Transmission electron microscopy of the preclinical phase of experimental phytophotodermatitis
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/17808 |
Resumo: | OBJECTIVE: To examine the epidermis in induced phytophotodermatitis using transmission electron microscopy in order to detect histologic changes even before lesions are visible by light microscopy. INTRODUCTION: In the first six hours after the experimental induction of phytophotodermatitis, no changes are detectable by light microscopy. Only after 24 hours can keratinocyte necrosis and epidermal vacuolization be detected histologically, and blisters form by 48 hours. METHODS: The dorsum of four adult rats (Rattus norvegicus) was manually epilated. After painting the right half of the rat with the peel juice of Tahiti lemon, they were exposed to sunlight for eight minutes under general anesthesia. The left side was used as the control and exposed to sunlight only. Biopsies were performed immediately after photoinduction and one and two hours later, and the tissue was analyzed by transmission electron microscopy. RESULTS: No histological changes were seen on the control side. Immediately after induction, vacuolization in keratinocytes was observed. After one hour, desmosomal changes were also observed in addition to vacuolization. Keratin filaments were not attached to the desmosomal plaque. Free desmosomes and membrane ruptures were also seen. At two hours after induction, similar changes were found, and granular degeneration of keratin was also observed. DISCUSSION: The interaction of sunlight and psoralens generates a photoproduct that damages keratinocyte proteins, leading to keratinocyte necrosis and blister formation. CONCLUSIONS: Transmission electron microscopy can detect vacuolization, lesions of the membrane, and desmosomes in the first two hours after experimental induction of phytophotodermatitis. |
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Clinics |
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Transmission electron microscopy of the preclinical phase of experimental phytophotodermatitis PhytophotodermatitisAnimal ModelTransmission Electron MicroscopyDesmosomesKeratinocytes OBJECTIVE: To examine the epidermis in induced phytophotodermatitis using transmission electron microscopy in order to detect histologic changes even before lesions are visible by light microscopy. INTRODUCTION: In the first six hours after the experimental induction of phytophotodermatitis, no changes are detectable by light microscopy. Only after 24 hours can keratinocyte necrosis and epidermal vacuolization be detected histologically, and blisters form by 48 hours. METHODS: The dorsum of four adult rats (Rattus norvegicus) was manually epilated. After painting the right half of the rat with the peel juice of Tahiti lemon, they were exposed to sunlight for eight minutes under general anesthesia. The left side was used as the control and exposed to sunlight only. Biopsies were performed immediately after photoinduction and one and two hours later, and the tissue was analyzed by transmission electron microscopy. RESULTS: No histological changes were seen on the control side. Immediately after induction, vacuolization in keratinocytes was observed. After one hour, desmosomal changes were also observed in addition to vacuolization. Keratin filaments were not attached to the desmosomal plaque. Free desmosomes and membrane ruptures were also seen. At two hours after induction, similar changes were found, and granular degeneration of keratin was also observed. DISCUSSION: The interaction of sunlight and psoralens generates a photoproduct that damages keratinocyte proteins, leading to keratinocyte necrosis and blister formation. CONCLUSIONS: Transmission electron microscopy can detect vacuolization, lesions of the membrane, and desmosomes in the first two hours after experimental induction of phytophotodermatitis. Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1780810.1590/S1807-59322008000300014Clinics; Vol. 63 No. 3 (2008); 371-374 Clinics; v. 63 n. 3 (2008); 371-374 Clinics; Vol. 63 Núm. 3 (2008); 371-374 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/17808/19873Almeida Jr, Hiram Larangeira deSotto, Miriam NakagamiCastro, Luis Antonio Suita deRocha, Nara Moreirainfo:eu-repo/semantics/openAccess2012-05-22T18:35:12Zoai:revistas.usp.br:article/17808Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-22T18:35:12Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Transmission electron microscopy of the preclinical phase of experimental phytophotodermatitis |
title |
Transmission electron microscopy of the preclinical phase of experimental phytophotodermatitis |
spellingShingle |
Transmission electron microscopy of the preclinical phase of experimental phytophotodermatitis Almeida Jr, Hiram Larangeira de Phytophotodermatitis Animal Model Transmission Electron Microscopy Desmosomes Keratinocytes |
title_short |
Transmission electron microscopy of the preclinical phase of experimental phytophotodermatitis |
title_full |
Transmission electron microscopy of the preclinical phase of experimental phytophotodermatitis |
title_fullStr |
Transmission electron microscopy of the preclinical phase of experimental phytophotodermatitis |
title_full_unstemmed |
Transmission electron microscopy of the preclinical phase of experimental phytophotodermatitis |
title_sort |
Transmission electron microscopy of the preclinical phase of experimental phytophotodermatitis |
author |
Almeida Jr, Hiram Larangeira de |
author_facet |
Almeida Jr, Hiram Larangeira de Sotto, Miriam Nakagami Castro, Luis Antonio Suita de Rocha, Nara Moreira |
author_role |
author |
author2 |
Sotto, Miriam Nakagami Castro, Luis Antonio Suita de Rocha, Nara Moreira |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Almeida Jr, Hiram Larangeira de Sotto, Miriam Nakagami Castro, Luis Antonio Suita de Rocha, Nara Moreira |
dc.subject.por.fl_str_mv |
Phytophotodermatitis Animal Model Transmission Electron Microscopy Desmosomes Keratinocytes |
topic |
Phytophotodermatitis Animal Model Transmission Electron Microscopy Desmosomes Keratinocytes |
description |
OBJECTIVE: To examine the epidermis in induced phytophotodermatitis using transmission electron microscopy in order to detect histologic changes even before lesions are visible by light microscopy. INTRODUCTION: In the first six hours after the experimental induction of phytophotodermatitis, no changes are detectable by light microscopy. Only after 24 hours can keratinocyte necrosis and epidermal vacuolization be detected histologically, and blisters form by 48 hours. METHODS: The dorsum of four adult rats (Rattus norvegicus) was manually epilated. After painting the right half of the rat with the peel juice of Tahiti lemon, they were exposed to sunlight for eight minutes under general anesthesia. The left side was used as the control and exposed to sunlight only. Biopsies were performed immediately after photoinduction and one and two hours later, and the tissue was analyzed by transmission electron microscopy. RESULTS: No histological changes were seen on the control side. Immediately after induction, vacuolization in keratinocytes was observed. After one hour, desmosomal changes were also observed in addition to vacuolization. Keratin filaments were not attached to the desmosomal plaque. Free desmosomes and membrane ruptures were also seen. At two hours after induction, similar changes were found, and granular degeneration of keratin was also observed. DISCUSSION: The interaction of sunlight and psoralens generates a photoproduct that damages keratinocyte proteins, leading to keratinocyte necrosis and blister formation. CONCLUSIONS: Transmission electron microscopy can detect vacuolization, lesions of the membrane, and desmosomes in the first two hours after experimental induction of phytophotodermatitis. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/17808 10.1590/S1807-59322008000300014 |
url |
https://www.revistas.usp.br/clinics/article/view/17808 |
identifier_str_mv |
10.1590/S1807-59322008000300014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/17808/19873 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 63 No. 3 (2008); 371-374 Clinics; v. 63 n. 3 (2008); 371-374 Clinics; Vol. 63 Núm. 3 (2008); 371-374 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222753905704960 |