics; v. 78 (2023); 100241 Clinics; Vol. 78 (2023); 100241 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222767420801024 |